PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS13235 [old locus tag: SACOL2527 ]
• pan locus tag^?: SAUPAN006159000
• symbol: SACOL_RS13235
• pan gene symbol^?: fbp
• synonym:
• product: fructose 1,6-bisphosphatase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS13235 [old locus tag: SACOL2527 ]
• symbol: SACOL_RS13235
• product: fructose 1,6-bisphosphatase
• replicon: chromosome
• strand: +
• coordinates: 2587978..2589942
• length: 1965
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (2587978..2589942) NCBI
• BioCyc: SACOL_RS13235 BioCyc
• MicrobesOnline: see SACOL2527

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  1561
  1621
  1681
  1741
  1801
  1861
  1921
  
  ATGACTCAAATTACTGAAAAAGAATTAAAAAAGAAGTATTTAGATTTACTATCCCAAAAT
  TTTGATACTCCAGAAAAACTTGCAACTGAAATTATCAATTTAGAATCAATTTTAGAATTA
  CCTAAAGGTACGGAACATTTCGTCAGCGATTTACATGGTGAATATGAAGCTTTCCAACAC
  GTATTACGCAACGGTTCTGGGAACGTGCGAGCGAAAATCAATGATATTTTCAAAGAGAGA
  CTTTCAACTAAGGAGCTTAATGACTTAACTGCTCTTGTCTACTATCCAGAAGACAAATTA
  AAATTGATTAAAAGTGATTTCCAAAATTGCGGTCAACTTAATGTCTGGTATATCACAACA
  ATCGAACATTTAATTGAGTTAATTAAATATTGTTCTTCAAAATATACGCGTTCAAAACTG
  CGTAAAGCATTGCCAAAACAATACGTTTATATTATTGAGGAACTACTGTATAAAAGTAAT
  GAATATCAAAATAAAAAATCATATTACGAAACACTTGTTAACCAAGTAATTGAACTTAAA
  CAGGCAGATGATTTAATTATTGGACTCGCTTATTCCGTACAACGCTTAGTCGTCGATCAT
  TTACACGTTGTCGGTGATATTTATGATCGTGGACCACAACCAGATAAAATTATGGATACA
  CTGATTAATTATCATTCCCTAGATATTCAATGGGGTAATCATGATGTGCTTTGGGTTGGA
  GCCTATGCTGGGTCAAAAGTATGCTTAGCAAACTTACTTCGAATTTGTGCACGTTATGAT
  AATTTAGATATTATCGAAGACGCTTATGGCATTAATTTAAGACCACTGCTTACTTTAGCT
  GAAAAATACTATGACGCAGATAATCCTGCTTTTAAGCCTAAAAAAAGACCTGACAAACAC
  GAACGTTTAACTCAACGTGAAGAAAGTCAAATTACTAAAATTCATCAAGCTATTGCGATG
  ATTCAATTCAAGTTAGAAATACCAATTATTAAACGTCGTCCAAATTTCGAAATGGAAGAA
  CGTCTTGTGCTTGAAAAGGTTAATTATGATACAAATGAAATTACAGTTTATGGTAATACA
  TACCCATTGAAAGACACATGTTTCCAAACTGTCAATCGTGATAATCCAGCAGAATTACTA
  CCTGAAGAAGAAGAAGTCATGAATAAACTATTATTGTCATTCCAACAATCTGAAAAATTA
  CGTCGTCATATGTCTTTCTTGATGCGTAAAGGCTCTCTTTACTTACCATATAATGGCAAT
  TTACTCATTCATGGTTGTATTCCAGTTGATGAAAATGGTGAGATGGAATCATTTGAAATT
  GATGGTCATACTTACAGCGGCCAAGAATTATTAGATGTGTTTGAGTATCATGTCCGTAAA
  TCATTTGATGAAAAAGAAAATACTGATGACTTATCGACGGATTTAGTTTGGTATTTATGG
  ACTGGGAAATATTCGTCACTATTTGGTAAACGTGCCATGACTACGTTTGAGCGATACTTT
  ATTGCAGATAAAGCTTCTCATAAAGAAGAAAAGAATCCGTACTATCATCTTCGTGAAGAT
  GTGAATATGGTTCGTAAAATGCTCAGTGATTTCGGATTAAATCCAGATGAAGGACGCATT
  ATTAATGGTCACACACCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGA
  AAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCA
  GGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAAC
  GCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGAT
  AAAGAATTACAACGTAAGAAGATTCGTGATACCAATATTGGTAAAGATCTTCAAGCTCAA
  ATAGATATTTTGAAAATGTTAATGCATGATCGTTACTTAGACTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1500
  1560
  1620
  1680
  1740
  1800
  1860
  1920
  1965
  

⊟Protein

⊟General

• locus tag: SACOL_RS13235 [old locus tag: SACOL2527 ]
• symbol: SACOL_RS13235
• description: fructose 1,6-bisphosphatase
• length: 654
• theoretical pI: 6.04366
• theoretical MW: 76174.5
• GRAVY: -0.520336

⊟Function

• reaction:
  EC 3.1.3.11?  ExPASy
  Fructose-bisphosphatase D-fructose 1,6-bisphosphate + H₂O = D-fructose 6-phosphate + phosphate
• TIGRFAM:
• TheSEED: see SACOL2527
• PFAM:
  Calcineurin (CL0163) FBPase_2; Firmicute fructose-1,6-bisphosphatase (PF06874; HMM-score: 940.9)
  and 1 more
  no clan defined GP24_25; Tail assembly protein Gp24 and Gp25 (PF17388; HMM-score: 14.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Mn²⁺
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002427
  • TAT(Tat/SPI): 0.000086
  • LIPO(Sec/SPII): 0.000178
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446114313 NCBI
• RefSeq: WP_000192168 NCBI
• UniProt: see SACOL2527

⊟Protein sequence

• MTQITEKELKKKYLDLLSQNFDTPEKLATEIINLESILELPKGTEHFVSDLHGEYEAFQHVLRNGSGNVRAKINDIFKERLSTKELNDLTALVYYPEDKLKLIKSDFQNCGQLNVWYITTIEHLIELIKYCSSKYTRSKLRKALPKQYVYIIEELLYKSNEYQNKKSYYETLVNQVIELKQADDLIIGLAYSVQRLVVDHLHVVGDIYDRGPQPDKIMDTLINYHSLDIQWGNHDVLWVGAYAGSKVCLANLLRICARYDNLDIIEDAYGINLRPLLTLAEKYYDADNPAFKPKKRPDKHERLTQREESQITKIHQAIAMIQFKLEIPIIKRRPNFEMEERLVLEKVNYDTNEITVYGNTYPLKDTCFQTVNRDNPAELLPEEEEVMNKLLLSFQQSEKLRRHMSFLMRKGSLYLPYNGNLLIHGCIPVDENGEMESFEIDGHTYSGQELLDVFEYHVRKSFDEKENTDDLSTDLVWYLWTGKYSSLFGKRAMTTFERYFIADKASHKEEKNPYYHLREDVNMVRKMLSDFGLNPDEGRIINGHTPVKEINGEDPIKADGKMLVIDGGFSKAYQSTTGIAGYTLLYNSFGMQLVAHQQFNAKEKILSEGIDELSIKRVVDKELQRKKIRDTNIGKDLQAQIDILKMLMHDRYLD

⊟Experimental data

• experimentally validated: see SACOL2527
• protein localization: see SACOL2527
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CcpA see SACOL2527

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications