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⊟Summary[edit | edit source]
- pan ID?: SAUPAN003536000
- symbol?: rnhB
- synonym:
- description?: ribonuclease HII
- ribonuclease HII
- putative ribonuclease HII
- ribonuclease HII (RNase HII)
- ribonuclease HII family protein
descriptions from strain specific annotations:
- strand?: +
- coordinates?: 3875507..3876274
- synteny block?: BlockID0026130
- occurrence?: in 100% of 34 strains
rnhB : ribonuclease H-II [1]
• A crystal structure is available : 5Y9P
Staphylococci produce different paralogues of Ribonuclease H to introduce nicks in different RNA-DNA hybrid structures. Occasional misincorporation of single ribonucleotides in DNA duplex polymers can be managed by ribonucleotide excision repair. RNase H-II introduces a nick 5'- to a single ribonucleotide in a mostly DNA-DNA duplex. The nick allows DNA Pol I access to the ribonucleotide which it removes with its 5'→3' exonuclease activity and then fills in with a deoxyribonucleotide.
⊟Orthologs[edit | edit source]
04-02981:
SA2981_1202 (rnhB)
08BA02176:
C248_1275 (rnhB)
11819-97:
MS7_1202
6850:
RSAU_001126 (rnhB)
71193:
ST398NM01_1244
ECT-R 2:
ECTR2_1100
ED133:
SAOV_1247
ED98:
SAAV_1218 (rnhB)
HO 5096 0412:
SAEMRSA15_10770 (rnhB)
JH1:
SaurJH1_1328 (rnhB)
JH9:
SaurJH9_1303 (rnhB)
JKD6008:
SAA6008_01199 (rnhB)
JKD6159:
SAA6159_01099 (rnhB)
JSNZ:
JSNZ_001214
LGA251:
SARLGA251_11550 (rnhB)
M013:
M013TW_1182
MRSA252:
SAR1220 (rnhB)
MSHR1132:
SAMSHR1132_10870
MSSA476:
SAS1178 (rnhB)
Mu3:
SAHV_1234 (rnhB)
Mu50:
SAV1244 (rnhB)
MW2:
MW1127 (rnhB)
RF122:
SAB1108 (rnhB)
ST398:
SAPIG1244
T0131:
SAT0131_01294
TCH60:
HMPREF0772_11987 (rnhB)
TW20:
SATW20_12380 (rnhB)
USA300_TCH1516:
USA300HOU_1175 (rnhB)
VC40:
SAVC_05385 (rnhB)
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MTLTIKEVTQLINAVNTIEELENHECFLDERKGVQNAIARRRKALEKEQALKEKYVEMTY
N315 MTLTIKEVTQLINAVNTIEELENHECFLDERKGVQNAIARRRKALEKEQALKEKYVEMTY
NCTC8325 MTLTIKEVTQLINAVNTIEELENHECFLDERKGVQNAIARRRKALEKEQALKEKYVEMTY
Newman MTLTIKEVTQLINAVNTIEELENHECFLDERKGVQNAIARRRKALEKEQALKEKYVEMTY
USA300_FPR3757 MTLTIKEVTQLINAVNTIEELENHECFLDERKGVQNAIARRRKALEKEQALKEKYVEMTY
************************************************************
COL FENEILKEHPNAIICGIDEVGRGPLAGPVVACATILNSNHNYLGLDDSKKVPVTKRLELN
N315 FENEILKEHPNAIICGIDEVGRGPLAGPVVACATILNSNHNYLGLDDSKKVPITKRLELN
NCTC8325 FENEILKEHPNAIICGIDEVGRGPLAGPVVACATILNSNHNYLGLDDSKKVPVTKRLELN
Newman FENEILKEHPNAIICGIDEVGRGPLAGPVVACATILNSNHNYLGLDDSKKVPVTKRLELN
USA300_FPR3757 FENEILKEHPNAIICGIDEVGRGPLAGPVVACATILNSNHNYLGLDDSKKVPVTKRLELN
****************************************************:*******
COL EALKNEVTAFAYGIATAEEIDEFNIYKATQIAMQRAIDGLSVQPTHLLIDAMTLDNALPQ
N315 EALKNEVTAFAYGIATAEEIDEFNIYKATQIAMQRAIDGLSVQPTHLLIDAMTLDNALPQ
NCTC8325 EALKNEVTAFAYGIATAEEIDEFNIYKATQIAMQRAIDGLSVQPTHLLIDAMTLDNALPQ
Newman EALKNEVTAFAYGIATAEEIDEFNIYKATQIAMQRAIDGLSVQPTHLLIDAMTLDNALPQ
USA300_FPR3757 EALKNEVTAFAYGIATAEEIDEFNIYKATQIAMQRAIDGLSVQPTHLLIDAMTLDNALPQ
************************************************************
COL VSLIKGDARSVSIAAASIMAKVFRDDYMTQLSKDYPEYGFEKNAGYGTKQHLLAIDDIGI
N315 VSLIKGDARSVSIAAASIMAKVFRDDYMTQLSKDYPEYGFEKNAGYGTKQHLLAIDDIGI
NCTC8325 VSLIKGDARSVSIAAASIMAKVFRDDYMTQLSKDYPEYGFEKNAGYGTKQHLLAIDDIGI
Newman VSLIKGDARSVSIAAASIMAKVFRDDYMTQLSKDYPEYGFEKNAGYGTKQHLLAIDDIGI
USA300_FPR3757 VSLIKGDARSVSIAAASIMAKVFRDDYMTQLSKDYPEYGFEKNAGYGTKQHLLAIDDIGI
************************************************************
COL MKEHRKSFEPIKSLL
N315 MKEHRKSFEPIKSLL
NCTC8325 MKEHRKSFEPIKSLL
Newman MKEHRKSFEPIKSLL
USA300_FPR3757 MKEHRKSFEPIKSLL
***************
- ↑ Scott A Reiling, Kohei Homma, Oluwatoyin A Asojo
Purification and crystallization of RNase HIII from Staphylococcus aureus.
Acta Crystallogr Sect F Struct Biol Cryst Commun: 2011, 67(Pt 1);79-82
[PubMed:21206030] [WorldCat.org] [DOI] (I p)Justin R Randall, William G Hirst, Lyle A Simmons
Substrate Specificity for Bacterial RNases HII and HIII Is Influenced by Metal Availability.
J Bacteriol: 2018, 200(4);
[PubMed:29084857] [WorldCat.org] [DOI] (I e)Justin R Randall, Taylor M Nye, Katherine J Wozniak, Lyle A Simmons
RNase HIII Is Important for Okazaki Fragment Processing in Bacillus subtilis.
J Bacteriol: 2019, 201(7);
[PubMed:30670546] [WorldCat.org] [DOI] (I e)Anna Lipońska, Mee-Ngan F Yap
Hibernation-Promoting Factor Sequesters Staphylococcus aureus Ribosomes to Antagonize RNase R-Mediated Nucleolytic Degradation.
mBio: 2021, 12(4);e0033421
[PubMed:34253058] [WorldCat.org] [DOI] (I p)