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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02362
  • pan locus tag?: SAUPAN005413000
  • symbol: rho
  • pan gene symbol?: rho
  • synonym:
  • product: transcription termination factor Rho

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02362
  • symbol: rho
  • product: transcription termination factor Rho
  • replicon: chromosome
  • strand: -
  • coordinates: 2182974..2184290
  • length: 1317
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGCCTGAAAGAGAACGTACATCTCCTCAGTATGAATCATTCCACGAATTGTACAAGAAC
    TATACTACCAAGGAACTCACTCAAAAAGCTAAAACTCTTAAGTTGACGAACTATAGTAAA
    TTAAATAAAAAAGAACTTGTTCTAGCTATTATGGAAGCACAAATGGAAAAAGATGGTAAC
    TATTATATGGAAGGTATCTTAGATGATATACAACCAGATGGTTATGGTTTTTTAAGAACA
    GTGAACTATTCTAAAGGGGAAAAAGATATTTATATATCTGCTAGCCAAATTCGTCGTTTT
    GAAATTAAACGTGGGGATAAAGTAACTGGGAAAGTTAGAAAACCTAAAGATAACGAAAAA
    TATTATGGCTTATTACAAGTTGACTTTGTCAATGACCATAACGCAGAAGAAGTGAAGAAA
    CGTCCGCATTTCCAAGCTTTGACACCACTTTATCCAGATGAGCGTATTAAATTAGAGACA
    GAAATACAAAATTATTCAACGCGCATCATGGATTTAGTAACACCGATTGGTTTAGGTCAA
    CGTGGTTTAATAGTGGCGCCACCTAAAGCAGGTAAAACATCGTTATTAAAAGAAATAGCG
    AATGCAATCAGTACGAACAAACCAGATGCAAAGCTATTTATTTTGTTAGTTGGCGAGCGT
    CCTGAAGAGGTAACAGATTTAGAACGCTCAGTAGAAGCTGCTGAAGTCGTTCATTCAACG
    TTTGACGAACCACCAGAACACCATGTTAAAGTAGCTGAATTATTACTTGAACGTGCAAAG
    CGTTTAGTAGAAATTGGGGAAGATGTCATTATTTTAATGGATTCTATAACGAGATTAGCA
    CGCGCTTATAACTTAGTTATTCCACCAAGTGGTCGTACATTATCAGGTGGTTTAGATCCT
    GCATCTTTACACAAACCAAAAGCATTCTTCGGTGCAGCGAGAAATATTGAAGCGGGTGGA
    AGTTTAACAATACTTGCAACTGCATTAGTTGATACGGGTTCACGTATGGACGATATGATT
    TACGAAGAATTTAAAGGAACAGGTAACATGGAGTTACATTTAGATCGTAAATTGTCTGAA
    CGTCGTATCTTCCCTGCAATTGATATTGGCAGAAGTTCAACGCGTAAAGAAGAATTGTTG
    ATAAGTAAATCTGAATTAGACACATTATGGCAATTAAGAAATCTATTCACTGACTCAACT
    GACTTTACTGAAAGATTTATTCGCAAACTTAAAAGGTCTAAGAATAATGAAGATTTCTTC
    AAGCAGCTACAAAAGTCTGCAGAAGAAAGTACTAAAACGGGTCGACCTATAATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1317

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02362
  • symbol: Rho
  • description: transcription termination factor Rho
  • length: 438
  • theoretical pI: 8.53546
  • theoretical MW: 49969.8
  • GRAVY: -0.569406

⊟Function[edit | edit source]

  • reaction:
    EC 3.6.4.-?  ExPASy
  • TIGRFAM:
    Genetic information processing Transcription Transcription factors transcription termination factor Rho (TIGR00767; EC 3.6.4.-; HMM-score: 620.5)
    and 11 more
    Metabolism Energy metabolism ATP-proton motive force interconversion ATPase, FliI/YscN family (TIGR01026; EC 3.6.3.14; HMM-score: 85.1)
    Cellular processes Cellular processes Chemotaxis and motility flagellar protein export ATPase FliI (TIGR03496; EC 3.6.3.14; HMM-score: 84.7)
    flagellar protein export ATPase FliI (TIGR03498; EC 3.6.3.14; HMM-score: 84.3)
    Cellular processes Cellular processes Pathogenesis type III secretion apparatus H+-transporting two-sector ATPase (TIGR02546; EC 3.6.3.14; HMM-score: 84)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type III secretion apparatus H+-transporting two-sector ATPase (TIGR02546; EC 3.6.3.14; HMM-score: 84)
    Cellular processes Cellular processes Chemotaxis and motility flagellar protein export ATPase FliI (TIGR03497; EC 3.6.3.14; HMM-score: 74.7)
    Metabolism Energy metabolism ATP-proton motive force interconversion ATP synthase F1, beta subunit (TIGR01039; EC 3.6.3.14; HMM-score: 38.4)
    alternate F1F0 ATPase, F1 subunit alpha (TIGR03324; EC 3.6.3.-; HMM-score: 33)
    alternate F1F0 ATPase, F1 subunit beta (TIGR03305; EC 3.6.3.-; HMM-score: 27)
    Cellular processes Cellular processes Sporulation and germination stage III sporulation protein AA (TIGR02858; HMM-score: 25.3)
    Metabolism Energy metabolism ATP-proton motive force interconversion ATP synthase F1, alpha subunit (TIGR00962; EC 3.6.3.14; HMM-score: 19.9)
  • TheSEED  :
    • Transcription termination factor Rho
    RNA Metabolism Transcription Transcription factors bacterial  Transcription termination factor Rho
  • PFAM:
    OB (CL0021) Rho_RNA_bind; Rho termination factor, RNA-binding domain (PF07497; HMM-score: 98.1)
    and 9 more
    P-loop_NTPase (CL0023) ATP-synt_ab; ATP synthase alpha/beta family, nucleotide-binding domain (PF00006; HMM-score: 75.6)
    HeH (CL0306) Rho_N; Rho termination factor, N-terminal domain (PF07498; HMM-score: 44.3)
    P-loop_NTPase (CL0023) NACHT; NACHT domain (PF05729; HMM-score: 25.2)
    OB (CL0021) OB_RNB; Ribonuclease B OB domain (PF08206; HMM-score: 21.2)
    P-loop_NTPase (CL0023) AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 16)
    AAA_25; AAA domain (PF13481; HMM-score: 15.8)
    HTH (CL0123) Mor; Mor transcription activator family (PF08765; HMM-score: 14.8)
    P-loop_NTPase (CL0023) AAA_24; AAA domain (PF13479; HMM-score: 13.5)
    AAA_14; AAA domain (PF13173; HMM-score: 12.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002608
    • TAT(Tat/SPI): 0.000357
    • LIPO(Sec/SPII): 0.000255
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MPERERTSPQYESFHELYKNYTTKELTQKAKTLKLTNYSKLNKKELVLAIMEAQMEKDGNYYMEGILDDIQPDGYGFLRTVNYSKGEKDIYISASQIRRFEIKRGDKVTGKVRKPKDNEKYYGLLQVDFVNDHNAEEVKKRPHFQALTPLYPDERIKLETEIQNYSTRIMDLVTPIGLGQRGLIVAPPKAGKTSLLKEIANAISTNKPDAKLFILLVGERPEEVTDLERSVEAAEVVHSTFDEPPEHHVKVAELLLERAKRLVEIGEDVIILMDSITRLARAYNLVIPPSGRTLSGGLDPASLHKPKAFFGAARNIEAGGSLTILATALVDTGSRMDDMIYEEFKGTGNMELHLDRKLSERRIFPAIDIGRSSTRKEELLISKSELDTLWQLRNLFTDSTDFTERFIRKLKRSKNNEDFFKQLQKSAEESTKTGRPII

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

R S Washburn, A Marra, A P Bryant, M Rosenberg, D R Gentry
rho is not essential for viability or virulence in Staphylococcus aureus.
Antimicrob Agents Chemother: 2001, 45(4);1099-103
[PubMed:11257021] [WorldCat.org] [DOI] (P p)

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