From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0693 [new locus tag: SAUSA300_RS03720 ]
  • pan locus tag?: SAUPAN002594000
  • symbol: SAUSA300_0693
  • pan gene symbol?: saeP
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0693 [new locus tag: SAUSA300_RS03720 ]
  • symbol: SAUSA300_0693
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 768717..769157
  • length: 441
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGAATACAAAATATTTTTTAGCAGCTGGTGCTGTTATCACAACATTAGCTTTAGGTGCT
    TGTGGTAATTCTAATTCACAAGATCAAGGTAACAAAACTGAACAAAAAACAAAGTCAGAA
    GATAGCAATGTTAAAACTGATAAAACAAAACACCTAACAGGTACATTCAGTTCTAAAAAC
    GGTGAAACTGTTGAAGGTAAAGCTGAGATTAAAAATGGTAAATTAATGCTTACTAACTAC
    AAATCATCAAAAGGTCCAGATTTATACGTCTACCTAACAAAAAATGGCGACATTAAAAAC
    GGTAAAGAAATCGCAATGGTTGACTACGATAAAGAAAAACAAACATTTGATCTTAAAAAT
    GTAGATTTAAGCAAATATGATGAAGTAACAATCTATTGTAAAAAAGCTCACGTCATCTTC
    GGCGGCGCTAAATTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    441

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_0693 [new locus tag: SAUSA300_RS03720 ]
  • symbol: SAUSA300_0693
  • description: hypothetical protein
  • length: 146
  • theoretical pI: 9.62012
  • theoretical MW: 16045.1
  • GRAVY: -0.739041

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • FIG01107853: hypothetical protein
  • PFAM:
    no clan defined DM13; Electron transfer DM13 (PF10517; HMM-score: 41.9)
    and 3 more
    DUF1797; Protein of unknown function (DUF1797) (PF08796; HMM-score: 16)
    YcgL; YcgL domain (PF05166; HMM-score: 12.6)
    CfAFP; Choristoneura fumiferana antifreeze protein (CfAFP) (PF05264; HMM-score: 12.2)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 3.33
    • Cellwall Score: 3.33
    • Extracellular Score: 3.33
    • Internal Helix: 1
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular possibility: -0.33
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: LALGACG
  • SignalP: Signal peptide LIPO(Sec/SPII) length 20 aa
    • SP(Sec/SPI): 0.00076
    • TAT(Tat/SPI): 0.00011
    • LIPO(Sec/SPII): 0.998886
    • Cleavage Site: CS pos: 20-21. LGA-CG. Pr: 0.9994
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MNTKYFLAAGAVITTLALGACGNSNSQDQGNKTEQKTKSEDSNVKTDKTKHLTGTFSSKNGETVEGKAEIKNGKLMLTNYKSSKGPDLYVYLTKNGDIKNGKEIAMVDYDKEKQTFDLKNVDLSKYDEVTIYCKKAHVIFGGAKLK

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator: SaeR (activation) regulon
    SaeR(TF)important in Virulence; RegPrecise 

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]