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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40
⊟Summary[edit | edit source]
- pan ID?: SAUPAN001438000
- symbol?: —
- synonym:
- description?: hypothetical protein
- strand?: -
- coordinates?: 1710396..1710953
- synteny block?: BlockID0009160
- occurrence?: in 9% of 34 strains
ssb-rec (gp2.5) : prophage ssDNA-binding protein [1]
Single-stranded DNA-binding proteins (SSBs) protect 3'-ssDNA overhangs from degradation and are essential components of single-stranded DNA recombinases. There is powerful evolutionary pressure for structural diversity as SSBs are frequent targets for CRISPR-mediated anti-phage defenses and several SSB paralogues exist. This version is a hybrid prophage SSB with single-strand DNA annealing/recombinase activity. Prophage SSBs are typically paired with a single-strand DNA exonuclease and a single-strand DNA recombinase.
⊟Orthologs[edit | edit source]
04-02981:
—
08BA02176:
—
11819-97:
—
6850:
—
71193:
—
ECT-R 2:
—
ED133:
—
ED98:
—
HO 5096 0412:
—
JH1:
—
JH9:
—
JKD6008:
—
JKD6159:
—
JSNZ:
—
LGA251:
—
M013:
—
MRSA252:
—
MSHR1132:
—
MSSA476:
—
Mu3:
—
Mu50:
—
MW2:
—
RF122:
—
ST398:
—
T0131:
—
TCH60:
—
TW20:
—
USA300_TCH1516:
USA300HOU_1474
VC40:
—
⊟Genome Viewer[edit | edit source]
| NCTC8325 | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
NCTC8325 MKAKVLNKTKVITGKVRASYAHIFEPHSMQEGQEAKYSISLIIPKSDTSTIKAIEQAIEA
USA300_FPR3757 MKAKVLNKTKVITGKVRASYAHIFEPHSMQEGQESKYSISLIIPKSDTSTIKAIEQAIEA
**********************************:*************************
NCTC8325 AKEEGKVSKFGGKVPANLKLPLRDGDTEREDDVNYQDAYFINASSKQAPGIIDQNKIRLT
USA300_FPR3757 AKEEGKVSKFGGKVPANLKLPLRDGDTEREDDVNYQDAYFINASSKQAPGIIDQNKIRLT
************************************************************
NCTC8325 DSGTIVSGDYIRASINLFPFNTNGNKGIAVGLNNIQLVEKGEPLGGASAAEDDFDELDTD
USA300_FPR3757 DSGTVVSGDYIRASINLFPFNTNGNKGIAVGLNNIQLVEKGEPLGGASAAEDDFDELDTD
****:*******************************************************
NCTC8325 DEDFL
USA300_FPR3757 DEDFL
*****
- ↑ Maan M Neamah, Ignacio Mir-Sanchis, María López-Sanz, Sonia Acosta, Ignacio Baquedano, Andreas F Haag, Alberto Marina, Silvia Ayora, José R Penadés
Sak and Sak4 recombinases are required for bacteriophage replication in Staphylococcus aureus.
Nucleic Acids Res: 2017, 45(11);6507-6519
[PubMed:28475766] [WorldCat.org] [DOI] (I p)Anne Lopes, Jihane Amarir-Bouhram, Guilhem Faure, Marie-Agnès Petit, Raphaël Guerois
Detection of novel recombinases in bacteriophage genomes unveils Rad52, Rad51 and Gp2.5 remote homologs.
Nucleic Acids Res: 2010, 38(12);3952-62
[PubMed:20194117] [WorldCat.org] [DOI] (I p)Alfredo J Hernandez, Charles C Richardson
Gp2.5, the multifunctional bacteriophage T7 single-stranded DNA binding protein.
Semin Cell Dev Biol: 2019, 86;92-101
[PubMed:29588157] [WorldCat.org] [DOI] (I p)T Hollis, J M Stattel, D S Walther, C C Richardson, T Ellenberger
Structure of the gene 2.5 protein, a single-stranded DNA binding protein encoded by bacteriophage T7.
Proc Natl Acad Sci U S A: 2001, 98(17);9557-62
[PubMed:11481454] [WorldCat.org] [DOI] (P p)