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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0493 [new locus tag: SAUSA300_RS02645 ]
  • pan locus tag?: SAUPAN002257000
  • symbol: folB
  • pan gene symbol?: folB
  • synonym:
  • product: dihydroneopterin aldolase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0493 [new locus tag: SAUSA300_RS02645 ]
  • symbol: folB
  • product: dihydroneopterin aldolase
  • replicon: chromosome
  • strand: +
  • coordinates: 552775..553140
  • length: 366
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    ATGCAAGACACAATCTTTCTTAAAGGTATGCGCTTTTATGGATATCATGGTGCTTTATCA
    GCTGAAAATGAAATAGGGCAAATTTTCAAAGTGGATGTAACTTTGAAAGTAGACTTAGCT
    GAAGCTGGGCGTACTGATAATGTTATTGATACAGTTCATTATGGTGAAGTGTTCGAAGAG
    GTTAAATCAATTATGGAAGGTAAGGCCGTTAATTTACTTGAGCATCTAGCTGAACGTATT
    GCAAATCGTATAAATTCACAATATAATCGTGTAATGGAAACGAAAGTGAGAATCACTAAA
    GAAAACCCACCGATTCCGGGTCATTATGATGGAGTAGGTATCGAAATAGTGAGGGAGAAT
    AAATGA
    60
    120
    180
    240
    300
    360
    366

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_0493 [new locus tag: SAUSA300_RS02645 ]
  • symbol: FolB
  • description: dihydroneopterin aldolase
  • length: 121
  • theoretical pI: 5.63716
  • theoretical MW: 13734.5
  • GRAVY: -0.367769

⊟Function[edit | edit source]

  • reaction:
    EC 4.1.2.25?  ExPASy
    Dihydroneopterin aldolase 7,8-dihydroneopterin = 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Folic acid dihydroneopterin aldolase (TIGR00525; EC 4.1.2.25; HMM-score: 126.6)
    FolB domain (TIGR00526; HMM-score: 104.8)
  • TheSEED  :
    • 7,8-dihydroneopterin epimerase (EC 5.1.99.8)
    • Dihydroneopterin aldolase (EC 4.1.2.25)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Folate and pterines Folate Biosynthesis  Dihydroneopterin aldolase (EC 4.1.2.25)
  • PFAM:
    THBO-biosyn (CL0334) FolB; Dihydroneopterin aldolase (PF02152; HMM-score: 111)
    and 1 more
    no clan defined Cactin_mid; Conserved mid region of cactin (PF10312; HMM-score: 12)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006459
    • TAT(Tat/SPI): 0.000373
    • LIPO(Sec/SPII): 0.000548
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MQDTIFLKGMRFYGYHGALSAENEIGQIFKVDVTLKVDLAEAGRTDNVIDTVHYGEVFEEVKSIMEGKAVNLLEHLAERIANRINSQYNRVMETKVRITKENPPIPGHYDGVGIEIVRENK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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