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⊟Summary[edit | edit source]
- pan ID?: SAUPAN003858000
- symbol?: dfrA
- synonym:
- description?: dihydrofolate reductase
- dihydrofolate reductase
- dihydrofolate reductase type 1 from Tn4003
- dihydrofolate reductase type I
- trimethoprim-sensitive dihydrofolate reductase
descriptions from strain specific annotations:
- strand?: -
- coordinates?: 4139681..4140160
- synteny block?: BlockID0029140
- occurrence?: in 100% of 34 strains
dfrA (folA) : dihydrofolate reductase [1]
• A crystal structure is available : 3FRF
Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Target of the antibiotic trimethoprim.
⊟Orthologs[edit | edit source]
04-02981:
SA2981_1380 (dfrA)
08BA02176:
C248_1466 (dfrB)
11819-97:
MS7_1382 (folA)
6850:
RSAU_001305 (dfrA)
71193:
ST398NM01_1427
ECT-R 2:
ECTR2_1282
ED133:
SAOV_1435c
ED98:
SAAV_1409 (folA)
HO 5096 0412:
SAEMRSA15_12890 (dfrB)
JH1:
SaurJH1_1515
JH9:
SaurJH9_1486
JKD6008:
SAA6008_01394 (dfrA)
JKD6159:
SAA6159_01293 (dfrA)
JSNZ:
JSNZ_001411
LGA251:
SARLGA251_13420 (dfrB)
M013:
M013TW_1372
MRSA252:
SAR1439 (dfrB)
MSHR1132:
SAMSHR1132_12680
MSSA476:
SAS1369
Mu3:
SAHV_1414 (dfrA)
Mu50:
SAV1426 (dfrA)
MW2:
MW1316 (dfrA)
RF122:
SAB1281c (dfrB)
ST398:
SAPIG1427 (folA)
T0131:
SAT0131_01506 (guaB)
TCH60:
HMPREF0772_11780 (folA)
TW20:
SATW20_14270 (dfrB)
USA300_TCH1516:
USA300HOU_1363 (dfrB)
VC40:
SAVC_06380
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMGRKTFESIGKPLPNRRN
N315 MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMGRKTFESIGKPLPNRRN
NCTC8325 MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMGRKTFESIGKPLPNRRN
Newman MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMGRKTFESIGKPLPNRRN
USA300_FPR3757 MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMGRKTFESIGKPLPNRRN
************************************************************
COL VVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEMIDKVDDMYITVIEGKFRG
N315 VVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEMIDKVDDMYITVIEGKFRG
NCTC8325 VVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEMIDKVDDMYITVIEGKFRG
Newman VVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEMIDKVDDMYITVIEGKFRG
USA300_FPR3757 VVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEMIDKVDDMYITVIEGKFRG
************************************************************
COL DTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK
N315 DTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK
NCTC8325 DTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK
Newman DTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK
USA300_FPR3757 DTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK
***************************************
- ↑ M Frosolono, S L Hodel-Christian, B E Murray
Lack of homology of enterococci which have high-level resistance to trimethoprim with the dfrA gene of Staphylococcus aureus.
Antimicrob Agents Chemother: 1991, 35(9);1928-30
[PubMed:1952870] [WorldCat.org] [DOI] (P p)D A Rouch, L J Messerotti, L S Loo, C A Jackson, R A Skurray
Trimethoprim resistance transposon Tn4003 from Staphylococcus aureus encodes genes for a dihydrofolate reductase and thymidylate synthetase flanked by three copies of IS257.
Mol Microbiol: 1989, 3(2);161-75
[PubMed:2548057] [WorldCat.org] [DOI] (P p)G E Dale, C Broger, A D'Arcy, P G Hartman, R DeHoogt, S Jolidon, I Kompis, A M Labhardt, H Langen, H Locher, M G Page, D Stüber, R L Then, B Wipf, C Oefner
A single amino acid substitution in Staphylococcus aureus dihydrofolate reductase determines trimethoprim resistance.
J Mol Biol: 1997, 266(1);23-30
[PubMed:9054967] [WorldCat.org] [DOI] (P p)Christian Oefner, Monica Bandera, Andreas Haldimann, Heike Laue, Henk Schulz, Seema Mukhija, Sandro Parisi, Laurent Weiss, Sergio Lociuro, Glenn E Dale
Increased hydrophobic interactions of iclaprim with Staphylococcus aureus dihydrofolate reductase are responsible for the increase in affinity and antibacterial activity.
J Antimicrob Chemother: 2009, 63(4);687-98
[PubMed:19211577] [WorldCat.org] [DOI] (I p)