PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0753 [new locus tag: SAUSA300_RS04065 ]
• pan locus tag^?: SAUPAN002697000
• symbol: SAUSA300_0753
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0753 [new locus tag: SAUSA300_RS04065 ]
• symbol: SAUSA300_0753
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 839406..840308
• length: 903
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3915005 NCBI
• RefSeq: YP_493454 NCBI
• BioCyc: see SAUSA300_RS04065
• MicrobesOnline: 1292268 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
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  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  
  ATGAAACAATACTTAATTACTGGTGGGACTGGTATGGTTGGATCTCAATTAGTTAATGAA
  ATTAAAAAATCAGATTCACATATCACGATATTAACGCGACACGACCAAATTTCAAATGAT
  AAGAAAATTTCATATGTCAACTGGGCTAAATCTGGGTGGGAACACAAAGTTCCTCAAAAT
  ATCGATGTGGTCATCAACTTAGCAGGTGCTACATTGAATAAACGATGGACACCGGAATAT
  AAACAAACGCTAATGCTAAGTAGAATTCAATCTACGCAAGCTTTATATGAATTGTTTAAA
  TCACGTAATAAAGCACCTAAAGCTTTATTTAATGCTAGTGCCACTGGTTACTATCCTCCT
  GATTTATTTATGAGTTACACAGAAGTTTATAAAACTTTACCTTTTGATTTCTTATCAGAT
  ATTGTGTATCAATGGGAACGTTTCGCACAACAATTTGAACAATTAGGTACACGAGTTGTC
  ATCGGTCGTTTCGGTATAATTTTATCGAATGAAGGCGGTGCGTTACAAACAATGAAACTA
  CCATACGAATATTACATTGGTGGTAAATTAGGTTCTGGTCAACAATGGTATTCATGGATT
  CATATCAATGATTTAATTCAAGCTATTTTATTTTTAATAAATAACGAGTCAGCTAGTGGT
  CCGTTTAATTTAACTGCACCTATACCTGAACGTCAAAATTTATTTGGCTACACTTTAGCA
  AGAGCTATGCATAAGCCTCATGAAACTTGGGCACCAAGTCTTGCAATGCGTCTCATACTT
  GGTCAAATGTCAACAGTAGTATTGGATACTCAAAAAGTATTACCTAATAAAATTCAAGCA
  TTGGGATTCCAATTTAAATATAGTAATTTAAAAATGGCACTTGAAGATTTAATTAAAGAA
  TAA
  60
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  903
  

⊟Protein

⊟General

• locus tag: SAUSA300_0753 [new locus tag: SAUSA300_RS04065 ]
• symbol: SAUSA300_0753
• description: hypothetical protein
• length: 300
• theoretical pI: 9.72292
• theoretical MW: 34227.2
• GRAVY: -0.227

⊟Function

• TIGRFAM:
  Hypothetical proteins Conserved TIGR01777 family protein (TIGR01777; HMM-score: 361.9)
  and 5 more
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides ADP-glyceromanno-heptose 6-epimerase (TIGR02197; EC 5.1.3.20; HMM-score: 29.7)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides dTDP-glucose 4,6-dehydratase (TIGR01181; EC 4.2.1.46; HMM-score: 20.2)
  hopanoid-associated sugar epimerase (TIGR03466; HMM-score: 15.5)
  Metabolism Energy metabolism Sugars UDP-glucose 4-epimerase GalE (TIGR01179; EC 5.1.3.2; HMM-score: 14.3)
  thioester reductase domain (TIGR01746; HMM-score: 12.7)
• TheSEED  :
  • Cell division inhibitor Slr1223 (YfcH in EC), contains epimerase/dehydratase and DUF1731 domains
• PFAM:
  NADP_Rossmann (CL0063) Epimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 67.6)
  no clan defined DUF1731; Domain of unknown function (DUF1731) (PF08338; HMM-score: 62)
  and 8 more
  NADP_Rossmann (CL0063) NAD_binding_4; Male sterility protein (PF07993; HMM-score: 24.9)
  GDP_Man_Dehyd; GDP-mannose 4,6 dehydratase (PF16363; HMM-score: 20.7)
  KR; KR domain (PF08659; HMM-score: 18)
  RmlD_sub_bind; RmlD substrate binding domain (PF04321; HMM-score: 15.5)
  Semialdhyde_dh; Semialdehyde dehydrogenase, NAD binding domain (PF01118; HMM-score: 14.4)
  Polysacc_synt_2; Polysaccharide biosynthesis protein (PF02719; HMM-score: 14.3)
  NmrA; NmrA-like family (PF05368; HMM-score: 12.9)
  NAD_binding_10; NAD(P)H-binding (PF13460; HMM-score: 11.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.029732
  • TAT(Tat/SPI): 0.000589
  • LIPO(Sec/SPII): 0.005143
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160579 NCBI
• RefSeq: YP_493454 NCBI
• UniProt: Q2FIM4 UniProt

⊟Protein sequence

• MKQYLITGGTGMVGSQLVNEIKKSDSHITILTRHDQISNDKKISYVNWAKSGWEHKVPQNIDVVINLAGATLNKRWTPEYKQTLMLSRIQSTQALYELFKSRNKAPKALFNASATGYYPPDLFMSYTEVYKTLPFDFLSDIVYQWERFAQQFEQLGTRVVIGRFGIILSNEGGALQTMKLPYEYYIGGKLGSGQQWYSWIHINDLIQAILFLINNESASGPFNLTAPIPERQNLFGYTLARAMHKPHETWAPSLAMRLILGQMSTVVLDTQKVLPNKIQALGFQFKYSNLKMALEDLIKE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• data available for COL, NCTC8325, Newman

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications