PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1113 [new locus tag: SAUSA300_RS06025 ]
• pan locus tag^?: SAUPAN003503000
• symbol: pknB
• pan gene symbol^?: pknB
• synonym:
• product: protein kinase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1113 [new locus tag: SAUSA300_RS06025 ]
• symbol: pknB
• product: protein kinase
• replicon: chromosome
• strand: +
• coordinates: 1218149..1220143
• length: 1995
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914306 NCBI
• RefSeq: YP_493810 NCBI
• BioCyc: see SAUSA300_RS06025
• MicrobesOnline: 1292628 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  1561
  1621
  1681
  1741
  1801
  1861
  1921
  1981
  
  ATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGC
  ATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCG
  ATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTTGAACGAGAAGTACAT
  AACTCATCACAGCTATCACATCAAAATATAGTAAGTATGATCGATGTTGATGAAGAAGAT
  GACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAA
  AGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGC
  ATTAAACATGCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTA
  ATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAG
  ACGTCTTTAACTCAGACTAATCATGTGTTAGGTACTGTGCAGTACTTTTCGCCAGAACAA
  GCAAAAGGTGAGGCAACGGATGAATGTACAGATATTTATTCTATAGGTATTGTGTTATAT
  GAAATGCTTGTTGGTGAACCACCCTTTAATGGAGAAACTGCAGTTAGCATTGCGATTAAA
  CATATTCAGGATTCTGTGCCAAATGTGACAACAGATGTACGTAAGGATATTCCGCAATCT
  TTAAGTAATGTCATTTTACGCGCTACAGAAAAAGACAAAGCGAATCGTTACAAAACAATT
  CAAGAAATGAAAGATGATTTGAGTAGTGTTTTACATGAAAATCGAGCGAATGAAGATGTC
  TATGAACTCGATAAAATGAAAACGATAGCGGTACCTTTGAAAAAAGAAGATCTAGCAAAG
  CATATTAGTGAACATAAGTCGAATCAACCTAAACGTGAAACGACGCAAGTACCTATTGTA
  AATGGGCCTGCTCATCATCAGCAATTCCAAAAGCCAGAAGGTACGGTGTACGAACCAAAA
  CCTAAAAAGAAATCAACACGAAAGATTGTGCTCTTATCACTAATCTTTTCGTTGTTAATG
  ATTGCACTTGTTTCTTTTGTGGCAATGGCAATGTTTGGTAATAAATACGAAGAGACACCT
  GATGTAATCGGGAAATCTGTAAAAGAAGCAGAGCAAATATTCAATAAAAACAACCTGAAA
  TTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATCCTGAAAATGAAATTATTAAGACA
  ACTCCTAATACTGGTGAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAG
  GGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAG
  AAATTAAAATCGTTAGGTCTTAAAGATGTTACGATTGAAAAAGTATATAATAATCAAGCG
  CCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGAT
  TCTAATATTAAACTATATGAATCTTTAGGCATTAAGCAAGTTTATGTAGAAGACTTTGAA
  CATAAATCCTTTAGCAAAGCTAAAAAAGCCTTAGAAGAAAAAGGGTTTAAAGTTGAAAGT
  AAGGAAGAGTATAGTGACGATATTGATGAGGGTGATGTGATTTCTCAATCTCCTAAAGGA
  AAATCAGTAGATGAGGGGTCAACGATTTCATTTGTTGTTTCTAAAGGTAAAAAAAGTGAC
  TCATCAGATGTCAAAACGACAACTGAATCGGTAGATGTACCATACACTGGTAAAAATGAT
  AAGTCACAAAAAGTTAAAGTTTATATTAAAGATAAAGATAATGACGGTTCAACTGAAAAA
  GGTAGTTTCGATATTACTAGTGATCAACGTATAGACATTCCTTTAAGAATTGAAAAAGGA
  AAAACAGCAAGTTATATTGTTAAAGTTGACGGTAAAACTGTAGCTGAAAAAGAAGTCAGC
  TATGATGATGTATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1500
  1560
  1620
  1680
  1740
  1800
  1860
  1920
  1980
  1995
  

⊟Protein

⊟General

• locus tag: SAUSA300_1113 [new locus tag: SAUSA300_RS06025 ]
• symbol: PknB
• description: protein kinase
• length: 664
• theoretical pI: 5.9417
• theoretical MW: 74362.8
• GRAVY: -0.546837

⊟Function

• reaction:
  EC 2.7.1.-?  ExPASy
• TIGRFAM:
  Cellular processes Cellular processes Toxin production and resistance TOMM system kinase/cyclase fusion protein (TIGR03903; HMM-score: 150.6)
  and 3 more
  Unknown function General Kae1-associated kinase Bud32 (TIGR03724; EC 2.7.-.-; HMM-score: 41.9)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-polyprenylphenol 6-hydroxylase (TIGR01982; EC 1.14.13.-; HMM-score: 16.9)
  early transcribed membrane protein (ETRAMP) family (TIGR01495; HMM-score: 14.5)
• TheSEED  :
  • Serine/threonine protein kinase PrkC, regulator of stationary phase
  A Gram-positive cluster that relates ribosomal protein L28P to a set of uncharacterized proteins  Serine/threonine protein kinase PrkC, regulator of stationary phase
  and 1 more
  Conserved gene cluster associated with Met-tRNA formyltransferase  Serine/threonine protein kinase PrkC, regulator of stationary phase
• PFAM:
  PKinase (CL0016) Pkinase; Protein kinase domain (PF00069; HMM-score: 196.3)
  and 12 more
  no clan defined PASTA; PASTA domain (PF03793; HMM-score: 145.1)
  PKinase (CL0016) Pkinase_Tyr; Protein tyrosine kinase (PF07714; HMM-score: 134.6)
  APH; Phosphotransferase enzyme family (PF01636; HMM-score: 27)
  Seadorna_VP7; Seadornavirus VP7 (PF07387; HMM-score: 15.7)
  RIO1; RIO1 family (PF01163; HMM-score: 15.2)
  no clan defined Sigma_reg_N; Sigma factor regulator N-terminal (PF13800; HMM-score: 15.2)
  ChlamPMP_M; Chlamydia polymorphic membrane protein middle domain (PF07548; HMM-score: 14.9)
  PKinase (CL0016) Choline_kinase; Choline/ethanolamine kinase (PF01633; HMM-score: 14.7)
  Kinase-like; Kinase-like (PF14531; HMM-score: 13.8)
  no clan defined ETRAMP; Malarial early transcribed membrane protein (ETRAMP) (PF09716; HMM-score: 13.4)
  PKinase (CL0016) Kdo; Lipopolysaccharide kinase (Kdo/WaaP) family (PF06293; HMM-score: 12.8)
  no clan defined PNPase; Polyribonucleotide nucleotidyltransferase, RNA binding domain (PF03726; HMM-score: 12.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.17
  • Cytoplasmic Membrane Score: 9.51
  • Cellwall Score: 0.16
  • Extracellular Score: 0.15
  • Internal Helix: 1
• LocateP: Intracellular /TMH start AFTER 60
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: Possibly Sec-
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002877
  • TAT(Tat/SPI): 0.000159
  • LIPO(Sec/SPII): 0.00064
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 87161100 NCBI
• RefSeq: YP_493810 NCBI
• UniProt: A0A0H2XGG5 UniProt

⊟Protein sequence

• MIGKIINERYKIVDKLGGGGMSTVYLAEDTILNIKVAIKAIFIPPREKEETLKRFEREVHNSSQLSHQNIVSMIDVDEEDDCYYLVMEYIEGPTLSEYIESHGPLSVDTAINFTNQILDGIKHAHDMRIVHRDIKPQNILIDSNKTLKIFDFGIAKALSETSLTQTNHVLGTVQYFSPEQAKGEATDECTDIYSIGIVLYEMLVGEPPFNGETAVSIAIKHIQDSVPNVTTDVRKDIPQSLSNVILRATEKDKANRYKTIQEMKDDLSSVLHENRANEDVYELDKMKTIAVPLKKEDLAKHISEHKSNQPKRETTQVPIVNGPAHHQQFQKPEGTVYEPKPKKKSTRKIVLLSLIFSLLMIALVSFVAMAMFGNKYEETPDVIGKSVKEAEQIFNKNNLKLGKISRSYSDKYPENEIIKTTPNTGERVERGDSVDVVISKGPEKVKMPNVIGLPKEEALQKLKSLGLKDVTIEKVYNNQAPKGYIANQSVTANTEIAIHDSNIKLYESLGIKQVYVEDFEHKSFSKAKKALEEKGFKVESKEEYSDDIDEGDVISQSPKGKSVDEGSTISFVVSKGKKSDSSDVKTTTESVDVPYTGKNDKSQKVKVYIKDKDNDGSTEKGSFDITSDQRIDIPLRIEKGKTASYIVKVDGKTVAEKEVSYDDV

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: def > fmt > sun > SAUSA300_1111 > SAUSA300_1112 > pknB

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications