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⊟Summary[edit | edit source]

  • pan ID?: SAUPAN003196000
  • symbol?: murE
  • synonym:
  • description?: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L- lysine ligase

      descriptions from strain specific annotations:

    • UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L- lysine ligase
    • UDP-N-acetylmuramoylalanyl-D-glutamate--L-lysine ligase
    • UDP-N-acetylmuramoylalanyl-D-glutamate--2,6- diaminopimelate ligase
    • UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate ligase
    • UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase (UDP-N-acetylmuramyl-tripeptide synthetase) (Meso-diaminopimelate-adding enzyme) (UDP-MurNAc-tripeptide synthetase)
  • strand?: +
  • coordinates?: 3588119..3589600
  • synteny block?: BlockID0022830
  • occurrence?: in 100% of 34 strains

murE : UDP-N-acetylmuramoyl-ʟ-alanyl-ᴅ-glutamate:ʟ-lysine ligase [1]

• A crystal structure is available : 4C12

MurE (alt: UDP-N-acetylmuramoyl-tripeptide synthase) catalyzes the addition of the third amino acid into the growing peptide stem of intracellular UDP-linked N-acetylmuramoyl-ʟ-alanyl-ᴅ-glutamate. Rather than condensing the α-amino group of lysine with the glutamate α-carboxylate in a traditional peptide bond linkage, MurE condenses the lysine α-amino group with the glutamyl-γ-carboxylate resulting in UDP-N-acetylmuramoyl-ʟ-alanyl-γ-ᴅ-glutamyl-ʟ-lysine.

⊟Orthologs[edit | edit source]

    COL:
    SACOL1023 (murE)
    N315:
    SA0876 (murE)
    NCTC8325:
    SAOUHSC_00954
    Newman:
    NWMN_0888 (murE)
    USA300_FPR3757:
    SAUSA300_0919 (murE)
    04-02981:
    SA2981_0972 (murE)
    08BA02176:
    C248_1042 (murE)
    11819-97:
    MS7_0974 (murE)
    6850:
    RSAU_000903 (murE)
    71193:
    ST398NM01_1014
    ECT-R 2:
    ECTR2_873 (murE)
    ED133:
    SAOV_0963
    ED98:
    SAAV_0980
    HO 5096 0412:
    SAEMRSA15_08480 (murE)
    JH1:
    SaurJH1_1035
    JH9:
    SaurJH9_1016
    JKD6008:
    SAA6008_00973 (murE)
    JKD6159:
    SAA6159_00874 (murE)
    JSNZ:
    JSNZ_000977
    LGA251:
    SARLGA251_09330 (murE)
    M013:
    M013TW_0943
    MRSA252:
    SAR0988 (murE)
    MSHR1132:
    SAMSHR1132_08650
    MSSA476:
    SAS0887
    Mu3:
    SAHV_1012 (murE)
    Mu50:
    SAV1018 (murE)
    MW2:
    MW0899 (murE)
    RF122:
    SAB0884 (murE)
    ST398:
    SAPIG1014
    T0131:
    SAT0131_01053 (deoC)
    TCH60:
    HMPREF0772_12216 (murE2)
    TW20:
    SATW20_10150 (murE)
    USA300_TCH1516:
    USA300HOU_0976 (murE1)
    VC40:
    SAVC_04255

⊟Genome Viewer[edit | edit source]

COL
N315
NCTC8325
Newman
USA300_FPR3757

⊟Alignments[edit | edit source]

  • alignment of orthologues:
    CLUSTAL format alignment by MAFFT L-INS-i (v7.307)


    COL             MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVGYTVDSHKFCQNVADQG
    N315            MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVGYTVDSHKFCQNVADQG
    NCTC8325        MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVGYTVDSHKFCQNVADQG
    Newman          MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVGYTVDSHKFCQNVADQG
    USA300_FPR3757  MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVGYTVDSHKFCQNVADQG
                    ************************************************************

    COL             CKLVVVNKEQSLPANVTQVVVPDTLRVASILAHTLYDYPSHQLVTFGVTGTNGKTSIATM
    N315            CKLVVVNKEQSLPANVTQVVVPDTLRVASILAHTLYDYPSHQLVTFGVTGTNGKTSIATM
    NCTC8325        CKLVVVNKEQSLPANVTQVVVPDTLRVASILAHTLYDYPSHQLVTFGVTGTNGKTSIATM
    Newman          CKLVVVNKEQSLPANVTQVVVPDTLRVASILAHTLYDYPSHQLVTFGVTGTNGKTSIATM
    USA300_FPR3757  CKLVVVNKEQSLPANVTQVVVPDTLRVASILAHTLYDYPSHQLVTFGVTGTNGKTSIATM
                    ************************************************************

    COL             IHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLTKKIKEAVDAGAESMTLEVSS
    N315            IHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLTKKIKEAVDAGAESMTLEVSS
    NCTC8325        IHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLTKKIKEAVDAGAESMTLEVSS
    Newman          IHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLTKKIKEAVDAGAESMTLEVSS
    USA300_FPR3757  IHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLTKKIKEAVDAGAESMTLEVSS
                    ************************************************************

    COL             HGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHAKSLLFSQLGEDLSKEKYVVLNN
    N315            HGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHAKSLLFSQLGEDLSKEKYVVLNN
    NCTC8325        HGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHAKSLLFSQLGEDLSKEKYVVLNN
    Newman          HGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHAKSLLFSQLGEDLSKEKYVVLNN
    USA300_FPR3757  HGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHAKSLLFSQLGEDLSKEKYVVLNN
                    ************************************************************

    COL             DDSFSEYLRTVTPYEVFSYGIDEEAQFMAKNIQESLQGVSFDFVTPFGTYPVKSPYVGKF
    N315            DDSFSEYLRTVTPYEVFSYGIDEEAQFMAKNIQESLQGVSFDFVTPFGTYPVKSPYVGKF
    NCTC8325        DDSFSEYLRTVTPYEVFSYGIDEEAQFMAKNIQESLQGVSFDFVTPFGTYPVKSPYVGKF
    Newman          DDSFSEYLRTVTPYEVFSYGIDEEAQFMAKNIQESLQGVSFDFVTPFGTYPVKSPYVGKF
    USA300_FPR3757  DDSFSEYLRTVTPYEVFSYGIDEEAQFMAKNIQESLQGVSFDFVTPFGTYPVKSPYVGKF
                    ************************************************************

    COL             NISNIMAAMIAVWSKGTSLETIIKAVENLEPVEGRLEVLDPSLPIDLIIDYAHTADGMNK
    N315            NISNIMAAMIAVWSKGTSLETIIKAVENLEPVEGRLEVLDPSLPIDLIIDYAHTADGMNK
    NCTC8325        NISNIMAAMIAVWSKGTSLETIIKAVENLEPVEGRLEVLDPSLPIDLIIDYAHTADGMNK
    Newman          NISNIMAAMIAVWSKGTSLETIIKAVENLEPVEGRLEVLDPSLPIDLIIDYAHTADGMNK
    USA300_FPR3757  NISNIMAAMIAVWSKGTSLETIIKAVENLEPVEGRLEVLDPSLPIDLIIDYAHTADGMNK
                    ************************************************************

    COL             LIDAVQPFVKQKLIFLVGMAGERDLTKTPEMGRVACRADYVIFTPDNPANDDPKMLTAEL
    N315            LIDAVQPFVKQKLIFLVGMAGERDLTKTPEMGRVACRADYVIFTPDNPANDDPKMLTAEL
    NCTC8325        LIDAVQPFVKQKLIFLVGMAGERDLTKTPEMGRVACRADYVIFTPDNPANDDPKMLTAEL
    Newman          LIDAVQPFVKQKLIFLVGMAGERDLTKTPEMGRVACRADYVIFTPDNPANDDPKMLTAEL
    USA300_FPR3757  LIDAVQPFVKQKLIFLVGMAGERDLTKTPEMGRVACRADYVIFTPDNPANDDPKMLTAEL
                    ************************************************************

    COL             AKGATHQNYIEFDDRAEGIKHAIDIAEPGDTVVLASKGREPYQIMPGHIKVPHRDDLIGL
    N315            AKGATHQNYIEFDDRAEGIKHAIDIAEPGDTVVLASKGREPYQIMPGHIKVPHRDDLIGL
    NCTC8325        AKGATHQNYIEFDDRAEGIKHAIDIAEPGDTVVLASKGREPYQIMPGHIKVPHRDDLIGL
    Newman          AKGATHQNYIEFDDRAEGIKHAIDIAEPGDTVVLASKGREPYQIMPGHIKVPHRDDLIGL
    USA300_FPR3757  AKGATHQNYIEFDDRAEGIKHAIDIAEPGDTVVLASKGREPYQIMPGHIKVPHRDDLIGL
                    ************************************************************

    COL             EAAYKKFGGGPVD-
    N315            EAAYKKFGGGPVDQ
    NCTC8325        EAAYKKFGGGPVD-
    Newman          EAAYKKFGGGPVD-
    USA300_FPR3757  EAAYKKFGGGPVD-
                    ************* 

  1. ↑ A M Ludovice, S W Wu, H de Lencastre
    Molecular cloning and DNA sequencing of the Staphylococcus aureus UDP-N-acetylmuramyl tripeptide synthetase (murE) gene, essential for the optimal expression of methicillin resistance.
    Microb Drug Resist: 1998, 4(2);85-90
    [PubMed:9650993] [WorldCat.org] [DOI] (P p)    Delphine Patin, Audrey Boniface, Andreja Kovač, Mireille Hervé, Sébastien Dementin, Hélène Barreteau, Dominique Mengin-Lecreulx, Didier Blanot
    Purification and biochemical characterization of Mur ligases from Staphylococcus aureus.
    Biochimie: 2010, 92(12);1793-800
    [PubMed:20659527] [WorldCat.org] [DOI] (I p)    Karen M Ruane, Adrian J Lloyd, Vilmos Fülöp, Christopher G Dowson, Hélène Barreteau, Audrey Boniface, Sébastien Dementin, Didier Blanot, Dominique Mengin-Lecreulx, Stanislav Gobec, Andréa Dessen, David I Roper
    Specificity determinants for lysine incorporation in Staphylococcus aureus peptidoglycan as revealed by the structure of a MurE enzyme ternary complex.
    J Biol Chem: 2013, 288(46);33439-48
    [PubMed:24064214] [WorldCat.org] [DOI] (I p)
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