⊟Summary[edit | edit source]
- pan ID?: SAUPAN003348000
- symbol?: —
- synonym:
- description?: glycerophosphodiester phosphodiesterase
- glycerophosphodiester phosphodiesterase
- glycerophosphoryl diester phosphodiesterase
- glycerophosphoryl diester phosphodiesterase, putative
- glycerophosphoryl diester phosphodiesterase family protein
- exported protein
descriptions from strain specific annotations:
- strand?: -
- coordinates?: 3715631..3716741
- synteny block?: BlockID0024320
- occurrence?: in 97% of 34 strains
lpgD : lysophosphatidylglycerol phospholipase D [1]
• A crystal structure is available : 8GHI
The staphylococcal phospholipid bilayer consists almost exclusively of phosphatidylglycerol and its derivatives. Staphylococcal phosphatidylglycerol consists of a glycerol molecule decorated with a C16 or longer fatty acid ester at the (1) hydroxyl, an anteiso-pentadecanoate ester at the (2) hydroxyl and a phosphoglycerate phosphoester at the (3) hydroxyl. During phospholipid recycling, the first step is to remove one of the fatty acids. Typically, the C16(+) fatty acid at position (1) is removed by Glycerol Ester Hydrolase resulting in anteiso-pentadecanoyl-lysophosphatidylglycerol (2-(a15)-LPG). However, occasionally the pentadecanoate fatty acid at position (2) is removed resulting in 1-(≥16)-LPG. This alternative 1-(≥16)-LPG degradation product requires further processing to a lysophosphatidic acid by Lysophosphatidylglycerol Phospholipase D removal of glycerol from the phosphoglycerate moiety at the (3) position before it can re-enter the phospholipid recycling pathway.
Note: the anteiso-pentadecanoate is more properly characterized as 12(S)methyltetradecanoate but due to the more common description of "pentadecanoate" in the literature, that is the term used here.
⊟Orthologs[edit | edit source]
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MKKVNRNILNAVLVGAGFLSSLLMINKNKVITKKQTIPAFFKGNAPYIFAHRGGMALRPE
N315 MKKVNRNILNAVLVGAGFLSSLLMINKNKVITKKQTIPAFFKGNAPYIFAHRGGMALRPE
NCTC8325 MKKVNRNILNAVLVGAGFLSSLLMINKNKVITKKQTIPAFFKGNAPYIFAHRGGMALRPE
Newman MKKVNRNILNAVLVGAGFLSSLLMINKNKVITKKQTIPAFFKGNAPYIFAHRGGMALRPE
USA300_FPR3757 MKKVNRNILNAVLVGAGFLSSLLMINKNKVITKKQTIPAFFKGNAPYIFAHRGGMALRPE
************************************************************
COL QTQLAFDYAKQLGVDGFETDVRLTKDQQLIVFHDATVDRTTNGSGKVSAHTLAELKKLDA
N315 QTQLAFDYAKQLGVDGFETDVRLTKDQQLIVFHDATVDRTTNGSGKVSAHTLAELKKLDA
NCTC8325 QTQLAFDYAKQLGVDGFETDVRLTKDQQLIVFHDATVDRTTNGSGKVSAHTLAELKKLDA
Newman QTQLAFDYAKQLGVDGFETDVRLTKDQQLIVFHDATVDRTTNGSGKVSAHTLAELKKLDA
USA300_FPR3757 QTQLAFDYAKQLGVDGFETDVRLTKDQQLIVFHDATVDRTTNGSGKVSAHTLAELKKLDA
************************************************************
COL AYHFKDINGLTPYRGHAHTAILTFDELLKQYPDMYINVDLKDAPESYEGSIAPQIMFDTI
N315 AYHFKDINGLTPYRGHAHTTILTFDELLKQYPDMYINVDLKDAPESYEGSIAPQIMFDTI
NCTC8325 AYHFKDINGLTPYRGHAHTAILTFDELLKQYPDMYINVDLKDAPESYEGSIAPQIMFDTI
Newman AYHFKDINGLTPYRGHAHTAILTFDELLKQYPDMYINVDLKDAPESYEGSIAPQIMFDTI
USA300_FPR3757 AYHFKDINGLTPYRGHAHTAILTFDELLKQYPDMYINVDLKDAPESYEGSIAPQIMFDTI
*******************:****************************************
COL AENQAFDRVLVTSFYKEQIVRFNKIAQGSVAIGASQQEVTEAFLKYHLLGGRYYQPLAQT
N315 AENQAFDRVLVTSFYKEQIVRFNKIAQGSVAIGASQQEVTEAFLKYHLLGGRYYQPLAQT
NCTC8325 AENQAFDRVLVTSFYKEQIVRFNKIAQGSVAIGASQQEVTEAFLKYHLLGGRYYQPLAQT
Newman AENQAFDRVLVTSFYKEQIVRFNKIAQGSVAIGASQQEVTEAFLKYHLLGGRYYQPLAQT
USA300_FPR3757 AENQAFDRVLVTSFYKEQIVRFNKIAQGSVAIGASQQEVTEAFLKYHLLGGRYYQPLAQT
************************************************************
COL FQMPTHFKGIDLTSSRFIKWLNDMNIIPGYYGVNSINLMNDLYQKGAHTIVTDRPDLAQQ
N315 FQMPTHFKGIDLTSSRFIKWLNDMNIIPGYYGVNSIDLMNDLYQKGAHTIVTDRPDLAQQ
NCTC8325 FQMPTHFKGIDLTSSRFIKWLNDMNIIPGYYGVNSINLMNDLYQKGAHTIVTDRPDLAQQ
Newman FQMPTHFKGIDLTSSRFIKWLNDMNIIPGYYGVNSINLMNDLYQKGAHTIVTDRPDLAQQ
USA300_FPR3757 FQMPTHFKGIDLTSSRFIKWLNDMNIIPGYYGVNSINLMNDLYQKGAHTIVTDRPDLAQQ
************************************:***********************
COL FKQTIPNK
N315 FKQTIPNK
NCTC8325 FKQTIPNK
Newman FKQTIPNK
USA300_FPR3757 FKQTIPNK
********
- ↑ Chitra Subramanian, Mi-Kyung Yun, Matthew M Frank, Charles O Rock
Lysophosphatidylglycerol (LPG) phospholipase D maintains membrane homeostasis in Staphylococcus aureus by converting LPG to lysophosphatidic acid.
J Biol Chem: 2023, 299(7);104863
[PubMed:37236358] [WorldCat.org] [DOI] (I p)Chitra Subramanian, Matthew W Frank, My-Kyung Yun, Charles O Rock
The Phospholipase A1 Activity of Glycerol Ester Hydrolase (Geh) Is Responsible for Extracellular 2-12(S)-Methyltetradecanoyl-Lysophosphatidylglycerol Production in Staphylococcus aureus.
mSphere: 2023, 8(2);e0003123
[PubMed:36976028] [WorldCat.org] [DOI] (I p)