⊟Summary[edit | edit source]

pan ID^?: SAUPAN000980000
symbol^?: capF
synonym:
description^?: capsular polysaccharide biosynthesis protein Cap8F
- capsular polysaccharide biosynthesis protein Cap8F
- capsular polysaccharide biosynthesis protein Cap5F
- capsular polysaccharide synthesis enzyme CapF
- capsular polysaccharide biosynthesis protein CapF
- capsular polysaccharide synthesis protein Cap5F
- type 8 capsular polysaccharide synthesis protein Cap8F
- Capsular polysaccharide synthesis enzyme Cap8F
- capsular polysaccharide synthesis enzyme Cap5F
- NAD-dependent epimerase/dehydratase
- NAD dependent epimerase/dehydratase family protein
- UDP-2-acetamido-2,6-dideoxy-beta-L-talose 4-dehydrogenase
strand^?: +
coordinates^?: 1235629..1236744
synteny block^?: BlockID0005140
occurrence^?: in 100% of 34 strains

capF : capsule polysaccharide precursor UDP-N-acetyl-4-hexulose 4-ketoreductase/3-epimerase CapF ^[1]

• A crystal structure is available : 2ZKL

Staphylococci can produce up to 15 different capsules based on variants of the core trisaccharide N-acetyl-D-mannosaminouronate (ManNAcA) - N-acetyl-L-fucosamine (L-FucNAc) - N-acetyl-D-fucosamine (D-FucNAc). CapF catalyzes the second of three steps in the biosynthesis of UDP-N-acetyl-L-fucosamine (L-FucNAcA), a ketoreduction and C-3 epimerization of UDP-N-acetyl-2,6-dideoxy-beta-L-arabin-4-osamine to form UDP-N-acetyl-2,6-dideoxy-beta-L-talosamine.

Historically, different capsule types were assigned their own nomenclature (i.e. Cap5F and Cap8F for the two most prevalent capsule types, CP5 and CP8). The CapF protein is 98% identical between Cap5F and Cap8F, provides the same function, and is therefore harmonized as CapF.

⊟Orthologs[edit | edit source]

COL:

SACOL0141 (cap5F)

N315:

SA0149 (capF)

NCTC8325:

SAOUHSC_00119

Newman:

NWMN_0100 (capF)

USA300_FPR3757:

SAUSA300_0157 (cap5F)

04-02981:

SA2981_0155 (capF)

08BA02176:

C248_0143 (capF)

11819-97:

MS7_0148 (capF)

6850:

RSAU_000108 (capF)

71193:

ST398NM01_0168

ECT-R 2:

ECTR2_111

ED133:

SAOV_0101

ED98:

SAAV_0122 (cap5F)

HO 5096 0412:

SAEMRSA15_01200 (capF)

JH1:

SaurJH1_0145

JH9:

SaurJH9_0140

JKD6008:

SAA6008_00132 (cap5F)

JKD6159:

SAA6159_00138 (cap5F)

JSNZ:

JSNZ_000097 (cap8F)

LGA251:

SARLGA251_01280 (capF)

M013:

M013TW_0144

MRSA252:

SAR0156 (capF)

MSHR1132:

SAMSHR1132_01280

MSSA476:

SAS0129

Mu3:

SAHV_0153 (capF)

Mu50:

SAV0154 (capF)

MW2:

MW0129 (cap8F)

RF122:

SAB0095 (capF)

ST398:

SAPIG0168

T0131:

SAT0131_00144

TCH60:

HMPREF0772_10344 (cap5F)

TW20:

SATW20_01650 (capF)

USA300_TCH1516:

USA300HOU_0168 (cap5F)

VC40:

SAVC_00540

⊟Genome Viewer[edit | edit source]

⊟Alignments[edit | edit source]

alignment of orthologues:
CLUSTAL format alignment by MAFFT L-INS-i (v7.307)

COL             MNIVITGAKGFVGKNLKADLTSTTDHHIFEVHRQTKEEELESALLKADFVVHLAGVNRPE
N315            MNIVITGAKGFVGKNLKADLTSTTDHHIFEVHRQTKEEELESALLKADFIVHLAGVNRPE
NCTC8325        MNIVITGAKGFVGKNLKADLTSTTDHHIFEVHRQTKEEELESALLKADFVVHLAGVNRPE
Newman          MNIVITGAKGFVGKNLKADLTSTTDHHIFEVHRQTKEEELESALLKADFVVHLAGVNRPE
USA300_FPR3757  MNIVITGAKGFVGKNLKADLTSTTDHHIFEVHRQTKEEELESALLKADFVVHLAGVNRPE
                *************************************************:**********

COL             HDKEFSLGNVSYLDHVLDILTRNTKKPAILLSSSIQATQDNPYGESKLQGEQLLREYAEE
N315            HDKEFSLGNVSYLDHVLDILTRNTKKPAILLSSSIQATQDNPYGESKLQGEQLLREYAEE
NCTC8325        HDKEFSLGNVSYLDHVLDILTRNTKKPAILLSSSIQATQDNPYGESKLQGEQLLREYAEE
Newman          HDKEFSLGNVSYLDHVLDILTRNTKKPAILLSSSIQATQDNPYGESKLQGEQLLREYAEE
USA300_FPR3757  HDKEFSLGNVSYLDHVLDILTRNTKKPAILLSSSIQATQDNPYGESKLQGEQLLREYAEE
                ************************************************************

COL             YGNTVYIYRWPNLFGKWCKPNYNSVIATFCYKIARNEEIQVNDRNVELTLNYVDDIVAEI
N315            YGNTVYIYRWPNLFGKWCKPNYNSVIATFCYKIARNEEIQVNDRNVELTLNYVDDIVAEI
NCTC8325        YGNTVYIYRWPNLFGKWCKPNYNSVIATFCYKIARNEEIQVNDRNVELTLNYVDDIVAEI
Newman          YGNTVYIYRWPNLFGKWCKPNYNSVIATFCYKIARNEEIQVNDRNVELTLNYVDDIVAEI
USA300_FPR3757  YGNTVYIYRWPNLFGKWCKPNYNSVIATFCYKIARNEEIQVNDRNVELTLNYVDDIVAEI
                ************************************************************

COL             KRAIEGTPTIENGVPTVPNVFKVTLGEIVDLLYKFKQSRLDRTLPKLDNLFEKDLYSTYL
N315            KRAIEGTPTIENGVPTVPNVFKVTLGEIVDLLYKFKQSRLDRTLPKLDNLFEKDLYSTYL
NCTC8325        KRAIEGTPTIENGVPTVPNVFKVTLGEIVDLLYKFKQSRLDRTLPKLDNLFEKDLYSTYL
Newman          KRAIEGTPTIENGVPTVPNVFKVTLGEIVDLLYKFKQSRLDRTLPKLDNLFEKDLYSTYL
USA300_FPR3757  KRAIEGTPTIENGVPTVPNVFKVTLGEIVDLLYKFKQSRLDRTLPKLDNLFEKDLYSTYL
                ************************************************************

COL             SYLPSTDFSYPLLMNVDDRGSFTEFIKTPDRGQVSVNISKPGITKGNHWHHTKNEKFLVV
N315            SYLPSTDFSYPLLMNVDDRGSFTEFIKTPDRGQVSVNISKPGITKGNHWHHTKNEKFLVV
NCTC8325        SYLPSTDFSYPLLMNVDDRGSFTEFIKTPDRGQVSVNISKPGITKGNHWHHTKNEKFLVV
Newman          SYLPSTDFSYPLLMNVDDRGSFTEFIKTPDRGQVSVNISKPGITKGNHWHHTKNEKFLVV
USA300_FPR3757  SYLPSTDFSYPLLMNVDDRGSFTEFIKTPDRGQVSVNISKPGITKGNHWHHTKNEKFLVV
                ************************************************************

COL             SGKGVIRFRHVNDDEIIEYYVSGDKLEVVDIPVGYTHNIENLGDTDMVTIMWVNEMFDPN
N315            SGKGVIRFRHVNDDEIIEYYVSGDKLEVVDIPVGYTHNIENLGDTDMVTIMWVNEMFDPN
NCTC8325        SGKGVIRFRHVNDDEIIEYYVSGDKLEVVDIPVGYTHNIENLGDTDMVTIMWVNEMFDPN
Newman          SGKGVIRFRHVNDDEIIEYYVSGDKLEVVDIPVGYTHNIENLGDTDMVTIMWVNEMFDPN
USA300_FPR3757  SGKGVIRFRHVNDDEIIEYYVSGDKLEVVDIPVGYTHNIENLGDTDMVTIMWVNEMFDPN
                ************************************************************

COL             QPDTYFLEV
N315            QPDTYFLEV
NCTC8325        QPDTYFLEV
Newman          QPDTYFLEV
USA300_FPR3757  QPDTYFLEV
                *********

↑ Takamitsu Miyafusa, Jose M M Caaveiro, Yoshikazu Tanaka, Kouhei Tsumoto
Crystal structure of the enzyme CapF of Staphylococcus aureus reveals a unique architecture composed of two functional domains.
Biochem J: 2012, 443(3);671-80
[PubMed:22320426] [WorldCat.org] [DOI] (I p)Susan Boyle-Vavra, Xue Li, Md Tauqeer Alam, Timothy D Read, Julia Sieth, Colette Cywes-Bentley, Ginette Dobbins, Michael Z David, Neha Kumar, Samantha J Eells, Loren G Miller, David J Boxrud, Henry F Chambers, Ruth Lynfield, Jean C Lee, Robert S Daum
USA300 and USA500 clonal lineages of Staphylococcus aureus do not produce a capsular polysaccharide due to conserved mutations in the cap5 locus.
mBio: 2015, 6(2);
[PubMed:25852165] [WorldCat.org] [DOI] (I e)Satsawat Visansirikul, Stephen A Kolodziej, Alexei V Demchenko
Staphylococcus aureus capsular polysaccharides: a structural and synthetic perspective.
Org Biomol Chem: 2020, 18(5);783-798
[PubMed:31922180] [WorldCat.org] [DOI] (I p)

[1] Takamitsu Miyafusa, Jose M M Caaveiro, Yoshikazu Tanaka, Kouhei Tsumoto
Crystal structure of the enzyme CapF of Staphylococcus aureus reveals a unique architecture composed of two functional domains.
Biochem J: 2012, 443(3);671-80
[PubMed:22320426] [WorldCat.org] [DOI] (I p)Susan Boyle-Vavra, Xue Li, Md Tauqeer Alam, Timothy D Read, Julia Sieth, Colette Cywes-Bentley, Ginette Dobbins, Michael Z David, Neha Kumar, Samantha J Eells, Loren G Miller, David J Boxrud, Henry F Chambers, Ruth Lynfield, Jean C Lee, Robert S Daum
USA300 and USA500 clonal lineages of Staphylococcus aureus do not produce a capsular polysaccharide due to conserved mutations in the cap5 locus.
mBio: 2015, 6(2);
[PubMed:25852165] [WorldCat.org] [DOI] (I e)Satsawat Visansirikul, Stephen A Kolodziej, Alexei V Demchenko
Staphylococcus aureus capsular polysaccharides: a structural and synthetic perspective.
Org Biomol Chem: 2020, 18(5);783-798
[PubMed:31922180] [WorldCat.org] [DOI] (I p)

[1]

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Contents

⊟Summary[edit | edit source]

⊟Orthologs[edit | edit source]

⊟Genome Viewer[edit | edit source]

⊟Alignments[edit | edit source]

COL
N315
NCTC8325
Newman
USA300_FPR3757

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⊟Summary[edit | edit source]

⊟Orthologs[edit | edit source]

⊟Genome Viewer[edit | edit source]

⊟Alignments[edit | edit source]