PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0327 [new locus tag: SAUSA300_RS01740 ]
• pan locus tag^?: SAUPAN001858000
• symbol: SAUSA300_0327
• pan gene symbol^?: sirTM
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0327 [new locus tag: SAUSA300_RS01740 ]
• symbol: SAUSA300_0327
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 379288..380232
• length: 945
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913647 NCBI
• RefSeq: YP_493041 NCBI
• BioCyc: see SAUSA300_RS01740
• MicrobesOnline: 1291842 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  901
  
  ATGCAGAGTAGTAAGTGGAATGCAATGTCTCTGTTAATGGATGACAAGACAAAGCAGGCT
  GAAGTATTGCGTACTGCGATTGATGAAGCAGATGCGATAGTGATTGGAATTGGTGCAGGC
  ATGTCTGCATCTGACGGATTTACATATGTAGGAGAGCGTTTTACGGAAAATTTCCCAGAT
  TTTATTGAAAAATATCGCTTCTTTGATATGTTGCAAGCGAGTTTACATCCTTATGGCAGT
  TGGCAAGAGTATTGGGCATTTGAGAGTCGTTTTATTACATTAAACTATTTAGATCAACCT
  GTAGGTCAGTCTTACCTCGCTTTAAAATCCTTGGTGGAAGGTAAACAGTACCACATTATA
  ACTACGAATGCAGATAATGCTTTCGATGTAGCTGATTATGATATGACTCATGTATTTCAT
  ATACAAGGGGAGTATATACTGCAACAGTGTAGTCAGCATTGTCATGCTCAAACGTATCGC
  AATGATGATTTAATTCGTAAAATGGTTGTTGCGCAACAAGATATGCTTATACCTTGGGAG
  ATGATTCCAAGATGTCCAAAATGTGATGCCCCAATGGAAGTGAATAAACGTAAAGCGGAA
  GTTGGGATGGTTGAAGATGCTGAATTTCATGCGCAACTACATCGTTATAATGCTTTTCTA
  GAGCAACATCAAGATGATAAAGTGTTGTATTTGGAAATTGGAATTGGTTATACTACACCA
  CAATTTGTGAAGCATCCTTTTCAGCGTATGACACGTAAAAATGAAAATGCCCTTTATATG
  ACGATGAATAAAAAGGCATATCGCATTCCGAATTCAATTCAAGAACGTACCATACATTTA
  ACTGAGGATATCTCAACATTGATTACAGCAGCACTCCGGAACGACAGCACAACGAAAAAT
  AACAACATTGGAGAGACAGAAGATGTACTTAATAGAACCGATTAG
  60
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  945
  

⊟Protein

⊟General

• locus tag: SAUSA300_0327 [new locus tag: SAUSA300_RS01740 ]
• symbol: SAUSA300_0327
• description: hypothetical protein
• length: 314
• theoretical pI: 5.2205
• theoretical MW: 36396.9
• GRAVY: -0.505414

⊟Function

• TIGRFAM:
  molybdenum cofactor synthesis domain (TIGR00177; HMM-score: 15.8)
• TheSEED  :
  • NAD-dependent protein deacetylase of SIR2 family
  Carbohydrates Central carbohydrate metabolism Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate  NAD-dependent protein deacetylase of SIR2 family
  and 1 more
  Stress Response Oxidative stress Redox-dependent regulation of nucleus processes  NAD-dependent protein deacetylase of SIR2 family
• PFAM:

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9915
  • Cytoplasmic Membrane Score: 0.0021
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0065
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.009318
  • TAT(Tat/SPI): 0.000573
  • LIPO(Sec/SPII): 0.001271
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161578 NCBI
• RefSeq: YP_493041 NCBI
• UniProt: A0A0H2XHK5 UniProt

⊟Protein sequence

• MQSSKWNAMSLLMDDKTKQAEVLRTAIDEADAIVIGIGAGMSASDGFTYVGERFTENFPDFIEKYRFFDMLQASLHPYGSWQEYWAFESRFITLNYLDQPVGQSYLALKSLVEGKQYHIITTNADNAFDVADYDMTHVFHIQGEYILQQCSQHCHAQTYRNDDLIRKMVVAQQDMLIPWEMIPRCPKCDAPMEVNKRKAEVGMVEDAEFHAQLHRYNAFLEQHQDDKVLYLEIGIGYTTPQFVKHPFQRMTRKNENALYMTMNKKAYRIPNSIQERTIHLTEDISTLITAALRNDSTTKNNNIGETEDVLNRTD

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0324 > SAUSA300_0325 > SAUSA300_0326 > SAUSA300_0327 > SAUSA300_0328

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications

Johannes Gregor Matthias Rack, Rosa Morra, Eva Barkauskaite, Rolf Kraehenbuehl, Antonio Ariza, Yue Qu, Mary Ortmayer, Orsolya Leidecker, David R Cameron, Ivan Matic, Anton Y Peleg, David Leys, Ana Traven, Ivan Ahel
Identification of a Class of Protein ADP-Ribosylating Sirtuins in Microbial Pathogens.
Mol Cell: 2015, 59(2);309-20
[PubMed:26166706] [WorldCat.org] [DOI] (I p)