PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

⊟Summary

• pan ID^?: SAUPAN000992000
• symbol^?: capN
• synonym:
• description^?: UDP-glucose 4-epimerase

  descriptions from strain specific annotations:

  • UDP-glucose 4-epimerase
  • capsular polysaccharide biosynthesis protein Cap5N
  • capsular polysaccharide type 5/8 biosynthesis epimerase CapN
  • cap5N protein/UDP-glucose 4-epimerase
  • capsular polysaccharide biosynthesis protein CapN
  • capsular polysaccharide synthesis protein Cap5N
  • capsular polysaccharide synthesis UDP-glucose 4-epimerase CapN, putative
  • Capsular polysaccharide synthesis enzyme Cap8N
  • capsular polysaccharide synthesis enzyme Cap5N
  • capsular polysaccharide synthesis enzyme CapN
  • NAD-dependent epimerase/dehydratase
  • NAD dependent epimerase/dehydratase family protein
  • polysaccharide biosynthesis family protein
  • UDP-N-acetyl-D-quinovosamine 4-epimerase
• strand^?: +
• coordinates^?: 1260968..1261855
• synteny block^?: BlockID0005250
• occurrence^?: in 100% of 34 strains

capN : capsule polysaccharide precursor synthesis enzyme UDP-N-acetyl-4-hexulose 4-ketoreductase

Staphylococci can produce up to 15 different capsules based on variants of the core trisaccharide N-acetyl-D-mannosaminouronate (ManNAcA) - N-acetyl-L-fucosamine (L-FucNAc) - N-acetyl-D-fucosamine (D-FucNAc). CapN catalyzes the second of two steps in the biosynthesis of UDP-N-acetyl-D-fucosamine (D-FucNAc), reducing the C-4 ketone group of UDP-N-acetyl-dideoxy-D-xylo-hex-4-ulose.

Historically, different capsule types were assigned their own nomenclature (i.e. Cap5N and Cap8N for the two most prevalent capsule types, CP5 and CP8). The CapN protein is 97% identical between Cap5O and Cap8O, provides the same function, and is therefore harmonized as CapN.

⊟Orthologs

COL:
SACOL0149 (cap5N)
N315:
SA0157 (capN)
NCTC8325:
SAOUHSC_00127
Newman:
NWMN_0108 (capN)
USA300_FPR3757:
SAUSA300_0165 (cap5N)
04-02981:
SA2981_0163 (capN)
08BA02176:
C248_0151 (capN)
11819-97:
MS7_0156
6850:
RSAU_000116 (capN)
71193:
ST398NM01_0176
ECT-R 2:
ECTR2_119
ED133:
SAOV_0109
ED98:
SAAV_0130 (cap5N)
HO 5096 0412:
SAEMRSA15_01280 (capN)
JH1:
SaurJH1_0153
JH9:
SaurJH9_0148
JKD6008:
SAA6008_00140 (cap5N)
JKD6159:
SAA6159_00146 (cap5N)
JSNZ:
JSNZ_000105 (capN)
LGA251:
SARLGA251_01360 (capN)
M013:
M013TW_0152
MRSA252:
SAR0164 (capN)
MSHR1132:
SAMSHR1132_01360
MSSA476:
SAS0137
Mu3:
SAHV_0161 (capN)
Mu50:
SAV0162 (capN)
MW2:
MW0137 (cap8N)
RF122:
SAB0103 (capN)
ST398:
SAPIG0176
T0131:
SAT0131_00153 (sodA)
TCH60:
HMPREF0772_10336 (cap5N)
TW20:
SATW20_01730 (capN)
USA300_TCH1516:
USA300HOU_0176 (cap5N)
VC40:
SAVC_00580

⊟Genome Viewer

COL
N315
NCTC8325
Newman
USA300_FPR3757

⊟Alignments

• alignment of orthologues:
  CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
  
  
  COL             MRKNILITGVHGYIGNALKDKLIEQGHQVDQINVRNQLWKSTSFKDYDVLIHTAALVHNN
  N315            MRKNILITGVHGYIGNALKDKLIEQGHQVDQINVRNQLWKSTSFKDYDVLIHTAALVHNN
  NCTC8325        MRKNILITGVHGYIGNALKDKLIEQGHQVDQINVRNQLWKSTSFKDYDVLIHTAALVHNN
  Newman          MRKNILITGVHGYIGNALKDKLIEQGHQVDQINVRNQLWKSTSFKDYDVLIHTAALVHNN
  USA300_FPR3757  MRKNILITGVHGYIGNALKDKLIEQGHQVDQINVRNQLWKSTSFKDYDVLIHTAALVHNN
                  ************************************************************
  
  COL             SPQARLSDYMQVNMLLTKQLAQKAKAEDVKQFIFMSTMAVYGKEGHVGKSDQVDTQTPMN
  N315            SPQARLSDYMQVNMLLTKQLAQKAKAEDVKQFIFMSTMAVYGKEGQVGKSDQIDTQTPMN
  NCTC8325        SPQARLSDYMQVNMLLTKQLAQKAKAEDVKQFIFMSTMAVYGKEGHVGKSDQVDTQTPMN
  Newman          SPQARLSDYMQVNMLLTKQLAQKAKAEDVKQFIFMSTMAVYGKEGHVGKSDQVDTQTPMN
  USA300_FPR3757  SPQARLSDYMQVNMLLTKQLAQKAKAEDVKQFIFMSTMAVYGKEGHVGKSDQVDTQTPMN
                  *********************************************:******:*******
  
  COL             PTTNYGISKKFAEQALQELISDSFKVAIVRPPMIYGAHCPGNFQRLMQLSKRLPIIPNIN
  N315            PTTNYGISKKFAEQALQELISDSFKVAIVRPPMIYGAHCPGNFQRLMQLSKRLPIIPNIN
  NCTC8325        PTTNYGISKKFAEQALQELISDSFKVAIVRPPMIYGAHCPGNFQRLMQLSKRLPIIPNIN
  Newman          PTTNYGISKKFAEQALQELISDSFKVAIVRPPMIYGAHCPGNFQRLMQLSKRLPIIPNIN
  USA300_FPR3757  PTTNYGISKKFAEQALQELISDSFKVAIVRPPMIYGAHCPGNFQRLMQLSKRLPIIPNIN
                  ************************************************************
  
  COL             NQRSALYIKHLTAFIDQLISLEVTGVYHPQDSFYFDTSSVMYEIRRQSHRKTVLINMPSM
  N315            NQRSALYIKHLTAFIDQLISLEVTGVYHPQDSFYFDTSSVMYEIRRQSHRKTVLINMPSV
  NCTC8325        NQRSALYIKHLTAFIDQLISLEVTGVYHPQDSFYFDTSSVMYEIRRQSHRKTVLINMPSM
  Newman          NQRSALYIKHLTAFIDQLISLEVTGVYHPQDSFYFDTSSVMYEIRRQSHRKTVLINMPSM
  USA300_FPR3757  NQRSALYIKHLTAFIDQLISLEVTGVYHPQDSFYFDTSSVMYEIRRQSHRKTVLINMPSM
                  ***********************************************************:
  
  COL             LNKYFNKLSVFRKLFGNLIYSNTLYENNNALEIIPGKMSLVIADIMDETTTKDKA
  N315            LNKYFNKLSVFRKLFGNLTYSNTLYENNNALEVIPGKMSLVIADIMDETTTKDKA
  NCTC8325        LNKYFNKLSVFRKLFGNLIYSNTLYENNNALEIIPGKMSLVIADIMDETTTKDKA
  Newman          LNKYFNKLSVFRKLFGNLIYSNTLYENNNALEIIPGKMSLVIADIMDETTTKDKA
  USA300_FPR3757  LNKYFNKLSVFRKLFGNLIYSNTLYENNNALEIIPGKMSLVIADIMDETTTKDKA
                  ****************** *************:**********************