PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS13705 [old locus tag: SACOL2619 ]
• pan locus tag^?: SAUPAN006300000
• symbol: SACOL_RS13705
• pan gene symbol^?: bcaP
• synonym:
• product: amino acid permease

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS13705 [old locus tag: SACOL2619 ]
• symbol: SACOL_RS13705
• product: amino acid permease
• replicon: chromosome
• strand: +
• coordinates: 2676060..2677508
• length: 1449
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (2676060..2677508) NCBI
• BioCyc: SACOL_RS13705 BioCyc
• MicrobesOnline: see SACOL2619

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  1441
  
  ATGGGAAGTTTTTTCAATAAAATAGCACGAAAAGAGGATCCGGCTATCTATCAAAATAAA
  GATGGTCATTTAAAGCGTACACTTCGGGTGCGTGATTTCTTAGCTTTAGGTGTAGGAACA
  ATTGTATCGACATCTATCTTTACGCTACCTGGCATTGTTGCTGCAGAACATGCAGGACCG
  GCCGTTGCGTTATCATTCTTACTCGCTGCTATTGTTGCTGGTTTAGTTGCATTTACTTAT
  GCAGAAATGGCTGCCGCTATGCCATTTGCAGGTTCAGCCTATTCTTGGGTCAATGTATTA
  TTTGGTGAATTTTTTGGATGGGTTGCCGGTTGGGCTCTATTAGCTGAATATTTTATCGCC
  GTAGCCTTTGTTGCATCAGGATTCTCAGCGAATTTACGCGGACTTGTGAAACCAATTGGC
  ATCGAATTACCTGCAGCATTATCAAATCCATTTGGTACAAATGGCGGTTTTATCGATATT
  ATTGCTGCTATCGTTATTTTATTAACTGCATTATTACTATCACGTGGTATGTCGGAAGCA
  GCTCGTATGGAAAATATTTTAGTTATTTTAAAAGTATTAGCTATTATTTTATTTGTCATC
  GTAGGTTTAACAGCAATAAATGTTAGTAACTATGTGCCATTTATTCCAGAACACAAAGTA
  ACTGCTACAGGTGACTTTGGTGGATGGCAAGGCATATATGCTGGTGTTTCAATGATTTTC
  TTAGCGTATATCGGTTTCGATTCTATCGCAGCAAACTCAGCAGAAGCACTTGATCCTCAA
  AAGACAATGCCTAGAGGTATTCTTGGTTCTTTAAGCGTTGCTATCGTATTATTTATTGCT
  GTAGCACTTGTGTTAGTTGGTATGTTCCATTACTCACAATACGCAAACAATGCTGAACCT
  GTTGGTTGGGCTTTACGTCAAAGTGGTCATGGTGTTGTAGCAGCTATTGTTCAAGCTATC
  TCTGTTATCGGTATGTTTACAGCATTAATTGGTATGATGTTAGCAGGCTCACGTTTACTT
  TATTCATTTGGACGTGACGGCTTATTACCTTCATGGTTAAGCCACTTAAACGACAAACAT
  TTACCTAATCGCGCACTAGTTATACTTACTATTATTGGTGTTTTAATTGGTTCTATGTTC
  CCATTCGCATTTTTAGCACAATTAATTTCAGCAGGTACACTTGTTGCATTTATGTTCGTT
  TCATTAGCAATGTATCGTTTGAGAAAGCGTGAAGGTAAAGATCTACCAATTCCTGCATTT
  AAATTACCTTTATATCCTGTGTTACCAGCAATTACATTTGTTTTAGTATTGCTAGTATTC
  TGGGGATTAGGATTCGAAGCGAAATTATACACTTTAATTTGGTTTATTGTTGGTATCATC
  CTATATTTAAGTTATGGACTTAGACATTCTAAGAAAAATGACGTAGCTGAATACCACCCA
  CCAAAATAA
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  1449
  

⊟Protein

⊟General

• locus tag: SACOL_RS13705 [old locus tag: SACOL2619 ]
• symbol: SACOL_RS13705
• description: amino acid permease
• length: 482
• theoretical pI: 9.73071
• theoretical MW: 51893.3
• GRAVY: 0.837344

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid transporter (TIGR00909; HMM-score: 325.6)
  and 13 more
  Metabolism Transport and binding proteins Amino acids, peptides and amines cationic amino acid transport permease (TIGR00906; HMM-score: 252.5)
  Metabolism Transport and binding proteins Amino acids, peptides and amines transporter, basic amino acid/polyamine antiporter (APA) family (TIGR00905; HMM-score: 114.5)
  Metabolism Transport and binding proteins Amino acids, peptides and amines ethanolamine permease (TIGR00908; HMM-score: 112.6)
  Metabolism Transport and binding proteins Amino acids, peptides and amines L-type amino acid transporter (TIGR00911; HMM-score: 90.6)
  Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid permease (yeast) (TIGR00913; HMM-score: 80.4)
  Metabolism Transport and binding proteins Amino acids, peptides and amines arginine-ornithine antiporter (TIGR03810; HMM-score: 76.5)
  Metabolism Transport and binding proteins Amino acids, peptides and amines GABA permease (TIGR01773; HMM-score: 68.4)
  permease, urea carboxylase system (TIGR03428; HMM-score: 66.4)
  Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid permease (TIGR00907; HMM-score: 50.2)
  histidine-histamine antiporter (TIGR04298; HMM-score: 42.2)
  putrescine-ornithine antiporter (TIGR04299; HMM-score: 36)
  putative glutamate/gamma-aminobutyrate antiporter (TIGR03813; HMM-score: 31)
  Metabolism Transport and binding proteins Other K-Cl cotransporter (TIGR00930; HMM-score: 2.1)
• TheSEED: see SACOL2619
• PFAM:
  APC (CL0062) AA_permease_2; Amino acid permease (PF13520; HMM-score: 193)
  and 2 more
  AA_permease; Amino acid permease (PF00324; HMM-score: 135.7)
  AA_permease_C; C-terminus of AA_permease (PF13906; HMM-score: 23)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 12
• DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.9991
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0008
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.123512
  • TAT(Tat/SPI): 0.133254
  • LIPO(Sec/SPII): 0.002933
• predicted transmembrane helices (TMHMM): 12

⊟Accession numbers

• GI: 446456571 NCBI
• RefSeq: WP_000534425 NCBI
• UniProt: see SACOL2619

⊟Protein sequence

• MGSFFNKIARKEDPAIYQNKDGHLKRTLRVRDFLALGVGTIVSTSIFTLPGIVAAEHAGPAVALSFLLAAIVAGLVAFTYAEMAAAMPFAGSAYSWVNVLFGEFFGWVAGWALLAEYFIAVAFVASGFSANLRGLVKPIGIELPAALSNPFGTNGGFIDIIAAIVILLTALLLSRGMSEAARMENILVILKVLAIILFVIVGLTAINVSNYVPFIPEHKVTATGDFGGWQGIYAGVSMIFLAYIGFDSIAANSAEALDPQKTMPRGILGSLSVAIVLFIAVALVLVGMFHYSQYANNAEPVGWALRQSGHGVVAAIVQAISVIGMFTALIGMMLAGSRLLYSFGRDGLLPSWLSHLNDKHLPNRALVILTIIGVLIGSMFPFAFLAQLISAGTLVAFMFVSLAMYRLRKREGKDLPIPAFKLPLYPVLPAITFVLVLLVFWGLGFEAKLYTLIWFIVGIILYLSYGLRHSKKNDVAEYHPPK

⊟Experimental data

• experimentally validated: see SACOL2619
• protein localization: see SACOL2619
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CodY see SACOL2619

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications