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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0655 [new locus tag: SAUSA300_RS03510 ]
  • pan locus tag?: SAUPAN002549000
  • symbol: SAUSA300_0655
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0655 [new locus tag: SAUSA300_RS03510 ]
  • symbol: SAUSA300_0655
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 731805..732521
  • length: 717
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGGGACGTAAATGGAATAACATTAAAGAAAAAAAGGCCCAAAAAGATAAAAACACAAGT
    AGAATATATGCGAAATTTGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAAT
    CCAGAATCTAACCAAGCTTTAAGGTTGGTGCTTGAACGCGCTAAGACATATTCAGTGCCG
    AATCATATTATTGAAAAAGCAATAGATAAAGCTAAGGGTGCTGGAGACGAAAACTTTGAT
    CACCTAAGATATGAAGGATTTGGCCCAAGCGGATCAATGCTAATTGTTGATGCGTTAACA
    AATAATGTAAATCGTACTGCCTCTGATGTGCGAGCTGCTTTTGGTAAAAACGGCGGTAAT
    ATGGGTGTATCTGGATCAGTTGCTTATATGTTTGATCATGTGGCAACATTTGGTATTGAA
    GGAAAGTCTGTTGACGAAATACTTGAAACATTAATGGAACAAGATGTAGATGTAAATGAT
    GTGATTGACGATAATGGATTGACAATAGTCTATGCTGAACCAGATCAATTTGCAGTCGTT
    CAAGATGCGCTTCGTGCAGCAGGTGTTGAAGAATTTAAAGTTGCTGAATTTGAAATGTTA
    CCTCAAACAGATATTGAACTTTCTGAAGCGGACCAAGTAACATTTGAAAAATTAATCGAT
    GCATTAGAAGATTTAGAAGATGTACAAAACGTATTCCATAATGTGGATTTGAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    717

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_0655 [new locus tag: SAUSA300_RS03510 ]
  • symbol: SAUSA300_0655
  • description: hypothetical protein
  • length: 238
  • theoretical pI: 4.37556
  • theoretical MW: 26320.2
  • GRAVY: -0.413445

Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions DNA-binding regulatory protein, YebC/PmpR family (TIGR01033; HMM-score: 227.2)
    and 1 more
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type VII secretion protein EccB (TIGR03919; HMM-score: 11)
  • TheSEED  :
    • Transcriptional regulatory protein YeeN
  • PFAM:
    no clan defined Transcrip_reg; Transcriptional regulator (PF01709; HMM-score: 264.8)
    and 2 more
    GatB_YqeY (CL0279) GatB_Yqey; GatB domain (PF02637; HMM-score: 14.1)
    Ank (CL0465) Ank_4; Ankyrin repeats (many copies) (PF13637; HMM-score: 12.4)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.01061
    • TAT(Tat/SPI): 0.000749
    • LIPO(Sec/SPII): 0.002688
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MGRKWNNIKEKKAQKDKNTSRIYAKFGKEIYVAAKSGEPNPESNQALRLVLERAKTYSVPNHIIEKAIDKAKGAGDENFDHLRYEGFGPSGSMLIVDALTNNVNRTASDVRAAFGKNGGNMGVSGSVAYMFDHVATFGIEGKSVDEILETLMEQDVDVNDVIDDNGLTIVYAEPDQFAVVQDALRAAGVEEFKVAEFEMLPQTDIELSEADQVTFEKLIDALEDLEDVQNVFHNVDLK

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]