PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0721 [new locus tag: SAUSA300_RS03880 ]
• pan locus tag^?: SAUPAN002640000
• symbol: SAUSA300_0721
• pan gene symbol^?: sstD
• synonym:
• product: transferrin receptor

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0721 [new locus tag: SAUSA300_RS03880 ]
• symbol: SAUSA300_0721
• product: transferrin receptor
• replicon: chromosome
• strand: +
• coordinates: 800827..801855
• length: 1029
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3915038 NCBI
• RefSeq: YP_493424 NCBI
• BioCyc: see SAUSA300_RS03880
• MicrobesOnline: 1292236 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  1021
  
  ATGAAGAAAACAGTCTTATATTTAGTATTAGCAGTAATGTTTTTATTAGCGGCATGCGGT
  AACAATTCTGATAAAGAACAATCAAAATCAGAAACTAAAGGTTCTAAAGATACAGTAAAA
  ATTGAAAATAACTATAAAATGCGTGGCGAGAAAAAAGATGGTAGTGACGCTAAAAAAGTT
  AAAGAAACTGTTGAAGTACCAAAAAATCCTAAAAATGCAGTTGTGTTAGACTATGGCGCA
  TTAGATGTAATGAAAGAAATGGGCTTATCAGATAAAGTAAAAGCATTACCTAAAGGGGAA
  GGCGGTAAGTCATTACCGAATTTCTTAGAATCATTTAAAGATGATAAATATACAAACGTT
  GGTAATTTAAAAGAAGTGAATTTTGATAAAATTGCTGCGACGAAACCCGAAGTAATCTTT
  ATCTCTGGACGTACAGCTAATCAAAAGAATTTAGATGAATTCAAAAAAGCTGCACCTAAA
  GCGAAAATTGTTTATGTTGGTGCAGATGAAAAGAACTTAATTGGTTCAATGAAACAAAAC
  ACTGAAAATATCGGAAAAATTTACGATAAAGAAGATAAAGCTAAAGAATTAAATAAAGAT
  TTAGATAACAAAATTGCTTCAATGAAAGATAAAACGAAAAACTTCAATAAAACTGTTATG
  TATTTACTAGTTAACGAAGGTGAATTATCAACATTTGGACCTAAAGGTCGTTTTGGTGGA
  TTAGTTTACGATACATTAGGATTCAATGCAGTTGATAAAAAAGTAAGTAATAGCAATCAT
  GGACAAAATGTTTCTAACGAATATGTTAATAAAGAAAATCCAGATGTTATTTTAGCGATG
  GATAGAGGTCAAGCGATAAGTGGTAAATCAACTGCGAAACAAGCATTAAATAATCCTGTA
  TTAAAAAATGTTAAAGCAATTAAAGAAGACAAAGTATATAATTTAGATCCTAAATTATGG
  TACTTTGCAGCTGGATCAACTACAACTACAATTAAACAAATTGAGGAACTTGATAAAGTT
  GTAAAATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_0721 [new locus tag: SAUSA300_RS03880 ]
• symbol: SAUSA300_0721
• description: transferrin receptor
• length: 342
• theoretical pI: 9.92882
• theoretical MW: 37854.1
• GRAVY: -0.652632

⊟Function

• TIGRFAM:
  heme ABC transporter, heme-binding protein isdE (TIGR03659; HMM-score: 76.6)
  and 3 more
  putative ABC transporter PGF-CTERM-modified substrate-binding protein (TIGR04281; HMM-score: 50.6)
  proposed F420-0 ABC transporter, periplasmic F420-0 binding protein (TIGR03868; HMM-score: 23.9)
  Cellular processes Cellular processes Sporulation and germination sporulation lipoprotein, YhcN/YlaJ family (TIGR02898; HMM-score: 15.6)
• TheSEED  :
  • Iron compound ABC uptake transporter substrate-binding protein
  Iron acquisition and metabolism Iron acquisition and metabolism - no subcategory Heme, hemin uptake and utilization systems in GramPositives  Iron compound ABC uptake transporter substrate-binding protein
  and 1 more
  Iron acquisition and metabolism Iron acquisition and metabolism - no subcategory Iron acquisition in Streptococcus  Iron compound ABC uptake transporter substrate-binding protein
• PFAM:
  Chelatase (CL0043) Peripla_BP_2; Periplasmic binding protein (PF01497; HMM-score: 107.4)
  and 1 more
  no clan defined Formyl_trans_N; Formyl transferase (PF00551; HMM-score: 13.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 9.68
  • Cellwall Score: 0.17
  • Extracellular Score: 0.16
  • Internal Helix: 1
• LocateP: Lipid anchored
  • Prediction by SwissProt Classification: Extracellular
  • Pathway Prediction: Sec-(SPII)
  • Intracellular possibility: 0
  • Signal peptide possibility: 0.5
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: FLLAACG
• SignalP: Signal peptide LIPO(Sec/SPII) length 18 aa
  • SP(Sec/SPI): 0.000351
  • TAT(Tat/SPI): 0.000056
  • LIPO(Sec/SPII): 0.999451
  • Cleavage Site: CS pos: 18-19. LAA-CG. Pr: 0.9999
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87162442 NCBI
• RefSeq: YP_493424 NCBI
• UniProt: A0A0H2XKM7 UniProt

⊟Protein sequence

• MKKTVLYLVLAVMFLLAACGNNSDKEQSKSETKGSKDTVKIENNYKMRGEKKDGSDAKKVKETVEVPKNPKNAVVLDYGALDVMKEMGLSDKVKALPKGEGGKSLPNFLESFKDDKYTNVGNLKEVNFDKIAATKPEVIFISGRTANQKNLDEFKKAAPKAKIVYVGADEKNLIGSMKQNTENIGKIYDKEDKAKELNKDLDNKIASMKDKTKNFNKTVMYLLVNEGELSTFGPKGRFGGLVYDTLGFNAVDKKVSNSNHGQNVSNEYVNKENPDVILAMDRGQAISGKSTAKQALNNPVLKNVKAIKEDKVYNLDPKLWYFAAGSTTTTIKQIEELDKVVK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0718 > SAUSA300_0719 > SAUSA300_0720 > SAUSA300_0721

⊟Regulation

• regulator: Fur* (repression) regulon

  Fur*

  (TF)

  important in Iron homeostasis; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications