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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0974 [new locus tag: SAUSA300_RS05235 ]
  • pan locus tag?: SAUPAN003285000
  • symbol: purN
  • pan gene symbol?: purN
  • synonym:
  • product: phosphoribosylglycinamide formyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0974 [new locus tag: SAUSA300_RS05235 ]
  • symbol: purN
  • product: phosphoribosylglycinamide formyltransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 1067353..1067919
  • length: 567
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGGTTAAAATTGCGATTTTTGCATCAGGTTCAGGAAGTAACTTTGAAAATATAGTTGAG
    CATGTTGAATCAGGAAAACTTGAAAATATTGAAGTTACGGCGCTATATACGGATCATCAA
    AATGCGTTTTGTATAGATAGAGCAAAAAAGCACGATATTCCTGTTTATATTAATGAACCA
    AAACAATTTGATTCAAAAGCAGCGTATGAACAACATTTAGTAACATTATTAAATGAAGAT
    AAGGTAGAGTGGATTATTCTAGCTGGCTACATGCGTCTAATTGGTCCAGACTTATTAGCT
    TCATTTGAAGGTAAAATATTGAATATACATCCATCTCTATTGCCGAAATATAAGGGGATT
    GACGCAATAGGCCAAGCATATCATAGTGGCGATACTATTACTGGTTCGACAGTACATTAT
    GTTGATAGTGGTATGGATACGGGAGAAATTATTGAACAGAGAAAATGTGATATTAGACCG
    GACGATTCAAAAGAACAATTAGAAGAGAAAGTAAAAAAATTGGAATATGAGTTATATCCA
    AGTGTTATTGCTAAAATTGTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    567

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_0974 [new locus tag: SAUSA300_RS05235 ]
  • symbol: PurN
  • description: phosphoribosylglycinamide formyltransferase
  • length: 188
  • theoretical pI: 5.11422
  • theoretical MW: 21150.9
  • GRAVY: -0.301064

⊟Function[edit | edit source]

  • reaction:
    EC 2.1.2.2?  ExPASy
    Phosphoribosylglycinamide formyltransferase 10-formyltetrahydrofolate + N1-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide
  • TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylglycinamide formyltransferase (TIGR00639; EC 2.1.2.2; HMM-score: 214.4)
    and 3 more
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis formyltetrahydrofolate deformylase (TIGR00655; EC 3.5.1.10; HMM-score: 102.6)
    Genetic information processing Protein synthesis tRNA aminoacylation methionyl-tRNA formyltransferase (TIGR00460; EC 2.1.2.9; HMM-score: 90.7)
    phosphoglycerol geranylgeranyltransferase, putative (TIGR04147; EC 2.5.1.41; HMM-score: 13)
  • TheSEED  :
    • Phosphoribosylglycinamide formyltransferase (EC 2.1.2.2)
    Nucleosides and Nucleotides Purines De Novo Purine Biosynthesis  Phosphoribosylglycinamide formyltransferase (EC 2.1.2.2)
    and 1 more
    Potassium metabolism Potassium metabolism - no subcategory Glutathione-regulated potassium-efflux system and associated functions  Phosphoribosylglycinamide formyltransferase (EC 2.1.2.2)
  • PFAM:
    no clan defined Formyl_trans_N; Formyl transferase (PF00551; HMM-score: 190.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.011916
    • TAT(Tat/SPI): 0.000427
    • LIPO(Sec/SPII): 0.001615
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MVKIAIFASGSGSNFENIVEHVESGKLENIEVTALYTDHQNAFCIDRAKKHDIPVYINEPKQFDSKAAYEQHLVTLLNEDKVEWIILAGYMRLIGPDLLASFEGKILNIHPSLLPKYKGIDAIGQAYHSGDTITGSTVHYVDSGMDTGEIIEQRKCDIRPDDSKEQLEEKVKKLEYELYPSVIAKIVK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • data available for N315

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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