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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01809
  • pan locus tag?: SAUPAN004348000
  • symbol: SAOUHSC_01809
  • pan gene symbol?: accD
  • synonym:
  • product: acetyl-CoA carboxylase carboxyltransferase subunit beta

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01809
  • symbol: SAOUHSC_01809
  • product: acetyl-CoA carboxylase carboxyltransferase subunit beta
  • replicon: chromosome
  • strand: -
  • coordinates: 1711926..1712783
  • length: 858
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGTTTAAAGATTTTTTTAATCGAACAAAGAAAAAGAAATATCTTACAGTACAAGACTCT
    AAAAATAATGATGTGCCTGCAGGTATTATGACTAAGTGTCCAAAGTGTAAGAAAATTATG
    TACACAAAAGAATTAGCTGAAAATTTAAATGTGTGCTTTAATTGTGATCATCATATTGCT
    TTAACTGCGTATAAACGTATAGAAGCAATTTCTGATGAAGGATCATTTACAGAATTCGAT
    AAGGGAATGACCTCTGCGAATCCATTAGATTTTCCAAGTTATTTAGAAAAAATTGAAAAG
    GACCAACAAAAGACAGGTCTTAAAGAAGCAGTTGTGACTGGTACAGCACAACTAGATGGT
    ATGAAATTTGGCGTTGCTGTCATGGATTCACGTTTTAGAATGGGAAGTATGGGATCGGTT
    ATCGGTGAAAAGATATGTCGCATCATTGATTACTGCACTGAGAACCGTTTACCATTTATT
    CTTTTCTCTGCAAGTGGTGGTGCACGTATGCAAGAAGGTATTATTTCCTTGATGCAAATG
    GGTAAAACCAGTGTATCTTTAAAACGTCATTCTGACGCTGGACTATTATATATATCATAT
    TTAACACATCCAACTACTGGTGGTGTATCTGCAAGTTTTGCATCAGTTGGTGATATAAAT
    TTAAGTGAGCCAAAAGCGTTGATAGGTTTTGCAGGTCGTCGAGTTATTGAACAGACAATA
    AACGAAAAATTGCCAGATGATTTCCAAACTGCAGAATTTTTATTAGAGCATGGACAATTG
    GATAAAGTTGTACATCGTAATGATATGCGTCAAACATTGTCTGAAATTCTAAAAATCCAT
    CAAGAGGTGACTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    858

⊟Protein[edit | edit source]

Protein Data Bank: 2F9I
Protein Data Bank: 5KDR

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01809
  • symbol: SAOUHSC_01809
  • description: acetyl-CoA carboxylase carboxyltransferase subunit beta
  • length: 285
  • theoretical pI: 8.35802
  • theoretical MW: 31871.6
  • GRAVY: -0.302105

⊟Function[edit | edit source]

  • reaction:
    EC 6.4.1.2?  ExPASy
    Acetyl-CoA carboxylase ATP + acetyl-CoA + HCO3(-) = ADP + phosphate + malonyl-CoA
  • TIGRFAM:
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis acetyl-CoA carboxylase, carboxyl transferase, beta subunit (TIGR00515; EC 6.4.1.2; HMM-score: 415.1)
    and 4 more
    Metabolism Transport and binding proteins Cations and iron carrying compounds methylmalonyl-CoA decarboxylase alpha subunit (TIGR01117; EC 4.1.1.41; HMM-score: 52.4)
    Metabolism Energy metabolism Fermentation methylmalonyl-CoA decarboxylase alpha subunit (TIGR01117; EC 4.1.1.41; HMM-score: 52.4)
    Metabolism Energy metabolism ATP-proton motive force interconversion methylmalonyl-CoA decarboxylase alpha subunit (TIGR01117; EC 4.1.1.41; HMM-score: 52.4)
    biotin-independent malonate decarboxylase, beta subunit (TIGR03133; EC 4.1.1.88; HMM-score: 46.5)
  • TheSEED  :
    • Acetyl-coenzyme A carboxyl transferase beta chain (EC 6.4.1.2)
    Fatty Acids, Lipids, and Isoprenoids Fatty acids Fatty Acid Biosynthesis FASII  Acetyl-coenzyme A carboxyl transferase beta chain (EC 6.4.1.2)
    and 1 more
    Virulence, Disease and Defense Bacteriocins, ribosomally synthesized antibacterial peptides Colicin V and Bacteriocin Production Cluster  Acetyl-coenzyme A carboxyl transferase beta chain (EC 6.4.1.2)
  • PFAM:
    ClpP_crotonase (CL0127) Carboxyl_trans; Carboxyl transferase domain (PF01039; HMM-score: 66.9)
    and 2 more
    AB_hydrolase (CL0028) FSH1; Serine hydrolase (FSH1) (PF03959; HMM-score: 12)
    Zn_Beta_Ribbon (CL0167) RNA_POL_M_15KD; RNA polymerases M/15 Kd subunit (PF02150; HMM-score: 9.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.054236
    • TAT(Tat/SPI): 0.001063
    • LIPO(Sec/SPII): 0.003453
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MFKDFFNRTKKKKYLTVQDSKNNDVPAGIMTKCPKCKKIMYTKELAENLNVCFNCDHHIALTAYKRIEAISDEGSFTEFDKGMTSANPLDFPSYLEKIEKDQQKTGLKEAVVTGTAQLDGMKFGVAVMDSRFRMGSMGSVIGEKICRIIDYCTENRLPFILFSASGGARMQEGIISLMQMGKTSVSLKRHSDAGLLYISYLTHPTTGGVSASFASVGDINLSEPKALIGFAGRRVIEQTINEKLPDDFQTAEFLLEHGQLDKVVHRNDMRQTLSEILKIHQEVTK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [2] [3]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. ↑ 4.0 4.1 4.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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