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⊟Summary[edit | edit source]
- pan ID?: SAUPAN004073000
- symbol?: lpdA
- synonym:
- description?: dihydrolipoyl dehydrogenase
- dihydrolipoyl dehydrogenase
- dihydrolipoamide dehydrogenase
- 2-oxoisovalerate dehydrogenase, E3 component, lipoamide dehydrogenase
- Dihydrolipoamide dehydrogenase of branched-chain alpha-keto acid dehydrogenase
- putative dihydrolipoamide dehydrogenase
descriptions from strain specific annotations:
- strand?: -
- coordinates?: 4354449..4355870
- synteny block?: BlockID0031050
- occurrence?: in 100% of 34 strains
lpdA : branched-chain alpha-keto acid dehydrogenase E3 (dihydrolipoamide dehydrogenase) subunit [1]
To maintain or modulate their membrane fluidity, staphylococci catabolize deaminated branched-chain amino acids and use the Branched-Chain Alpha-Keto Acid Dehydrogenase (BCAKAD) complex to immobilize them as branched-chain Coenzyme A thioesters for subsequent modification into branched-chain fatty acids. The E1 heterodimer decarboxylates and thioesterifies BCAKAs to the lipoylated-E2 subunit, then the E2 subunit (transacylase) converts the BCAKA-dihydrolipoyl thioester to a BCAKA-CoA thioester and releases the product. Finally, the E3 subunit reduces the dehydrolipoylated-E2 subunit regenerating a functional lipoylated-E2.
⊟Orthologs[edit | edit source]
04-02981:
SA2981_1477
08BA02176:
C248_1561 (bfmBC)
11819-97:
MS7_1536
6850:
RSAU_001385 (lpdA)
71193:
ST398NM01_1584
ECT-R 2:
ECTR2_1370 (lpdA)
ED133:
SAOV_1519 (lpdA)
ED98:
SAAV_1511 (lpdA)
HO 5096 0412:
SAEMRSA15_14390 (bfmBC)
JH1:
SaurJH1_1610
JH9:
SaurJH9_1577
JKD6008:
SAA6008_01488 (lpdA)
JKD6159:
SAA6159_01454 (lpdA)
JSNZ:
JSNZ_001509 (lpdA)
LGA251:
SARLGA251_14250 (bfmBC)
M013:
M013TW_1534
MRSA252:
SAR1596 (bfmBC)
MSHR1132:
SAMSHR1132_13590
MSSA476:
SAS1457
Mu3:
SAHV_1506
Mu50:
SAV1518
MW2:
MW1471
RF122:
SAB1391c
ST398:
SAPIG1584 (lpdA)
T0131:
SAT0131_01612
TCH60:
HMPREF0772_11622 (lpd)
TW20:
SATW20_15150 (bfmBC)
USA300_TCH1516:
USA300HOU_1520 (lpdA)
VC40:
SAVC_06840
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MSEKQYDLVVLGGGTAGYVAAIRASQLGKKVAIVERQLLGGTCLHKGCIPTKSLLKSAEV
N315 MSEKQYDLVVLGGGTAGYVAAIRASQLGKKVAIVERQLLGGTCLHKGCIPTKSLLKSAEV
NCTC8325 MSEKQYDLVVLGGGTAGYVAAIRASQLGKKVAIVERQLLGGTCLHKGCIPTKSLLKSAEV
Newman MSEKQYDLVVLGGGTAGYVAAIRASQLGKKVAIVERQLLGGTCLHKGCIPTKSLLKSAEV
USA300_FPR3757 MSEKQYDLVVLGGGTAGYVAAIRASQLGKKVAIVERQLLGGTCLHKGCIPTKSLLKSAEV
************************************************************
COL FQTVKQAAMFGVDVKDANVNFENMLARKEDIINQMYQGVKHLMQHNHIDIYNGTGRILGT
N315 FQTVKQAAMFGVDVKDANVNFENMLARKEDIINQMYQGVKHLMQHNHIDIYNGTGRILGT
NCTC8325 FQTVKQAAMFGVDVKDANVNFENMLARKEDIINQMYQGVKHLMQHNHIDIYNGTGRILGT
Newman FQTVKQAAMFGVDVKDANVNFENMLARKEDIINQMYQGVKHLMQHNHIDIYNGTGRILGT
USA300_FPR3757 FQTVKQAAMFGVDVKDANVNFENMLARKEDIINQMYQGVKHLMQHNHIDIYNGTGRILGT
************************************************************
COL SIFSPQSGTISVEYEDGESDLLPNQFVLIATGSSPAELPFLSFDHDKILSSDDILSLKTL
N315 SIFSPQSGTISVEYEDGESDLLPNQFVLIATGSSPAELPFLSFDHDKILSSDDILSLKTL
NCTC8325 SIFSPQSGTISVEYEDGESDLLPNQFVLIATGSSPAELPFLSFDHDKILSSDDILSLKTL
Newman SIFSPQSGTISVEYEDGESDLLPNQFVLIATGSSPAELPFLSFDHDKILSSDDILSLKTL
USA300_FPR3757 SIFSPQSGTISVEYEDGESDLLPNQFVLIATGSSPAELPFLSFDHDKILSSDDILSLKTL
************************************************************
COL PSSIGIIGGGVIGMEFASLMIDLGVDVTVIEAGERILPTESKQASQLLKKSLSARGVKFY
N315 PSSIGIIGGGVIGMEFASLMIDLGVDVTVIEAGERILPTESKQASQLLKKSLSARGVKFY
NCTC8325 PSSIGIIGGGVIGMEFASLMIDLGVDVTVIEAGERILPTESKQASQLLKKSLSARGVKFY
Newman PSSIGIIGGGVIGMEFASLMIDLGVDVTVIEAGERILPTESKQASQLLKKSLSARGVKFY
USA300_FPR3757 PSSIGIIGGGVIGMEFASLMIDLGVDVTVIEAGERILPTESKQASQLLKKSLSARGVKFY
************************************************************
COL EGIKLSENDINVNEDGVTFEISSDIIKVDKVLLSIGRKPNTSDIGLNNTKIKLSTSGHIL
N315 EGIKLSENDINVNEDGVTFEISSDIIKVDKVLLSIGRKPNTSDIGLNNTKIKLSTSGHIL
NCTC8325 EGIKLSENDINVNEDGVTFEISSDIIKVDKVLLSIGRKPNTSDIGLNNTKIKLSTSGHIL
Newman EGIKLSENDINVNEDGVTFEISSDIIKVDKVLLSIGRKPNTSDIGLNNTKIKLSTSGHIL
USA300_FPR3757 EGIKLSENDINVNEDGVTFEISSDIIKVDKVLLSIGRKPNTSDIGLNNTKIKLSTSGHIL
************************************************************
COL TNEFQQTEDKHIYAAGDCIGKLQLAHVGSKEGVVAVDHMFEGNPIPVNYNMMPKCIYSQP
N315 TNEFQQTEDKHIYAAGDCIGKLQLAHVGSKEGVVAVDHMFEGNPIPVNYNMMPKCIYSQP
NCTC8325 TNEFQQTEDKHIYAAGDCIGKLQLAHVGSKEGVVAVDHMFEGNPIPVNYNMMPKCIYSQP
Newman TNEFQQTEDKHIYAAGDCIGKLQLAHVGSKEGVVAVDHMFEGNPIPVNYNMMPKCIYSQP
USA300_FPR3757 TNEFQQTEDKHIYAAGDCIGKLQLAHVGSKEGVVAVDHMFEGNPIPVNYNMMPKCIYSQP
************************************************************
COL EIASIGLNIEQAKAEGMKVKSFKVPFKAIGKAVIDSHDTNEGYSEMVIDQSTEEIVGINM
N315 EIASIGLNIEQAKAEGMKVKSFKVPFKAIGKAVIDSHDANEGYSEMVIDQSTEEIVGINM
NCTC8325 EIASIGLNIEQAKAEGMKVKSFKVPFKAIGKAVIDSHDTNEGYSEMVIDQSTEEIVGINM
Newman EIASIGLNIEQAKAEGMKVKSFKVPFKAIGKAVIDSHDTNEGYSEMVIDQSTEEIVGINM
USA300_FPR3757 EIASIGLNIEQAKAEGMKVKSFKVPFKAIGKAVIDSHDTNEGYSEMVIDQSTEEIVGINM
**************************************:*********************
COL IGPHVTELINEASLLQFMNGSALELGLTTHAHPSISEVLMELGLKAESRAIHV
N315 IGPHVTELINEASLLQFMNGSALELGLTTHAHPSISEVLMELGLKAESRAIHV
NCTC8325 IGPHVTELINEASLLQFMNGSALELGLTTHAHPSISEVLMELGLKAESRAIHV
Newman IGPHVTELINEASLLQFMNGSALELGLTTHAHPSISEVLMELGLKAESRAIHV
USA300_FPR3757 IGPHVTELINEASLLQFMNGSALELGLTTHAHPSISEVLMELGLKAESRAIHV
*****************************************************
- ↑ Vineet K Singh, Dipti S Hattangady, Efstathios S Giotis, Atul K Singh, Neal R Chamberlain, Melissa K Stuart, Brian J Wilkinson
Insertional inactivation of branched-chain alpha-keto acid dehydrogenase in Staphylococcus aureus leads to decreased branched-chain membrane fatty acid content and increased susceptibility to certain stresses.
Appl Environ Microbiol: 2008, 74(19);5882-90
[PubMed:18689519] [WorldCat.org] [DOI] (I p)Vineet K Singh, Sirisha Sirobhushanam, Robert P Ring, Saumya Singh, Craig Gatto, Brian J Wilkinson
Roles of pyruvate dehydrogenase and branched-chain α-keto acid dehydrogenase in branched-chain membrane fatty acid levels and associated functions in Staphylococcus aureus.
J Med Microbiol: 2018, 67(4);570-578
[PubMed:29498620] [WorldCat.org] [DOI] (I p)