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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02297
  • pan locus tag?: SAUPAN005331000
  • symbol: SAOUHSC_02297
  • pan gene symbol?: —
  • synonym:
  • product: S1 RNA-binding domain-containing protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02297
  • symbol: SAOUHSC_02297
  • product: S1 RNA-binding domain-containing protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2129345..2131495
  • length: 2151
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGACAATCAATTGATTAATTCAATCATAGAGAAATATCAATTTAGTAAAAAACAAATT
    GAAGCAGTATTAACACTGCTAGAAGAAAAAAATACAGTACCATTTATTGCGAGGTATCGA
    AAAGAGCAAACTGGTGGACTAGATGAAGTTCAAATAAAGCAAATTGATGACGAATACCAA
    TATATGGTCAATTTACAAAAACGTAAAGAAGAAGTTATCAAAAATATAGAACAGCAAGGA
    TTACTTACTGAGGAATTAAAGAAGGATATTTTAAAACAGAACAAATTACAACGTGTTGAA
    GACCTATATAGGCCTTTTAAACAAAAGAAAAAGACAAGGGCAACTGAGGCGAAACGTAAA
    GGGTTAGAGCCATTAGCGATATGGATGAAGGCACGTAAACATGAAGTCTCAATTGAAGAA
    AAAGCACAACAATTTATAAATGAAGAAGTGCAATCGGTTGAAGATGCTATCAAAGGTGCA
    CAAGATATTATTGCGGAACAAATTTCAGATAATCCTAAATATAGAACAAAAATTTTAAAA
    GATATGTATCATCAAGGTGTGTTAACTACATCTAAAAAGAAAAATGCTGAAGATGAAAAA
    GGTATTTTTGAAATGTACTATGCATATAGTGAGCCAATTAAACGCATTGCTAATCATAGA
    GTTTTAGCTGTTAATCGTGGTGAAAAAGAGAAAGTATTATCTGTAAAGTTTGAATTCGAT
    ACGACATCAGTAGAGGATTTCATTGCACGTCAAGAAATCAATCATAATAATGTAAATCGC
    AGTTATATTTTAGAGGCGATTAAAGATAGCTTGAAACGCTTAATTGTCCCTTCGATAGAG
    CGTGAAATCCATGCTGATTTAACTGAAAAAGCTGAAAATCATGCAATAGATGTTTTTAGT
    GAAAACTTAAGAAATCTATTACTGCAACCTCCAATGAAAGGTAAACAAATATTAGGCGTA
    GATCCAGCATTTAGAACAGGTTGTAAATTAGCAGTCATTAACCCATTCGGTACTTTTATA
    GCAAAAGGTGTGATTTATCCGCATCCACCAGTTTCTAAAAAAGAGGCAGCAGAGAAGGAT
    TTTGTACAAATGGTTAAAGCGTATGATGTGCAATTAATTGCAATTGGCAATGGTACTGCA
    AGTCGTGAAACAGAACAATTTGTTGCAGATTTAATTAAAAAGCATCAGTTGCCAGTACAA
    TTCATCATTGTCAATGAAGCGGGCGCTTCAGTATACTCAGCATCAGAAATTGCTAGAGAT
    GAATTTCCTGATTTTCAAGTGGAAGAAAGAAGTGCCGTATCAATTGGACGACGCGTTCAA
    GATCCATTAAGTGAATTAGTTAAAATCGATCCTAAATCTATTGGTGTCGGACAATACCAG
    CATGATGTTAATCAAAAAGCACTAGAAAATGCATTAACTTTCGTTGTTGAAACAGCAGTT
    AACCAAGTAGGTGTTGATGTCAATACAGCATCATCATCATTATTACAATATGTGTCTGGT
    CTAAGTTCGCAAATTGCTAAAAATATAATTGCATATAGAGAAGAAAATGGCGCAATAAAG
    CATAATAAAGAGTTAAGCAAGATTAAGCGTTTGGGTGCTAAAACTTTTGAGCAGAGTATA
    GGATTTTTAAGAATAGTAGACGGTTCAGAGCCATTGGATAATACATCGATTCATCCCGAA
    AGCTATAAAGTAACGTATCAGTTATTAGATAAACTCGGATTTGGTGGCAATGATTTAGGT
    AGTGATGCATTAAAAGCTAAATTAAATTCATTGGATATGGATGAACTGGCGATTGAATTA
    CAAGTCGGTGTACCTACATTAGAGGATATTATTAAATCCTTGAAAGCACCTAATCGAGAT
    CCAAGGGACGAGTTTGAAACACCAATCCTTAAATCCGATGTGCTATCAATTGAAGATTTA
    CAAGAAGGAATGAAATTGAGTGGTACAGTTAGAAATGTAGTAGATTTTGGAGCATTTGTA
    GATATAGGTGTTAAACAAGATGGACTTGTCCATGTTTCGAAACTTTCTAAGAAGTTCGTA
    AAAAATCCAATGGATATTGTCAGTGTTGGTGATATCGTTGACGTTTGGATTTACAGTATT
    GACAAAAATAAAGACAAAGTATCATTAACGATGATTGATCCACATGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2151

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02297
  • symbol: SAOUHSC_02297
  • description: S1 RNA-binding domain-containing protein
  • length: 716
  • theoretical pI: 6.24525
  • theoretical MW: 80933.9
  • GRAVY: -0.426397

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein bS1 (TIGR00717; HMM-score: 55.9)
    Genetic information processing Transcription Degradation of RNA polyribonucleotide nucleotidyltransferase (TIGR03591; EC 2.7.7.8; HMM-score: 49)
    and 5 more
    Cellular processes Cellular processes DNA transformation comEA protein (TIGR01259; HMM-score: 40.9)
    Cellular processes Cellular processes DNA transformation competence protein ComEA helix-hairpin-helix repeat region (TIGR00426; HMM-score: 36.6)
    guanosine pentaphosphate synthetase I/polyribonucleotide nucleotidyltransferase (TIGR02696; EC 2.7.-.-,2.7.7.8; HMM-score: 26)
    Genetic information processing Mobile and extrachromosomal element functions Prophage functions phage uncharacterized protein, C-terminal domain (TIGR01630; HMM-score: 16.1)
    Genetic information processing Transcription DNA-dependent RNA polymerase DNA-directed RNA polymerase (TIGR00448; EC 2.7.7.6; HMM-score: 15.4)
  • TheSEED  :
    • Transcription accessory protein (S1 RNA-binding domain)
    CBSS-393131.3.peg.612  Transcription accessory protein (S1 RNA-binding domain)
    and 1 more
    Cell division-ribosomal stress proteins cluster  Transcription accessory protein (S1 RNA-binding domain)
  • PFAM:
    no clan defined Tex_N; Tex-like protein N-terminal domain (PF09371; HMM-score: 216)
    YqgF (CL0580) Tex_YqgF; Tex protein YqgF-like domain (PF16921; HMM-score: 182)
    and 9 more
    HHH (CL0198) HHH_3; Helix-hairpin-helix motif (PF12836; HMM-score: 89.6)
    OB (CL0021) S1; S1 RNA binding domain (PF00575; HMM-score: 55.7)
    YqgF (CL0580) YqgF; Holliday-junction resolvase-like of SPT6 (PF14639; HMM-score: 33.1)
    HHH (CL0198) HHH_7; Helix-hairpin-helix motif (PF14635; HMM-score: 32.2)
    no clan defined DLD; Death-like domain of SPT6 (PF14878; HMM-score: 21.6)
    SRA1; Steroid receptor RNA activator (SRA1) (PF07304; HMM-score: 14.5)
    HHH (CL0198) HHH_6; Helix-hairpin-helix motif (PF14579; HMM-score: 12.9)
    HTH (CL0123) Sigma54_CBD; Sigma-54 factor, core binding domain (PF04963; HMM-score: 11.6)
    no clan defined HTH_44; Helix-turn-helix DNA-binding domain of SPT6 (PF14641; HMM-score: 10.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001492
    • TAT(Tat/SPI): 0.000112
    • LIPO(Sec/SPII): 0.000284
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MDNQLINSIIEKYQFSKKQIEAVLTLLEEKNTVPFIARYRKEQTGGLDEVQIKQIDDEYQYMVNLQKRKEEVIKNIEQQGLLTEELKKDILKQNKLQRVEDLYRPFKQKKKTRATEAKRKGLEPLAIWMKARKHEVSIEEKAQQFINEEVQSVEDAIKGAQDIIAEQISDNPKYRTKILKDMYHQGVLTTSKKKNAEDEKGIFEMYYAYSEPIKRIANHRVLAVNRGEKEKVLSVKFEFDTTSVEDFIARQEINHNNVNRSYILEAIKDSLKRLIVPSIEREIHADLTEKAENHAIDVFSENLRNLLLQPPMKGKQILGVDPAFRTGCKLAVINPFGTFIAKGVIYPHPPVSKKEAAEKDFVQMVKAYDVQLIAIGNGTASRETEQFVADLIKKHQLPVQFIIVNEAGASVYSASEIARDEFPDFQVEERSAVSIGRRVQDPLSELVKIDPKSIGVGQYQHDVNQKALENALTFVVETAVNQVGVDVNTASSSLLQYVSGLSSQIAKNIIAYREENGAIKHNKELSKIKRLGAKTFEQSIGFLRIVDGSEPLDNTSIHPESYKVTYQLLDKLGFGGNDLGSDALKAKLNSLDMDELAIELQVGVPTLEDIIKSLKAPNRDPRDEFETPILKSDVLSIEDLQEGMKLSGTVRNVVDFGAFVDIGVKQDGLVHVSKLSKKFVKNPMDIVSVGDIVDVWIYSIDKNKDKVSLTMIDPHE

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SAOUHSC_00520(rplJ)50S ribosomal protein L10  [3] (data from MRSA252)
    SAOUHSC_02477(rpsI)30S ribosomal protein S9  [3] (data from MRSA252)
    SAOUHSC_01040pyruvate dehydrogenase complex, E1 component subunit alpha  [3] (data from MRSA252)
    SAOUHSC_02969arginine deiminase  [3] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 3.2 3.3 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  4. ↑ 4.0 4.1 4.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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