PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0838 [new locus tag: SAUSA300_RS04530 ]
• pan locus tag^?: SAUPAN003034000
• symbol: dltD
• pan gene symbol^?: dltD
• synonym:
• product: D-alanine-activating enzyme/D-alanine-D-alanyl, dltD protein

⊟Additional information (user-provided)

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0838 [new locus tag: SAUSA300_RS04530 ]
• symbol: dltD
• product: D-alanine-activating enzyme/D-alanine-D-alanyl, dltD protein
• replicon: chromosome
• strand: +
• coordinates: 917331..918506
• length: 1176
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914191 NCBI
• RefSeq: YP_493538 NCBI
• BioCyc: see SAUSA300_RS04530
• MicrobesOnline: 1292353 MicrobesOnline

⊟Phenotype

⊟Additional information (user-provided)

⊟DNA sequence

• 1
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  ATGAAATTAAAACCTTTTTTACCCATTTTAATTAGTGGAGCGGTATTCATTGTCTTTCTA
  TTATTACCTGCTAGTTGGTTTACAGGATTAGTAAATGAAAAGACTGTAGAAGATAATAGA
  ACTTCATTGACAGATCAAGTACTAAAAGGCACACTCATTCAAGATAAGTTATACGAATCA
  AACAAGTATTATCCTATATACGGCTCTAGTGAATTAGGTAAAGATGACCCATTTAATCCT
  GCAATTGCATTAAATAAGCATAACGCCAACAAAAAAGCATTCTTATTAGGTGCTGGTGGT
  TCTACAGACTTAATTAACGCAGTTGAACTTGCATCACAGTATGATAAATTAAAAGGTAAG
  AAATTAACATTTATTATTTCACCACAATGGTTTACAAACCATGGTTTAACGAATCAAAAC
  TTTGATGCTCGTATGTCTCAAACTCAAATTAATCAAATGTTCCAGCAGAAAAACATGTCT
  ACTGAATTAAAACGTCGTTATGCACAACGTTTATTACAGTTTCCACATGTACACAATAAA
  GAATACTTGAAATCTTATGCTAAAAACCCTAAAGAAACTAAAGATAGTTATATTTCTGGT
  TTTAAAGAGAATCAATTGATTAAAATAGAAGCGATTAAATCATTGTTTGCAATGGATAAA
  TCTCCATTAGAACATGTTAAACCTGCTACAAAACCAGACGCTTCTTGGGATGAGATGAAA
  CAAAAAGCAGTTGAAATTGGTAAAGCTGATACTACATCGAATAAATTTGGTATTAGAGAT
  CAATACTGGAAATTAATTCAAGAAAGTAAGCGTAAAGTTAGACGTGACTACGAATTCAAT
  GTTAATTCTCCAGAATTCCAAGATTTAGAATTACTTGTAAAAACAATGCGTGCTGCTGGT
  GCAGATGTTCAATATGTAAGTATTCCATCAAACGGTGTATGGTATGACCACATTGGTATC
  GATAAAGAACGTCGTCAAGCAGTTTATAAAAAAATCCATTCTACTGTTGTAGATAATGGT
  GGTAAAATTTACGATATGACTGATAAAGAATATGAAAAATATGTTATCAGTGATGCCGTA
  CACATCGGTTGGAAAGGTTGGGTTTATATGGATGAGCAAATTGCGAAACATATGAAAGGT
  GAACCACAACCTGAAGTAGATAAACCTAAAAATTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_0838 [new locus tag: SAUSA300_RS04530 ]
• symbol: DltD
• description: D-alanine-activating enzyme/D-alanine-D-alanyl, dltD protein
• length: 391
• theoretical pI: 9.73378
• theoretical MW: 44960.1
• GRAVY: -0.634527

⊟Function

• TIGRFAM:
  D-alanyl-lipoteichoic acid biosynthesis protein DltD (TIGR04092; HMM-score: 377)
• TheSEED  :
  • Poly(glycerophosphate chain) D-alanine transfer protein DltD
  Cell Wall and Capsule Gram-Positive cell wall components D-Alanyl Lipoteichoic Acid Biosynthesis  Poly(glycerophosphate chain) D-alanine transfer protein DltD
  and 1 more
  Cell Wall and Capsule Gram-Positive cell wall components Teichoic and lipoteichoic acids biosynthesis  Poly(glycerophosphate chain) D-alanine transfer protein DltD
• PFAM:
  SGNH_hydrolase (CL0264) DltD; DltD protein (PF04914; HMM-score: 346.2)
  and 1 more
  Omega_toxin (CL0083) Chi-conotoxin; chi-Conotoxin or t superfamily (PF16981; HMM-score: 12.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helix: 1
• DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.0003
  • Cytoplasmic Membrane Score: 0.8559
  • Cell wall & surface Score: 0.0028
  • Extracellular Score: 0.141
• LocateP: N-terminally anchored (No CS)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 0
  • N-terminally Anchored Score: 2
  • Predicted Cleavage Site: No CleavageSite
• SignalP: Signal peptide SP(Sec/SPI) length 33 aa
  • SP(Sec/SPI): 0.459943
  • TAT(Tat/SPI): 0.007007
  • LIPO(Sec/SPII): 0.115123
  • Cleavage Site: CS pos: 33-34. VNE-KT. Pr: 0.0477
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 87160697 NCBI
• RefSeq: YP_493538 NCBI
• UniProt: A0A0H2XH34 UniProt

⊟Additional information (user-provided)

⊟Protein sequence

• MKLKPFLPILISGAVFIVFLLLPASWFTGLVNEKTVEDNRTSLTDQVLKGTLIQDKLYESNKYYPIYGSSELGKDDPFNPAIALNKHNANKKAFLLGAGGSTDLINAVELASQYDKLKGKKLTFIISPQWFTNHGLTNQNFDARMSQTQINQMFQQKNMSTELKRRYAQRLLQFPHVHNKEYLKSYAKNPKETKDSYISGFKENQLIKIEAIKSLFAMDKSPLEHVKPATKPDASWDEMKQKAVEIGKADTTSNKFGIRDQYWKLIQESKRKVRRDYEFNVNSPEFQDLELLVKTMRAAGADVQYVSIPSNGVWYDHIGIDKERRQAVYKKIHSTVVDNGGKIYDMTDKEYEKYVISDAVHIGWKGWVYMDEQIAKHMKGEPQPEVDKPKN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: dltA > dltB > dltC > dltD

⊟Regulation

• regulator:

⊟Additional information (user-provided)

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Additional information (user-provided)

⊟Other information (user-provided)

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications