PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS14075 [old locus tag: SACOL2689 ]
• pan locus tag^?: SAUPAN006411000
• symbol: SACOL_RS14075
• pan gene symbol^?: icaA
• synonym:
• product: poly-beta-1,6 N-acetyl-D-glucosamine synthase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS14075 [old locus tag: SACOL2689 ]
• symbol: SACOL_RS14075
• product: poly-beta-1,6 N-acetyl-D-glucosamine synthase
• replicon: chromosome
• strand: +
• coordinates: 2763320..2764558
• length: 1239
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (2763320..2764558) NCBI
• BioCyc: SACOL_RS14075 BioCyc
• MicrobesOnline: see SACOL2689

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  TTGCAATTTTTTAACTTTTTGCTTTTTTATCCTGTATTTATGTCTATTTACTGGATTGTC
  GGTTCAATTTATTTCTATTTTACCAGAGAAATTAGATATTCATTGAACAAGAAGCCTGAC
  ATAAATGTGGATGAATTAGAAGGCATTACATTTTTACTTGCCTGTTATAACGAAAGTGAA
  ACGATTGAAGATACGTTGTCTAATGTTCTTGCACTCAAATACGAGAAGAAAGAAATTATT
  ATCATTAATGATGGAAGTTCAGATAATACAGCAGAACTCATCTATAAAATCAAAGAAAAT
  AATGACTTTATTTTCGTCGATTTACAAGAAAACAGAGGTAAAGCCAACGCACTCAATCAA
  GGCATTAAACAGGCTTCATATGATTATGTAATGTGCTTGGATGCAGATACTATCGTTGAT
  CAAGATGCACCATATTATATGATTGAGAATTTCAAACATGATCCAAAACTTGGTGCAGTT
  ACAGGTAATCCTAGAATTCGAAATAAGAGTTCTATTTTAGGTAAAATTCAAACGATAGAA
  TATGCAAGTTTAATTGGCTGTATTAAGCGAAGTCAGACACTTGCTGGCGCAGTCAATACT
  ATTTCGGGTGTCTTCACTCTATTTAAAAAAAGTGCAGTTGTCGACGTTGGCTACTGGGAT
  ACTGATATGATTACCGAAGATATTGCAGTTTCTTGGAAATTGCATTTACGTGGATATCGT
  ATTAAGTATGAACCGCTTGCCATGTGTTGGATGTTGGTTCCAGAAACATTGGGAGGTCTT
  TGGAAGCAACGCGTGAGATGGGCTCAAGGGGGACACGAAGTATTACTACGAGACTTTTTT
  AGCACAATGAAAACGAAAAGGTTTCCTTTATATATTTTGATGTTTGAGCAAATCATCTCG
  ATTTTATGGGTATATATAGTGCTTCTATATTTAGGCTATTTGTTCATAACAGCAAACTTC
  TTAGACTATACATTTATGACATATAGTTTTTCAATATTTCTACTATCATCATTTACTATG
  ACTTTTATAAACGTTATTCAATTTACAGTCGCACTCTTTATTGATAGTCGCTACGAGAAA
  AAGAATATGGCTGGACTCATATTTGTAAGTTGGTATCCGACAGTATACTGGATTATTAAC
  GCAGCAGTAGTTCTTGTCGCATTTCCAAAAGCATTAAAACGTAAGAAAGGTGGTTACGCA
  ACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAA
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⊟Protein

⊟General

• locus tag: SACOL_RS14075 [old locus tag: SACOL2689 ]
• symbol: SACOL_RS14075
• description: poly-beta-1,6 N-acetyl-D-glucosamine synthase
• length: 412
• theoretical pI: 8.45984
• theoretical MW: 47769.2
• GRAVY: 0.112864

⊟Function

• reaction:
  EC 2.4.1.-?  ExPASy
• TIGRFAM:
  poly-beta-1,6 N-acetyl-D-glucosamine synthase (TIGR03937; EC 2.4.1.-; HMM-score: 633.2)
  and 11 more
  putative glycosyltransferase, exosortase G-associated (TIGR03111; HMM-score: 98.7)
  chitooligosaccharide synthase NodC (TIGR04242; EC 2.4.1.-; HMM-score: 85.8)
  mycofactocin system glycosyltransferase (TIGR03965; HMM-score: 57.5)
  cellulose synthase catalytic subunit (UDP-forming) (TIGR03030; EC 2.4.1.12; HMM-score: 50.2)
  Unknown function Enzymes of unknown specificity transferase 2, rSAM/selenodomain-associated (TIGR04283; EC 2.-.-.-; HMM-score: 49.7)
  glycosyltransferase, TIGR04182 family (TIGR04182; HMM-score: 32.7)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides rhamnosyltransferase (TIGR01556; EC 2.4.1.-; HMM-score: 28.8)
  hopene-associated glycosyltransferase HpnB (TIGR03469; HMM-score: 28)
  colanic acid biosynthesis glycosyltransferase WcaE (TIGR04009; EC 2.4.-.-; HMM-score: 22.6)
  hopanoid biosynthesis associated glycosyl transferase protein HpnI (TIGR03472; HMM-score: 20.4)
  colanic acid biosynthesis glycosyltransferase WcaA (TIGR04017; EC 2.4.-.-; HMM-score: 17.7)
• TheSEED: see SACOL2689
• PFAM:
  GT-A (CL0110) Glycos_transf_2; Glycosyl transferase family 2 (PF00535; HMM-score: 105.1)
  Glyco_trans_2_3; Glycosyl transferase family group 2 (PF13632; HMM-score: 93.6)
  Glyco_tranf_2_3; Glycosyltransferase like family 2 (PF13641; HMM-score: 92.3)
  and 8 more
  Glyco_transf_21; Glycosyl transferase family 21 (PF13506; HMM-score: 68.6)
  Chitin_synth_2; Chitin synthase (PF03142; HMM-score: 49.6)
  Glyco_tranf_2_2; Glycosyltransferase like family 2 (PF10111; HMM-score: 39.4)
  Glyco_tranf_2_4; Glycosyl transferase family 2 (PF13704; HMM-score: 28)
  Glyco_tranf_2_5; Glycosyltransferase like family (PF13712; HMM-score: 24.2)
  Cellulose_synt; Cellulose synthase (PF03552; HMM-score: 22.5)
  AMP_N-like (CL0356) Creatinase_N_2; Creatinase/Prolidase N-terminal domain (PF16189; HMM-score: 17.9)
  no clan defined DUF393; Protein of unknown function, DUF393 (PF04134; HMM-score: 15.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 4
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.023665
  • TAT(Tat/SPI): 0.000501
  • LIPO(Sec/SPII): 0.029446
• predicted transmembrane helices (TMHMM): 4

⊟Accession numbers

• GI: 447082174 NCBI
• RefSeq: WP_001159430 NCBI
• UniProt: see SACOL2689

⊟Protein sequence

• MQFFNFLLFYPVFMSIYWIVGSIYFYFTREIRYSLNKKPDINVDELEGITFLLACYNESETIEDTLSNVLALKYEKKEIIIINDGSSDNTAELIYKIKENNDFIFVDLQENRGKANALNQGIKQASYDYVMCLDADTIVDQDAPYYMIENFKHDPKLGAVTGNPRIRNKSSILGKIQTIEYASLIGCIKRSQTLAGAVNTISGVFTLFKKSAVVDVGYWDTDMITEDIAVSWKLHLRGYRIKYEPLAMCWMLVPETLGGLWKQRVRWAQGGHEVLLRDFFSTMKTKRFPLYILMFEQIISILWVYIVLLYLGYLFITANFLDYTFMTYSFSIFLLSSFTMTFINVIQFTVALFIDSRYEKKNMAGLIFVSWYPTVYWIINAAVVLVAFPKALKRKKGGYATWSSPDRGNTQR

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CodY, IcaR see SACOL2689

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications