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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00912
  • pan locus tag?: SAUPAN003113000
  • symbol: SAOUHSC_00912
  • pan gene symbol?: clpB
  • synonym:
  • product: ATP-dependent Clp protease ATP-binding subunit ClpB

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00912
  • symbol: SAOUHSC_00912
  • product: ATP-dependent Clp protease ATP-binding subunit ClpB
  • replicon: chromosome
  • strand: +
  • coordinates: 882636..885245
  • length: 2610
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGATATAAATAAAATGACATATGCTGTTCAAAGTGCTTTACAACAAGCAGTTGAACTG
    AGTCAGCAACATAAATTACAAAATATAGAAATTGAGGCAATTTTAAGCGCTGCCTTAAAT
    GAAAGTGAAAGCTTATATAAAAGTATTTTAGAACGAGTAAATATTGAGGTAGATCAATTA
    AACAAAGCTTATGAAGACAAACTAAACACGTATGCATCTGTAGAAGGTGACAATATACAA
    TATGGTCAATATATTAGCCAACAAGCAAACCAATTGATAACTAAGGCTGAATCATACATG
    AAAGAATATGAAGATGAATATATTTCAATGGAGCATATTTTACGTTCGGCAATGGACATT
    GATCAAACAACAAAACATTATATAAATAATAAAGTAGAAGTTATCAAAGAAATTATTAAA
    AAAGTAAGAGGGGGAAATCACGTGACATCACAAAATCCAGAAGTTAATTACGAAGCATTA
    GCTAAATATGGCCGCGACTTAGTAGAAGAAGTTAGACAAGGTAAAATGGATCCTGTTATA
    GGAAGAGATGAAGAAATTCGAAATACGATTCGTATTTTAAGTCGTAAAACTAAAAACAAC
    CCTGTGCTCATTGGTGAACCAGGTGTTGGTAAAACTGCAATTGTTGAAGGATTAGCGCAA
    CGTATAGTTAAGAAAGATGTGCCAGAATCATTATTAGATAAAACTGTTTTTGAGTTAGAT
    TTAAGCGCATTAGTAGCGGGCGCTAAATATCGTGGTGAATTTGAAGAGAGATTAAAAGCA
    GTCCTAAAAGAAGTTAAAGAGTCTGATGGTAGAATTATATTATTTATTGATGAAATCCAT
    ATGCTTGTAGGTGCTGGTAAAACAGATGGTGCCATGGATGCAGGCAACATGCTAAAACCA
    ATGTTAGCACGAGGAGAGTTACATTGTATTGGTGCAACAACTTTAAATGAATATCGAGAA
    TATATTGAAAAAGATTCGGCATTAGAGCGTCGTTTCCAAAAAGTAGCAGTTAGTGAGCCT
    GATGTTGAAGATACAATTTCAATTTTACGTGGTTTAAAAGAACGATATGAAGTGTATCAT
    GGTGTGCGTATTCAAGATAGAGCCTTAGTTGCTGCCGCTGAATTGTCTGATCGTTACATC
    ACTGATCGTTTTTTACCAGATAAAGCGATTGATTTAGTTGACCAAGCATGTGCAACAATT
    CGTACGGAAATGGGATCAAATCCAACTGAATTGGATCAAGTTAATAGACGTGTCATGCAA
    TTAGAAATTGAAGAAAGCGCACTTAAAAATGAATCTGACAATGCGAGCAAACAGAGATTA
    CAAGAACTACAAGAAGAGCTTGCCAATGAAAAAGAGAAACAAGCAGCACTTCAATCTCGT
    GTAGAATCAGAAAAAGAAAAAATAGCAAATTTACAAGAAAAACGTGCGCAACTAGATGAA
    AGTAGACAAGCGTTGGAAGATGCACAAACAAATAACAATTTAGAAAAAGCTGCTGAACTA
    CAATATGGAACAATTCCTCAATTGGAAAAAGAACTTAGAGAATTAGAGGATAATTTCCAA
    GATGAGCAAGGTGAAGATACAGATCGAATGATTCGTGAAGTTGTAACAGACGAAGAAATT
    GGCGATATTGTCAGCCAATGGACAGGCATACCAGTTTCAAAATTAGTTGAAACAGAACGT
    GAAAAATTACTTCACTTAAGTGACATCTTGCATAAACGTGTTGTAGGTCAAGATAAAGCG
    GTTGACCTGGTTTCAGATGCAGTAGTTAGAGCAAGAGCAGGTATTAAAGATCCAAACAGA
    CCTATTGGTAGTTTCTTATTCCTAGGTCCAACTGGAGTAGGTAAAACTGAATTAGCTAAA
    TCATTAGCTGCATCATTATTTGATTCTGAAAAACATATGATTCGTATTGATATGAGTGAA
    TATATGGAAAAACATGCAGTATCAAGATTGATAGGGGCACCTCCAGGATATATTGGACAT
    GATGAAGGGGGTCAATTAACTGAAGCGGTTCGTCGTAATCCATACTCAGTTATTTTATTA
    GATGAGGTTGAAAAAGCGCATACTGACGTCTTTAATGTATTATTGCAAATTTTAGATGAA
    GGCCGTTTAACTGATTCTAAAGGACGTAGCGTTGATTTTAAAAATACTATTATTATTATG
    ACAAGTAATATTGGATCTCAAGTTTTATTAGAAAACGTAAAAGAGACTGGTGAAATTACA
    GAATCAACAGAAAAAGCTGTTATGACAAGTTTAAATGCATATTTCAAACCAGAAATTTTG
    AATCGTATGGATGATATCGTATTATTTAAACCATTATCTATTGATGACATGAGTATGATT
    GTAGATAAAATCTTAACGCAATTAAATATAAGATTATTAGAACAACGAATCTCAATTGAA
    GTTTCTGATGATGCTAAAGCTTGGCTAGGTCAAGAAGCTTATGAACCTCAATACGGTGCA
    AGACCATTAAAACGTTTTGTACAACGCCAAATTGAAACACCATTAGCACGTATGATGATT
    AAAGAGGGATTCCCAGAAGGTACAACGATTAAAGTTAATTTAAATTCAGACAATAACTTA
    ACGTTTAATGTTGAAAAAATTCATGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2610

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00912
  • symbol: SAOUHSC_00912
  • description: ATP-dependent Clp protease ATP-binding subunit ClpB
  • length: 869
  • theoretical pI: 4.68338
  • theoretical MW: 98330.8
  • GRAVY: -0.485731

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Protein folding and stabilization ATP-dependent chaperone protein ClpB (TIGR03346; HMM-score: 1276.6)
    and 16 more
    Cellular processes Cellular processes Pathogenesis type VI secretion ATPase, ClpV1 family (TIGR03345; HMM-score: 802.2)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type VI secretion ATPase, ClpV1 family (TIGR03345; HMM-score: 802.2)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides ATP-dependent Clp protease ATP-binding subunit ClpA (TIGR02639; HMM-score: 779.2)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair Holliday junction DNA helicase RuvB (TIGR00635; EC 3.6.4.12; HMM-score: 23.9)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DnaA regulatory inactivator Hda (TIGR03420; HMM-score: 21.4)
    Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02169; HMM-score: 20.4)
    Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02169; HMM-score: 20.4)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, delta' subunit (TIGR00678; EC 2.7.7.7; HMM-score: 20.3)
    Cellular processes Cellular processes Other gas vesicle protein GvpN (TIGR02640; HMM-score: 19.4)
    Cellular processes Cellular processes Sporulation and germination ATP-dependent protease LonB (TIGR02902; EC 3.4.21.-; HMM-score: 17.7)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides ATP-dependent protease LonB (TIGR02902; EC 3.4.21.-; HMM-score: 17.7)
    Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02168; HMM-score: 17.5)
    Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02168; HMM-score: 17.5)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, subunit gamma and tau (TIGR02397; EC 2.7.7.7; HMM-score: 16.9)
    Cellular processes Cellular processes Chemotaxis and motility flagellar biosynthesis protein FlhF (TIGR03499; HMM-score: 15.3)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis adenylosuccinate synthase (TIGR00184; EC 6.3.4.4; HMM-score: 9.7)
  • TheSEED  :
    • ClpB protein
    Protein Metabolism Protein degradation Proteolysis in bacteria, ATP-dependent  ClpB protein
    and 1 more
    Protein Metabolism Protein folding Protein chaperones  ClpB protein
  • PFAM:
    P-loop_NTPase (CL0023) AAA_2; AAA domain (Cdc48 subfamily) (PF07724; HMM-score: 187.1)
    and 39 more
    AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 94.9)
    no clan defined ClpB_D2-small; C-terminal, D2-small domain, of ClpB protein (PF10431; HMM-score: 84)
    P-loop_NTPase (CL0023) AAA_5; AAA domain (dynein-related subfamily) (PF07728; HMM-score: 75.8)
    no clan defined Clp_N; Clp amino terminal domain, pathogenicity island component (PF02861; HMM-score: 49.6)
    P-loop_NTPase (CL0023) AAA_16; AAA ATPase domain (PF13191; HMM-score: 46)
    Sigma54_activat; Sigma-54 interaction domain (PF00158; HMM-score: 43.2)
    AAA_14; AAA domain (PF13173; HMM-score: 30.3)
    AAA_22; AAA domain (PF13401; HMM-score: 30.2)
    IstB_IS21; IstB-like ATP binding protein (PF01695; HMM-score: 30)
    AAA_18; AAA domain (PF13238; HMM-score: 26.8)
    DUF815; Protein of unknown function (DUF815) (PF05673; HMM-score: 24.9)
    ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 24.8)
    Mg_chelatase; Magnesium chelatase, subunit ChlI (PF01078; HMM-score: 24.4)
    RuvB_N; Holliday junction DNA helicase ruvB N-terminus (PF05496; HMM-score: 24)
    Roc; Ras of Complex, Roc, domain of DAPkinase (PF08477; HMM-score: 23.5)
    NACHT; NACHT domain (PF05729; HMM-score: 23.4)
    RNA_helicase; RNA helicase (PF00910; HMM-score: 23.2)
    DNA_pol3_delta2; DNA polymerase III, delta subunit (PF13177; HMM-score: 21.5)
    AAA_24; AAA domain (PF13479; HMM-score: 20.9)
    RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 20.6)
    AAA_33; AAA domain (PF13671; HMM-score: 20.3)
    AAA_3; ATPase family associated with various cellular activities (AAA) (PF07726; HMM-score: 19.7)
    AAA_25; AAA domain (PF13481; HMM-score: 18.8)
    AAA_28; AAA domain (PF13521; HMM-score: 18.8)
    TsaE; Threonylcarbamoyl adenosine biosynthesis protein TsaE (PF02367; HMM-score: 18.7)
    CbiA; CobQ/CobB/MinD/ParA nucleotide binding domain (PF01656; HMM-score: 18.3)
    Zeta_toxin; Zeta toxin (PF06414; HMM-score: 18.2)
    NTPase_1; NTPase (PF03266; HMM-score: 16.8)
    SRP54; SRP54-type protein, GTPase domain (PF00448; HMM-score: 14.7)
    AAA_19; AAA domain (PF13245; HMM-score: 14.4)
    AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 12.8)
    Reo_sigma (CL0326) Reo_sigmaC; Reovirus sigma C capsid protein (PF04582; HMM-score: 12.7)
    no clan defined DUF3217; Protein of unknown function (DUF3217) (PF11506; HMM-score: 11.8)
    P-loop_NTPase (CL0023) DUF87; Domain of unknown function DUF87 (PF01935; HMM-score: 11.6)
    ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 11.4)
    PhoH; PhoH-like protein (PF02562; HMM-score: 10.6)
    AAA_23; AAA domain (PF13476; HMM-score: 10.2)
    ABC_tran; ABC transporter (PF00005; HMM-score: 10)
    no clan defined Exonuc_VII_L; Exonuclease VII, large subunit (PF02601; HMM-score: 6.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003194
    • TAT(Tat/SPI): 0.000293
    • LIPO(Sec/SPII): 0.000435
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MDINKMTYAVQSALQQAVELSQQHKLQNIEIEAILSAALNESESLYKSILERVNIEVDQLNKAYEDKLNTYASVEGDNIQYGQYISQQANQLITKAESYMKEYEDEYISMEHILRSAMDIDQTTKHYINNKVEVIKEIIKKVRGGNHVTSQNPEVNYEALAKYGRDLVEEVRQGKMDPVIGRDEEIRNTIRILSRKTKNNPVLIGEPGVGKTAIVEGLAQRIVKKDVPESLLDKTVFELDLSALVAGAKYRGEFEERLKAVLKEVKESDGRIILFIDEIHMLVGAGKTDGAMDAGNMLKPMLARGELHCIGATTLNEYREYIEKDSALERRFQKVAVSEPDVEDTISILRGLKERYEVYHGVRIQDRALVAAAELSDRYITDRFLPDKAIDLVDQACATIRTEMGSNPTELDQVNRRVMQLEIEESALKNESDNASKQRLQELQEELANEKEKQAALQSRVESEKEKIANLQEKRAQLDESRQALEDAQTNNNLEKAAELQYGTIPQLEKELRELEDNFQDEQGEDTDRMIREVVTDEEIGDIVSQWTGIPVSKLVETEREKLLHLSDILHKRVVGQDKAVDLVSDAVVRARAGIKDPNRPIGSFLFLGPTGVGKTELAKSLAASLFDSEKHMIRIDMSEYMEKHAVSRLIGAPPGYIGHDEGGQLTEAVRRNPYSVILLDEVEKAHTDVFNVLLQILDEGRLTDSKGRSVDFKNTIIIMTSNIGSQVLLENVKETGEITESTEKAVMTSLNAYFKPEILNRMDDIVLFKPLSIDDMSMIVDKILTQLNIRLLEQRISIEVSDDAKAWLGQEAYEPQYGARPLKRFVQRQIETPLARMMIKEGFPEGTTIKVNLNSDNNLTFNVEKIHE

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SAOUHSC_02820hypothetical protein  [3] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CtsR* (repression) regulon
    CtsR*(TF)important in Heat shock response; compare RegPrecise for N315  [4]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  4. ↑ 4.0 4.1 4.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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