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⊟Summary[edit | edit source]
- pan ID?: SAUPAN003501000
- symbol?: rlmN
- synonym:
- description?: 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN
- 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN
- ribosomal RNA large subunit methyltransferase N
- ribosomal RNA large subunit methyltransferase N, putative
- 23S rRNA methyltransferase
- cfr family radical SAM enzyme
- radical SAM enzyme, Cfr family
- radical SAM protein
- radical SAM superfamily protein
- Ribosomal RNA large subunit methyltransferase N, Rlmn
descriptions from strain specific annotations:
- strand?: +
- coordinates?: 3840798..3841892
- synteny block?: BlockID0025800
- occurrence?: in 100% of 34 strains
rlmN : ribosome large subunit and tRNA methyltransferase N [1]
Ribosome large subunit and tRNA methyltransferase N (alt: 23S rRNA adenosine (2530)- and tRNA adenosine(37)-C(2)-methyltransferase) attaches a methyl group to the adenosine nucleobase 2-position of residue 2530 in 23S ribosomal RNA (S. aureus numbering; adenosine 2503 by E. coli numbering). RlmN is a dual-function enzyme and is also able to attach a methyl group to adenosine nucleobases at the anticodon loop-adjacent residue 37 of transfer RNAs. This modification enhances proofreading at the peptidyl transferase center (23S rRNA) and a more relaxed tRNA confirmation allowing for greater processivity.
- 23S rRNA : A (2530) → m2A (2530)
- tRNA : A (37) → m2A (37)
⊟Orthologs[edit | edit source]
04-02981:
SA2981_1176
08BA02176:
C248_1248
11819-97:
MS7_1176 (rlmN)
6850:
RSAU_001100 (rlmN)
71193:
ST398NM01_1217
ECT-R 2:
ECTR2_1074
ED133:
SAOV_1221
ED98:
SAAV_1190
HO 5096 0412:
SAEMRSA15_10510
JH1:
SaurJH1_1302
JH9:
SaurJH9_1277
JKD6008:
SAA6008_01173 (rlmN)
JKD6159:
SAA6159_01071 (rlmN)
JSNZ:
JSNZ_001187 (rlmN)
LGA251:
SARLGA251_11290 (rlmN)
M013:
M013TW_1156
MRSA252:
SAR1194
MSHR1132:
SAMSHR1132_10590
MSSA476:
SAS1152
Mu3:
SAHV_1208
Mu50:
SAV1218
MW2:
MW1101
RF122:
SAB1082
ST398:
SAPIG1217
T0131:
SAT0131_01267 (modA)
TCH60:
HMPREF0772_12014
TW20:
SATW20_12120 (rlmN)
USA300_TCH1516:
USA300HOU_1155
VC40:
SAVC_05255
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAKQIFEWLYQKRVDSIDE
N315 MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAKQIFEWLYQKRVDSIDE
NCTC8325 MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAKQIFEWLYQKRVDSIDE
Newman MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAKQIFEWLYQKRVDSIDE
USA300_FPR3757 MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAKQIFEWLYQKRVDSIDE
************************************************************
COL MTNLSKDLRQLLKDNFTVTTLTTVVKQESKDGTIKFLFELQDGYTIETVLMRHDYGNSVC
N315 MTNLSKDLRQLLKDNFTVTTLTTVVKQESKDGTIKFLFELQDGYTIETVLMRHDYGNSVC
NCTC8325 MTNLSKDLRQLLKDNFTVTTLTTVVKQESKDGTIKFLFELQDGYTIETVLMRHDYGNSVC
Newman MTNLSKDLRQLLKDNFTVTTLTTVVKQESKDGTIKFLFELQDGYTIETVLMRHDYGNSVC
USA300_FPR3757 MTNLSKDLRQLLKDNFTVTTLTTVVKQESKDGTIKFLFELQDGYTIETVLMRHDYGNSVC
************************************************************
COL VTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKALDATEERVSQIVIMGIGEPF
N315 VTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKALDATEERVSQIVIMGIGEPF
NCTC8325 VTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKALDATEERVSQIVIMGIGEPF
Newman VTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKALDATEERVSQIVIMGIGEPF
USA300_FPR3757 VTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKALDATEERVSQIVIMGIGEPF
************************************************************
COL ENYDEMMDFLRIVNDDNSLNIGARHITVSTSGIIPRIYDFADEDIQINFAVSLHAAKDEV
N315 ENYDEMMDFLRIVNDDNSLNIGARHITVSTSGIIPRIYDFADEDIQINFAVSLHAAKDEV
NCTC8325 ENYDEMMDFLRIVNDDNSLNIGARHITVSTSGIIPRIYDFADEDIQINFAVSLHAAKDEV
Newman ENYDEMMDFLRIVNDDNSLNIGARHITVSTSGIIPRIYDFADEDIQINFAVSLHAAKDEV
USA300_FPR3757 ENYDEMMDFLRIVNDDNSLNIGARHITVSTSGIIPRIYDFADEDIQINFAVSLHAAKDEV
************************************************************
COL RSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYGLFGGVNDQLEHARELAHLIKGLNC
N315 RSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYGLFGGVNDQLEHARELAHLIKGLNC
NCTC8325 RSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYGLFGGVNDQLEHARELAHLIKGLNC
Newman RSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYGLFGGVNDQLEHARELAHLIKGLNC
USA300_FPR3757 RSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYGLFGGVNDQLEHARELAHLIKGLNC
************************************************************
COL HVNLIPVNHVPERNYVKTAKNDIFKFEKELKRLGINATIRREQGSDIDAACGQLRAKERQ
N315 HVNLIPVNHVPERNYVKTAKNDIFKFEKELKRLGINATIRREQGSDIDAACGQLRAKERQ
NCTC8325 HVNLIPVNHVPERNYVKTAKNDIFKFEKELKRLGINATIRREQGSDIDAACGQLRAKERQ
Newman HVNLIPVNHVPERNYVKTAKNDIFKFEKELKRLGINATIRREQGSDIDAACGQLRAKERQ
USA300_FPR3757 HVNLIPVNHVPERNYVKTAKNDIFKFEKELKRLGINATIRREQGSDIDAACGQLRAKERQ
************************************************************
COL VETR
N315 VETR
NCTC8325 VETR
Newman VETR
USA300_FPR3757 VETR
****
- ↑ Alfonso Benítez-Páez, Magda Villarroya, M-Eugenia Armengod
The Escherichia coli RlmN methyltransferase is a dual-specificity enzyme that modifies both rRNA and tRNA and controls translational accuracy.
RNA: 2012, 18(10);1783-95
[PubMed:22891362] [WorldCat.org] [DOI] (I p)Alexander Golubev, Bulat Fatkhullin, Iskander Khusainov, Lasse Jenner, Azat Gabdulkhakov, Shamil Validov, Gulnara Yusupova, Marat Yusupov, Konstantin Usachev
Cryo-EM structure of the ribosome functional complex of the human pathogen Staphylococcus aureus at 3.2 Å resolution.
FEBS Lett: 2020, 594(21);3551-3567
[PubMed:32852796] [WorldCat.org] [DOI] (I p)Adrián González-López, Daniel S D Larsson, Ravi Kiran Koripella, Brett N Cain, Martin Garcia Chavez, Paul J Hergenrother, Suparna Sanyal, Maria Selmer
Structures of the Staphylococcus aureus ribosome inhibited by fusidic acid and fusidic acid cyclopentane.
Sci Rep: 2024, 14(1);14253
[PubMed:38902339] [WorldCat.org] [DOI] (I e)