PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0138 [new locus tag: SAUSA300_RS00725 ]
• pan locus tag^?: SAUPAN000948000
• symbol: deoD
• pan gene symbol^?: deoD1
• synonym:
• product: purine nucleoside phosphorylase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0138 [new locus tag: SAUSA300_RS00725 ]
• symbol: deoD
• product: purine nucleoside phosphorylase
• replicon: chromosome
• strand: +
• coordinates: 157077..157784
• length: 708
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913848 NCBI
• RefSeq: YP_492855 NCBI
• BioCyc: see SAUSA300_RS00725
• MicrobesOnline: 1291653 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAAATCAACACCACACATTAAACCAATGAATGACGTCGAAATTGCAGAAACGGTTCTA
  TTGCCAGGAGATCCGTTAAGAGCTAAGTTCATTGCAGAAACTTATTTGGATGATGTGGAA
  CAGTTCAATACAGTGCGAAACATGTTTGGTTTTACCGGAACATATAAAGGTAAAAAAGTT
  TCTGTCATGGGTTCAGGTATGGGTATGCCATCTATTGGCATTTACTCTTATGAATTAATT
  CATACATTTGGTTGTAAAAAATTAATTCGCGTTGGCTCTTGTGGCGCGATGCAAGAAAAC
  ATTGATTTATATGATGTGATTATTGCACAAGGTGCCTCTACTGATTCAAATTACGTTCAA
  CAATATCAATTACCAGGTCATTTTGCGCCAATTGCTTCTTATCAATTATTAGAAAAAGCA
  GTTGAAACAGCACGTGACAAAGGTGTACGTCATCATGTAGGTAATGTGTTATCAAGTGAT
  ATTTTCTATAACGCGGATACAACAGCGAGTGAACGTTGGATGCGTATGGGTATTTTAGGT
  GTAGAAATGGAATCAGCTGCATTATACATGAATGCAATTTACGCTGGTGTCGAAGCATTA
  GGTGTGTTCACAGTGAGCGATCATTTAATTCATGAAACGTCAACAACACCTGAGGAAAGG
  GAACGTGCATTTACAGATATGATTGAAATTGCACTGTCATTGGTGTAG
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⊟Protein

⊟General

• locus tag: SAUSA300_0138 [new locus tag: SAUSA300_RS00725 ]
• symbol: DeoD
• description: purine nucleoside phosphorylase
• length: 235
• theoretical pI: 4.9407
• theoretical MW: 25991.5
• GRAVY: -0.0493617

⊟Function

• reaction:
  EC 2.4.2.1?  ExPASy
  Purine-nucleoside phosphorylase Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
• TIGRFAM:
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides purine nucleoside phosphorylase (TIGR00107; EC 2.4.2.1; HMM-score: 297.1)
  and 7 more
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides uridine phosphorylase (TIGR01718; EC 2.4.2.3; HMM-score: 117.2)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides MTA/SAH nucleosidase (TIGR01704; EC 3.2.2.16,3.2.2.9; HMM-score: 30.2)
  Metabolism Central intermediary metabolism Other MTA/SAH nucleosidase (TIGR01704; EC 3.2.2.16,3.2.2.9; HMM-score: 30.2)
  futalosine hydrolase (TIGR03664; EC 3.2.2.26; HMM-score: 29.5)
  hopanoid-associated phosphorylase (TIGR03468; HMM-score: 25.6)
  putative AMP nucleosidase (TIGR01721; HMM-score: 24)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides uridine phosphorylase (TIGR01719; EC 2.4.2.3; HMM-score: 20.5)
• TheSEED  :
  • Purine nucleoside phosphorylase (EC 2.4.2.1)
  Carbohydrates Monosaccharides Deoxyribose and Deoxynucleoside Catabolism  Purine nucleoside phosphorylase (EC 2.4.2.1)
  and 2 more
  Nucleosides and Nucleotides Purines Purine conversions  Purine nucleoside phosphorylase (EC 2.4.2.1)
  Nucleosides and Nucleotides Pyrimidines pyrimidine conversions  Purine nucleoside phosphorylase (EC 2.4.2.1)
• PFAM:
  PUP (CL0408) PNP_UDP_1; Phosphorylase superfamily (PF01048; HMM-score: 136)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.017472
  • TAT(Tat/SPI): 0.000888
  • LIPO(Sec/SPII): 0.000873
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87159961 NCBI
• RefSeq: YP_492855 NCBI
• UniProt: A0A0H2XDJ1 UniProt

⊟Protein sequence

• MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNTVRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENIDLYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVETARDKGVRHHVGNVLSSDIFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPEERERAFTDMIEIALSLV

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: deoD > SAUSA300_0139

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications