PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0188 [new locus tag: SAUSA300_RS00985 ]
• pan locus tag^?: SAUPAN001028000
• symbol: brnQ
• pan gene symbol^?: brnQ1
• synonym:
• product: branched-chain amino acid transport system II carrier protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0188 [new locus tag: SAUSA300_RS00985 ]
• symbol: brnQ
• product: branched-chain amino acid transport system II carrier protein
• replicon: chromosome
• strand: -
• coordinates: 217164..218519
• length: 1356
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913551 NCBI
• RefSeq: YP_492902 NCBI
• BioCyc: see SAUSA300_RS00985
• MicrobesOnline: 1291703 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGAAGAAGAAACTAACATTTAAAGAAAACATGTTTATAGGTTCTATGTTATTTGGTTTA
  TTCTTTGGTGCCGGCAATCTTATCTTCCCAATACACTTGGGTCAAGCTGCTGGTTCTAAC
  GTTTTTATCGCTAACTTAGGATTTTTAATTACAGCAATTGGCTTACCATTTCTAGGTATC
  ATTGCTATTGGCATTTCAAAGACATCTGGTTTATTTGAAATTGCATCGCGTGTTAATAAA
  ACATATGCTTACATTTTCACGATTGCCTTATATCTAGTTATCGGACCATTTTTCGCCTTA
  CCTAGACTGGCAACGACATCATTTGAAATTGCATTTTCGCCATTTTTATCACCAAAGCAA
  ATCACTTTATATTTATTTATTTTTAGCTTCGTCTTCTTTGTGATTGCATGGTTTTTTGCG
  AGAAAGCCATCAAGAATTTTAGAATATATCGGTAAATTTTTAAATCCGGTATTCTTAGTA
  TTATTAGCAATTATTTTATTATTTGCTTTTATCCATCCATTAGGTGGCATATCTGATGCA
  CCTATTAGTAAACAATATCAATCACATGCCTTATTTAACGGCTTTTTAGATGGATACAAT
  ACCTTAGATGCGCTAGCGTCATTGGCATTTGGTATTATCATTGTTGCAACGATTAAAAAG
  TTAGGTATCGAAAATCCAACTGATATCGCTAAAGAAACAATTAAGTCTGGTACTATCAGT
  ATCATTATGATGGGGATCATTTATACCCTACTAGCAATCATGGGTACATTAAGTATTGGT
  CATTTCAAACTTAGTGAAAATGGTGGTATTGCCTTAGCGCAAATTACTCAATACTACTTA
  GGTAACTACGGTATCGTCCTGTTGTCACTTATCGTTATGGTTGCTTGTTTAAAAACAGCC
  ATCGGTTTGATTACGGCATTTTCAGAAACATTCGAACACCTTTTCCCTAAAATGAATTAC
  CTAGCGATTGCAACAGTTGTAAGCTTTATTTCGTTCTTATTCGCGAATGTTGGTTTAACT
  AAGATTATTATGTACTCAGTCCCAGTGTTAATGTTCTTATATCCATTAGCAATTGCCTTG
  ATTGTACTAACATTATTTAGTAGCAAATTCCATCATTCAAAACTTATTTATCAATGTACC
  ATTTTCTTTACAATGATTGCTGCATTAGTAGATGGATTAAAAGCTAGTCCAGAGTTCATT
  TCAAGCACATCATTCTCACAAACTTTGATTAATTTCAGCCAAAAATATTTACCATTATCA
  GACATTGGTATGGGCTGGGTTGTTCTCAGTTTGATTGGTTTCATTATCGGCTTCATTATT
  TATAAAATTAAGCATCGTAAAATTCCACAAGCATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_0188 [new locus tag: SAUSA300_RS00985 ]
• symbol: BrnQ
• description: branched-chain amino acid transport system II carrier protein
• length: 451
• theoretical pI: 10.0084
• theoretical MW: 49700.4
• GRAVY: 0.958093

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Amino acids, peptides and amines branched-chain amino acid transport system II carrier protein (TIGR00796; HMM-score: 412.2)
• TheSEED  :
  • Branched-chain amino acid transport system carrier protein
• PFAM:
  APC (CL0062) Branch_AA_trans; Branched-chain amino acid transport protein (PF05525; HMM-score: 534.2)
  and 1 more
  no clan defined Renin_r; Renin receptor-like protein (PF07850; HMM-score: 10.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 12
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -0.5
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.095231
  • TAT(Tat/SPI): 0.002887
  • LIPO(Sec/SPII): 0.046472
• predicted transmembrane helices (TMHMM): 12

⊟Accession numbers

• GI: 87160830 NCBI
• RefSeq: YP_492902 NCBI
• UniProt: A0A0H2XHJ7 UniProt

⊟Protein sequence

• MKKKLTFKENMFIGSMLFGLFFGAGNLIFPIHLGQAAGSNVFIANLGFLITAIGLPFLGIIAIGISKTSGLFEIASRVNKTYAYIFTIALYLVIGPFFALPRLATTSFEIAFSPFLSPKQITLYLFIFSFVFFVIAWFFARKPSRILEYIGKFLNPVFLVLLAIILLFAFIHPLGGISDAPISKQYQSHALFNGFLDGYNTLDALASLAFGIIIVATIKKLGIENPTDIAKETIKSGTISIIMMGIIYTLLAIMGTLSIGHFKLSENGGIALAQITQYYLGNYGIVLLSLIVMVACLKTAIGLITAFSETFEHLFPKMNYLAIATVVSFISFLFANVGLTKIIMYSVPVLMFLYPLAIALIVLTLFSSKFHHSKLIYQCTIFFTMIAALVDGLKASPEFISSTSFSQTLINFSQKYLPLSDIGMGWVVLSLIGFIIGFIIYKIKHRKIPQA

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator: CodY (repression) regulon

  CodY

  (TF)

  important in Amino acid metabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications