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NCBI: 03-AUG-2016
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus NCTC8325
- locus tag: SAOUHSC_00904
- pan locus tag?: SAUPAN003076000
- symbol: SAOUHSC_00904
- pan gene symbol?: addB
- synonym:
- product: exonuclease RexB
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAOUHSC_00904
- symbol: SAOUHSC_00904
- product: exonuclease RexB
- replicon: chromosome
- strand: +
- coordinates: 868371..871847
- length: 3477
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3921750 NCBI
- RefSeq: YP_499457 NCBI
- BioCyc: G1I0R-847 BioCyc
- MicrobesOnline: 1289368 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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3421ATGACATTACATGCTTATTTAGGTAGAGCGGGAACAGGTAAGTCTACGAAAATGTTGACC
GAAATAAAACAAAAAATGAAAGCAGATCCGCTTGGAGATCCAATCATTTTAATTGCGCCA
ACTCAAAGTACATTTCAATTAGAACAAGCCTTTGTCAATGATCCGGAATTAAATGGTAGT
TTAAGAACAGAAGTGTTGCATTTTGAACGATTAAGTCATCGTATTTTCCAAGAAGTTGGT
AGTTATAGCGAACAAAAGTTATCTAAAGCTGCAACGGAAATGATGATTTATAACATTGTT
CAAGAACAACAAAAGTATTTAAAACTTTATCAATCACAAGCAAAATATTATGGGTTTAGT
GAAAAATTAACAGAACAAATTCAAGATTTTAAAAAATATGCAGTAACGCCTGAACATTTA
GAACACTTTATTGCTGATAAAAATATGCAAACTCGAACTAAAAATAAGTTAGAGGATATT
GCTTTAATATACCGTGAGTTCGAACAACGCATTCAAAACGAGTTTATTACGGGTGAGGAT
TCATTACAATATTTTATTGATTGTATGCCGAAATCAGAGTGGCTAAAACGTGCTGATATA
TATATTGATGGTTTTCACAACTTTTCAACGATTGAGTATTTAATAATCAAAGGATTAATT
AAATATGCGAAGAGTGTCACAATTATATTGACGACAGATGGTAACCACGATCAATTTAGT
TTATTTAGAAAACCATCGGAAGTGTTACGACATATTGAAGAAATAGCAAATGAACTCAAT
ATTTCTATTGAACGTCAATATTTCAACCAATTATATCGCTTCAATAATCAAGATTTAAAG
CATCTTGAACAAGAATTTGATGCACTTCAAATCAATCGAGTGGCATGTCAAGGTCATATC
AATATTTTAGAATCTGCGACTATGAGAGAGGAAATAAATGAAATTGCGCGACGTATCATC
GTTGATATTCGTGATAAGCAATTACGATATCAAGATATTGCTATTTTATATCGTGATGAA
TCTTATGCTTATTTATTTGATTCCATATTACCGCTTTATAATATTCCTTATAATATTGAT
ACAAAGCGTTCGATGACACATCATCCGGTCATGGAAATGATTCGTTCATTGATTGAAGTT
ATTCAATCTAATTGGCAAGTGAATCCAATGCTACGCTTATTGAAGACTGATGTGTTAACG
GCATCATATCTAAAAAGTGCATACTTAGTTGATTTACTTGAAAATTTTGTACTTGAACGT
GGTATATACGGTAAACGTTGGTTAGATGATGAGCTATTTAATGTCGAACATTTTAGCAAA
ATGGGGCGTAAAGCGCATAAACTGACCGAAGATGAACGTAACACATTTGAACAAGTCGTT
AAGTTAAAGAAAGATGTCATTGATAAAATTTTACATTTTGAAAAGCAAATGTCACAAGCG
GAAACTGTAAAAGATTTTGCAACTGCTTTTTATGAAAGTATGGAATATTTCGAACTGCCA
AATCAATTGATGACAGAGCGAGATGAACTTGATTTAAATGGTAATCATGAAAAGGCGGAG
GAAATTGATCAAATATGGAATGGCTTAATTCAAATCCTTGATGACTTAGTTCTAGTATTT
GGAGATGAACCAATGTCGATGGAACGTTTCTTAGAAGTATTTGATATTGGTTTAGAACAA
TTAGAATTTGTTATGATTCCGCAAACATTGGACCAAGTAAGTATTGGTACGATGGATTTG
GCTAAAGTCGATAATAAGCAACATGTTTACTTAGTAGGTATGAATGATGGAACGATGCCA
CAACCAGTAACTGCGTCAAGCTTGATTACAGATGAAGAAAAGAAATACTTTGAACAGCAG
GCTAATGTCGAGTTAAGTCCAACATCAGATATTTTACAGATGGATGAAGCATTTGTTTGT
TATGTTGCTATGACTAGAGCTAAGGGAGATGTTACATTTTCTTACAGTCTAATGGGATCA
AGTGGTGATGATAAGGAGATCAGCCCATTTTTAAATCAAATTCAATCATTGTTCAACCAA
TTGGAAATTACTAACATTCCTCAATACCATGAAGTTAACCCATTGTCACTAATGCAACAT
GCTAAGCAAACCAAAATTACATTATTTGAAGCATTGCGTGCTTGGTTATATGATGAAATT
GTGGCTGATAGTTGGTTAGATGCTTATCAAGTAATTAGAGATAGCGATCATTTAAATCAA
GGTTTAGATTATTTAATGTCAGCATTAACGTTTGACAATGAAACTGTAAAATTAGGTGAA
ACGTTGTCTAAAGATTTATATGGTAAGGAAATCAATGCCAGTGTATCCCGTTTTGAAGGT
TATCAACAATGCCCATTTAAACACTATGCGTCACATGGTCTGAAACTAAATGAGCGAACG
AAGTATGAACTTCAAAACTTTGATTTAGGTGATATTTTCCATTCTGTTTTAAAATATATA
TCTGAACGTATTAATGGCGATTTTAAACAATTAGACCTGAAAAAAATAAGACAATTAACG
AATGAAGCATTGGAAGAAATTTTACCTAAAGTTCAGTTTAATTTATTAAATTCTTCAGCT
TACTATCGTTATTTATCAAGACGCATTGGCGCTATTGTAGAAACAACACTAAGCGCATTA
AAATATCAAGGCACGTATTCAAAGTTTATGCCAAAACATTTTGAGACAAGTTTTAGAAGG
AAACCAAGAACAAATGACGAATTAATTGCACAAACATTAACGACAACTCAAGGTATTCCA
ATTAATATTAGAGGGCAAATTGACCGTATCGATACGTATACAAAGAATGATACAAGTTTT
GTTAATATCATTGACTATAAATCCTCTGAAGGTAGTGCGACACTTGATTTAACGAAAGTA
TATTATGGTATGCAAATGCAAATGATGACATACATGGATATCGTTTTACAAAATAAACAA
CGCCTTGGATTAACAGATATTGTGAAACCAGGTGGATTATTATACTTCCATGTACATGAA
CCTAGAATTAAATTTAAATCATGGTCTGATATTGATGAAGATAAACTAGAACAAGATTTA
ATTAAAAAGTTTAAGTTGAGTGGTTTAGTTAATGCAGACCAAACTGTTATTGATGCATTG
GATATTCGTTTAGAACCTAAATTCACTTCAGATATTGTACCAGTTGGTTTGAATAAAGAT
GGCTCTTTGAGTAAACGAGGCAGCCAAGTGGCAGATGAAGCAACGATTTATAAATTCATC
CAACATAACAAAGAGAATTTTATAGAAACAGCTTCAAATATTATGGATGGACATACTGAA
GTTGCACCATTAAAGTACAAACAAAAATTGCCATGTGCTTTTTGTAGTTATCAATCGGTA
TGTCATGTAGATGGCATGATTGATAGTAAGCGATATCGAACTGTAGATGAAACAATAAAT
CCAATTGAAGCAATTCAAAATATTAACATTAATGATGAATTTGGGGGTGAGCAATAG60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAOUHSC_00904
- symbol: SAOUHSC_00904
- description: exonuclease RexB
- length: 1158
- theoretical pI: 5.06875
- theoretical MW: 134504
- GRAVY: -0.421675
⊟Function[edit | edit source]
- reaction: EC 3.6.4.12? ExPASyDNA helicase ATP + H2O = ADP + phosphateEC 3.1.-.-? ExPASy
- TIGRFAM: DNA metabolism DNA replication, recombination, and repair helicase-exonuclease AddAB, AddB subunit (TIGR02773; EC 3.1.-.-,3.6.4.12; HMM-score: 1416.8)and 4 moreDNA metabolism DNA replication, recombination, and repair ATP-dependent nuclease subunit B (TIGR02774; HMM-score: 173)probable DNA repair protein (TIGR03623; HMM-score: 32.5)DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase Rep (TIGR01074; EC 3.6.4.12; HMM-score: 31.5)DNA metabolism DNA replication, recombination, and repair double-strand break repair protein AddB (TIGR02786; HMM-score: 31.1)
- TheSEED :
- ATP-dependent nuclease, subunit B
- PFAM: PDDEXK (CL0236) PDDEXK_1; PD-(D/E)XK nuclease superfamily (PF12705; HMM-score: 174.6)and 3 moreP-loop_NTPase (CL0023) UvrD_C; UvrD-like helicase C-terminal domain (PF13361; HMM-score: 58.9)AAA_30; AAA domain (PF13604; HMM-score: 14.6)no clan defined Imm21; Immunity protein 21 (PF15589; HMM-score: 13)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors: Mg2+, [4Fe-4S] cluster
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.002995
- TAT(Tat/SPI): 0.000315
- LIPO(Sec/SPII): 0.00049
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MTLHAYLGRAGTGKSTKMLTEIKQKMKADPLGDPIILIAPTQSTFQLEQAFVNDPELNGSLRTEVLHFERLSHRIFQEVGSYSEQKLSKAATEMMIYNIVQEQQKYLKLYQSQAKYYGFSEKLTEQIQDFKKYAVTPEHLEHFIADKNMQTRTKNKLEDIALIYREFEQRIQNEFITGEDSLQYFIDCMPKSEWLKRADIYIDGFHNFSTIEYLIIKGLIKYAKSVTIILTTDGNHDQFSLFRKPSEVLRHIEEIANELNISIERQYFNQLYRFNNQDLKHLEQEFDALQINRVACQGHINILESATMREEINEIARRIIVDIRDKQLRYQDIAILYRDESYAYLFDSILPLYNIPYNIDTKRSMTHHPVMEMIRSLIEVIQSNWQVNPMLRLLKTDVLTASYLKSAYLVDLLENFVLERGIYGKRWLDDELFNVEHFSKMGRKAHKLTEDERNTFEQVVKLKKDVIDKILHFEKQMSQAETVKDFATAFYESMEYFELPNQLMTERDELDLNGNHEKAEEIDQIWNGLIQILDDLVLVFGDEPMSMERFLEVFDIGLEQLEFVMIPQTLDQVSIGTMDLAKVDNKQHVYLVGMNDGTMPQPVTASSLITDEEKKYFEQQANVELSPTSDILQMDEAFVCYVAMTRAKGDVTFSYSLMGSSGDDKEISPFLNQIQSLFNQLEITNIPQYHEVNPLSLMQHAKQTKITLFEALRAWLYDEIVADSWLDAYQVIRDSDHLNQGLDYLMSALTFDNETVKLGETLSKDLYGKEINASVSRFEGYQQCPFKHYASHGLKLNERTKYELQNFDLGDIFHSVLKYISERINGDFKQLDLKKIRQLTNEALEEILPKVQFNLLNSSAYYRYLSRRIGAIVETTLSALKYQGTYSKFMPKHFETSFRRKPRTNDELIAQTLTTTQGIPINIRGQIDRIDTYTKNDTSFVNIIDYKSSEGSATLDLTKVYYGMQMQMMTYMDIVLQNKQRLGLTDIVKPGGLLYFHVHEPRIKFKSWSDIDEDKLEQDLIKKFKLSGLVNADQTVIDALDIRLEPKFTSDIVPVGLNKDGSLSKRGSQVADEATIYKFIQHNKENFIETASNIMDGHTEVAPLKYKQKLPCAFCSYQSVCHVDGMIDSKRYRTVDETINPIEAIQNININDEFGGEQ
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas [1] [2]
- protein localization: data available for COL
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: [3] Multi-gene expression profiles
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
Proteomics: 2015, 15(21);3648-61
[PubMed:26224020] [WorldCat.org] [DOI] (I p) - ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
Sci Rep: 2017, 7(1);9718
[PubMed:28887440] [WorldCat.org] [DOI] (I e) - ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
PLoS Genet: 2016, 12(4);e1005962
[PubMed:27035918] [WorldCat.org] [DOI] (I e)