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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0215 [new locus tag: SAUSA300_RS01130 ]
  • pan locus tag?: SAUPAN001073000
  • symbol: SAUSA300_0215
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0215 [new locus tag: SAUSA300_RS01130 ]
  • symbol: SAUSA300_0215
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 254254..254826
  • length: 573
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    TTGACAAGCTTTATCTATAAAATACTTTATGTTGTCAAAATCAACGCTTATACATATGAC
    ATAATGACGGAGGACATCATGATTCTATCTATATTACTTATCTTTTTCTGTATCAGACTT
    GTCAGCTTAAAGATATCTATTAATCACTCAAAACAATTAAAAGCAGACGGTGCAGTTGAA
    TATGGCGTTAAAAATTCGAAGTTTTTAGCAATAACACACGTTTTAATTTATGTATTGGCT
    GGTGTAGAGGCATTTATTAATAAAGATACATTTAGCTTTGCAAATGGTATTGGCTTAGTT
    ATATTAATCTTTGCTTATATCATGTTATTTATGGTTATTAAAACTTTAGGTGGTATTTGG
    ACATTAAAATTATTCATTTTACCTAACCATCCTATTATTAAATCTGGATTATATAAAATT
    ACAAAACACCCAAATTACTTCTTAAACATCATTCCTGAATTAATCGGTGTATTATTATTA
    ACACACGCAACATATACAACTATTTTATTAGTACCGTATGCGTATTTCTTATATGTACGT
    ATTAAACAAGAAGAGAAATTAATGAATATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    573

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_0215 [new locus tag: SAUSA300_RS01130 ]
  • symbol: SAUSA300_0215
  • description: hypothetical protein
  • length: 190
  • theoretical pI: 9.73661
  • theoretical MW: 21779.2
  • GRAVY: 0.823684

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • FIG01107841: hypothetical protein
  • PFAM:
    Steroid_dh (CL0115) ICMT; Isoprenylcysteine carboxyl methyltransferase (ICMT) family (PF04140; HMM-score: 105.7)
    and 3 more
    PEMT; Phospholipid methyltransferase (PF04191; HMM-score: 25.6)
    DUF1295; Protein of unknown function (DUF1295) (PF06966; HMM-score: 17.9)
    Transthyretin (CL0287) DUF2606; Protein of unknown function (DUF2606) (PF10794; HMM-score: 12.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 3
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003899
    • TAT(Tat/SPI): 0.000204
    • LIPO(Sec/SPII): 0.000753
  • predicted transmembrane helices (TMHMM): 4

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTSFIYKILYVVKINAYTYDIMTEDIMILSILLIFFCIRLVSLKISINHSKQLKADGAVEYGVKNSKFLAITHVLIYVLAGVEAFINKDTFSFANGIGLVILIFAYIMLFMVIKTLGGIWTLKLFILPNHPIIKSGLYKITKHPNYFLNIIPELIGVLLLTHATYTTILLVPYAYFLYVRIKQEEKLMNI

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SaeR (activation) regulon
    SaeR(TF)important in Virulence; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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