⊟Summary[edit | edit source]
- pan ID?: SAUPAN004214000
- symbol?: —
- synonym:
- description?: IreB family regulatory phosphoprotein
- IreB family regulatory phosphoprotein
- hypothetical protein with DUF935
- protein Stu1959
descriptions from strain specific annotations:
- strand?: -
- coordinates?: 4464421..4464681
- synteny block?: BlockID0032420
- occurrence?: in 100% of 34 strains
reoM : regulator of MurA degradation ReoY-binding regulatory phosphoprotein [1]
The ReoMY system regulates ClpCP-mediated degradation of MurA, the enzyme catalyzing the first committed step of peptidoglycan biosynthesis. The Stk1/Stp1 serine-threonine kinase/phosphatase system controls the phosphorylation state of ReoM ; dephosphorylated ReoM binds to both MurA and ReoY. ReoY is a ClpCP-binding adaptor protein that targets ReoM's MurA cargo for proteolysis when ReoM is in the dephosphorylated state. When PG synthesis is required, Stk1 phosphorylates ReoM, MurA no longer binds ReoY, MurA is no longer targeted for proteolysis and the rate of cell wall synthesis increases. The alternative UDP-NAG carboxyvinyltransferase MurZ also plays a currently undefined role in targeting MurA for ClpCP-mediated proteolysis.
⊟Orthologs[edit | edit source]
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MENFDKTMKFDYEELPTQDVRDVLNNVYRTLDERGYNAVNQIVGYLLSGDPAYIPRQNEA
N315 MENFDKTMKFDYEELPTQDVRDVLNNVYRTLDERGYNAVNQIVGYLLSGDPAYIPRQNEA
NCTC8325 MENFDKTMKFDYEELPTQDVRDVLNNVYRTLDERGYNAVNQIVGYLLSGDPAYIPRQNEA
Newman MENFDKTMKFDYEELPTQDVRDVLNNVYRTLDERGYNAVNQIVGYLLSGDPAYIPRQNEA
USA300_FPR3757 MENFDKTMKFDYEELPTQDVRDVLNNVYRTLDERGYNAVNQIVGYLLSGDPAYIPRQNEA
************************************************************
COL RNQIRHIDRDVIMEELVSYYLKEQNK
N315 RNQIRHIDRDVIMEELVSYYLKEQNK
NCTC8325 RNQIRHIDRDVIMEELVSYYLKEQNK
Newman RNQIRHIDRDVIMEELVSYYLKEQNK
USA300_FPR3757 RNQIRHIDRDVIMEELVSYYLKEQNK
**************************
- ↑ Jessica L Kelliher, Caroline M Grunenwald, Rhiannon R Abrahams, McKenzie E Daanen, Cassandra I Lew, Warren E Rose, John-Demian Sauer
PASTA kinase-dependent control of peptidoglycan synthesis via ReoM is required for cell wall stress responses, cytosolic survival, and virulence in Listeria monocytogenes.
PLoS Pathog: 2021, 17(10);e1009881
[PubMed:34624065] [WorldCat.org] [DOI] (I e)Sabrina Wamp, Patricia Rothe, Daniel Stern, Gudrun Holland, Janina Döhling, Sven Halbedel
MurA escape mutations uncouple peptidoglycan biosynthesis from PrkA signaling.
PLoS Pathog: 2022, 18(3);e1010406
[PubMed:35294506] [WorldCat.org] [DOI] (I e)Malcolm E Winkler, Merrin Joseph, Ho-Ching T Tsui
Secrets of getting started: Regulation of the first committed step of peptidoglycan synthesis by protein phosphorylation in Enterococcus and other Gram-positive bacteria.
Mol Microbiol: 2023, 120(6);805-810
[PubMed:38012814] [WorldCat.org] [DOI] (I p)