⊟Summary[edit | edit source]
- pan ID?: SAUPAN003402000
- symbol?: efb
- synonym:
- description?: fibrinogen-binding protein
- fibrinogen-binding protein
- complement convertase inhibitor Efb
- extracellular fibrinogen/complement-binding protein
- extracellular fibrinogen-binding protein
- Extracellular fibrinogen-binding protein Efb
- fibrinogen-binding protein precursor
- pseudogene
descriptions from strain specific annotations:
- strand?: +
- coordinates?: 3757926..3760626
- synteny block?: BlockID0024850
- occurrence?: in 100% of 34 strains
efb : staphylococcal fibrinogen- and complement C3b-binding immunomodulatory protein Efb [1]
• A crystal structure is available : 3D5R
The second staphylococcal core genome immune evasion cluster (IEC-2) encodes the dual-function extracellular fibrinogen- and complement C3b-binding immunomodulatory protein Efb. This protein prevents complement C5 activation and adaptive immunity through its C-terminal domain by forming higher order oligomers with cell surface-associated C3b and either itself or Efb. Through its N-terminal domain, it also binds to fibrinogen and platelets, cross-linking C3d-opsonized bacteria to a platelet-fibrinogen "shield" that prevents phagocytosis and can form a biofilm-like matrix. Because it only binds to C3b/d, Efb only prevents C3 deposition onto the staphylococcal cell by the alternative activation pathway, allowing opsonization and C3d processing by the classical or lectin pathways. However, membrane-bound C3d complement maturation into activated C5 convertase is blocked by Efb (or Ecb binding). C3b/C3d binding also prevents host B-cell and T-cell adaptive immunity via blocking of immune cell CR2 receptor/staphylococcal-opsonized-C3d coactivation. Finally, Efb released into a host immune cell's cytoplasm by intracellular staphylococci alters the immune activation cascade, blunting the inflammatory immune responses. Efb contains a signal peptidase secretion signal and is cleaved between alanine-29 and serine-30 to generate mature Efb.
⊟Orthologs[edit | edit source]
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MKNKLIAKSLLTLAAIGITTTTIASTADASEGYGPREKKPVSINHNIVEYNDGTFKYQSR
N315 MKNKLIAKSLLTIAAIGITTTTIASTADASEGYGPREKKPVSINHNIVEYNDGTFKYQSR
NCTC8325 MKNKLIAKSLLTLAAIGITTTTIASTADASEGYGPREKKPVSINHNIVEYNDGTFKYQSR
Newman MKNKLIAKSLLTLAAIGITTTTIASTADASEGYGPREKKPVSINHNIVEYNDGTFKYQSR
USA300_FPR3757 MKNKLIAKSLLTLAAIGITTTTIASTADASEGYGPREKKPVSINHNIVEYNDGTFKYQSR
************:***********************************************
COL PKFNSTPKYIKFKHDYNILEFNDGTFEYGARPQFNKPAAKTDATIKKEQKLIQAQNLVRE
N315 PKFNSTPKYIKFKHDYNILEFNDGTFEYGARPQFNKPAAKTDATIKKEQKLIQAQNLVRE
NCTC8325 PKFNSTPKYIKFKHDYNILEFNDGTFEYGARPQFNKPAAKTDATIKKEQKLIQAQNLVRE
Newman PKFNSTPKYIKFKHDYNILEFNDGTFEYGARPQFNKPAAKTDATIKKEQKLIQAQNLVRE
USA300_FPR3757 PKFNSTPKYIKFKHDYNILEFNDGTFEYGARPQFNKPAAKTDATIKKEQKLIQAQNLVRE
************************************************************
COL FEKTHTVSAHRKAQKAVNLVSFEYKVKKMVLQERIDNVLKQGLVK
N315 FEKTHTVSAHRKAQKAVNLVSFEYKVKKMVLQERIDNVLKQGLVR
NCTC8325 FEKTHTVSAHRKAQKAVNLVSFEYKVKKMVLQERIDNVLKQGLVK
Newman FEKTHTVSAHRKAQKAVNLVSFEYKVKKMVLQERIDNVLKQGLVK
USA300_FPR3757 FEKTHTVSAHRKAQKAVNLVSFEYKVKKMVLQERIDNVLKQGLVK
********************************************:
- ↑ Ilse Jongerius, Jörg Köhl, Manoj K Pandey, Maartje Ruyken, Kok P M van Kessel, Jos A G van Strijp, Suzan H M Rooijakkers
Staphylococcal complement evasion by various convertase-blocking molecules.
J Exp Med: 2007, 204(10);2461-71
[PubMed:17893203] [WorldCat.org] [DOI] (P p)Brandon L Garcia, Kasra X Ramyar, Daniel Ricklin, John D Lambris, Brian V Geisbrecht
Advances in understanding the structure, function, and mechanism of the SCIN and Efb families of Staphylococcal immune evasion proteins.
Adv Exp Med Biol: 2012, 946;113-33
[PubMed:21948365] [WorldCat.org] [DOI] (P p)Xiaokai Zhang, Tingrong Xiong, Lin Gao, Yu Wang, Luxuan Liu, Tian Tian, Yun Shi, Jinyong Zhang, Zhuo Zhao, Dongshui Lu, Ping Luo, Weijun Zhang, Ping Cheng, Haiming Jing, Qiang Gou, Hao Zeng, Dapeng Yan, Quanming Zou
Extracellular fibrinogen-binding protein released by intracellular Staphylococcus aureus suppresses host immunity by targeting TRAF3.
Nat Commun: 2022, 13(1);5493
[PubMed:36123338] [WorldCat.org] [DOI] (I e)