PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS09115 [old locus tag: SACOL1781 ]
• pan locus tag^?: SAUPAN004393000
• symbol: SACOL_RS09115
• pan gene symbol^?: isdH
• synonym:
• product: iron-regulated surface determinant protein H

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS09115 [old locus tag: SACOL1781 ]
• symbol: SACOL_RS09115
• product: iron-regulated surface determinant protein H
• replicon: chromosome
• strand: -
• coordinates: 1821378..1824065
• length: 2688
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (1821378..1824065) NCBI
• BioCyc: SACOL_RS09115 BioCyc
• MicrobesOnline: see SACOL1781

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  481
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  841
  901
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  1021
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  ATGAACAAACATCACCCAAAATTAAGGTCTTTCTATTCTATTAGAAAATCAACTCTAGGC
  GTTGCATCGGTCATTGTCAGTACACTATTTTTAATTACTTCTCAACATCAAGCACAAGCA
  GCAGAAAATACAAATACTTCAGATAAAATCTCGGAAAATCAAAATAATAATGCAACTACA
  ACTCAGCCACCTAAGGATACAAATCAAACACAACCTGCTACGCAACCAGCAAACACTGCG
  AAAAACTATCCTGCAGCGGATGAATCACTTAAAGATGCAATTAAAGATCCTGCATTAGAA
  AATAAAGAACATGATATAGGTCCAAGAGAACAAGTCAATTTCCAGTTATTAGATAAAAAC
  AATGAAACGCAGTACTATCACTTTTTCAGCATCAAAGATCCAGCAGATGTGTATTACACT
  AAAAAGAAAGCAGAAGTTGAATTAGACATCAATACTGCTTCAACATGGAAGAAGTTTGAA
  GTCTATGAAAACAATCAAAAATTGCCAGTGAGACTTGTATCATATAGTCCTGTACCAGAA
  GACCATGCCTATATTCGATTCCCAGTTTCAGATGGCACACAAGAATTGAAAATTGTTTCT
  TCGACTCAAATTGATGATGGAGAAGAAACAAATTATGATTATACTAAATTAGTATTTGCT
  AAACCTATTTATAACGATCCTTCACTTGTAAAATCAGATACAAATGATGCAGTAGTAACG
  AATGATCAATCAAGTTCAGTCGCAAGTAATCAAACAAACACGAATACATCTAATCAAAAT
  ACATCAACGATCAACAATGCTAATAATCAACCGCAGGCAACGACCAATATGAGTCAACCT
  GCACAACCAAAATCGTCAACGAATGCAGATCAAGCGTCAAGCCAACCAGCTCATGAAACA
  AATTCTAATGGTAATACTAACGATAAAACGAATGAGTCAAGTAATCAGTCGGATGTTAAT
  CAACAGTATCCACCAGCAGATGAATCACTACAAGATGCAATTAAAAACCCGGCTATCATC
  GATAAAGAACATACAGCTGATAATTGGCGACCAATTGATTTTCAAATGAAAAATGATAAA
  GGTGAAAGACAGTTCTATCATTATGCTAGTACTGTTGAACCAGCAACTGTCATTTTTACA
  AAAACAGGACCAATAATTGAATTAGGTTTAAAGACAGCTTCAACATGGAAGAAATTTGAA
  GTTTATGAAGGTGACAAAAAGTTACCAGTCGAATTAGTATCATATGATTCTGATAAAGAT
  TATGCCTATATTCGTTTCCCAGTATCTAATGGTACGAGAGAAGTTAAAATTGTGTCATCT
  ATTGAATATGGTGAGAACATCCATGAAGACTATGATTATACGCTAATGGTCTTTGCACAG
  CCTATTACTAATAACCCAGACGACTATGTGGATGAAGAAACATACAATTTACAAAAATTA
  TTAGCTCCGTATCACAAAGCTAAAACGTTAGAAAGACAAGTTTATGAATTAGAAAAATTA
  CAAGAGAAATTGCCAGAAAAATATAAGGCGGAATATAAAAAGAAATTAGATCAAACTAGA
  GTAGAGTTAGCTGATCAAGTTAAATCAGCAGTGACGGAATTTGAAAATGTTACACCTACA
  AATGATCAATTAACAGATTTACAAGAAGCGCATTTTGTTGTTTTTGAAAGTGAAGAAAAT
  AGTGAGTCAGTTATGGACGGCTTTGTTGAACATCCATTCTATACAGCAACTTTAAATGGT
  CAAAAATATGTAGTGATGAAAACAAAGGATGACAGTTACTGGAAAGATTTAATTGTAGAA
  GGTAAACGTGTCACTACTGTTTCTAAAGATCCTAAAAATAATTCTAGAACGCTGATTTTC
  CCATATATACCTGACAAAGCAGTTTACAATGCGATTGTTAAAGTCGTTGTGGCAAACATT
  GGTTATGAAGGTCAATATCATGTCAGAATTATAAATCAGGATATCAATACAAAAGATGAT
  GATACATCACAAAATAACACGAGTGAACCGCTAAATGTACAAACAGGACAAGAAGGTAAG
  GTTGCTGATACAGATGTAGCTGAAAATAGCAGCACTGCAACAAATCCTAAAGATGCGTCT
  GATAAAGCAGATGTGATAGAACCAGAGTCTGACGTGGTTAAAGATGCTGATAATAATATT
  GATAAAGATGTGCAACATGATGTTGATCATTTATCCGATATGTCGGATAATAATCACTTC
  GATAAATATGATTTAAAAGAAATGGATACTCAAATTGCCAAAGATACTGATAGAAATGTG
  GATAAAGATGCCGATAATAGCGTTGGTATGTCATCTAATGTCGATACTGATAAAGACTCT
  AATAAAAATAAAGACAAAGTCATACAGCTGAATCATATTGCCGATAAAAATAATCATACT
  GGAAAAGCAGCAAAGCTTGACGTAGTGAAACAAAATTATAATAATACAGACAAAGTTACT
  GACAAAAAAACAACTGAACATCTGCCGAGTGATATTCATAAAACTGTAGATAAAACAGTG
  AAAACAAAAGAAAAAGCCGGCACACCATCGAAAGAAAACAAACTTAGTCAATCTAAAATG
  CTACCAAAAACTGGAGAAACAACTTCAAGCCAATCATGGTGGGGCTTATATGCGTTATTA
  GGTATGTTAGCTTTATTCATTCCTAAATTCAGAAAAGAATCTAAATAA
  60
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  2688
  

⊟Protein

• 4IJ2
  PDB
• 4XS0
  PDB

Protein Data Bank: 4IJ2

Protein Data Bank: 4XS0

⊟General

• locus tag: SACOL_RS09115 [old locus tag: SACOL1781 ]
• symbol: SACOL_RS09115
• description: iron-regulated surface determinant protein H
• length: 895
• theoretical pI: 4.84648
• theoretical MW: 100934
• GRAVY: -0.943911

⊟Function

• TIGRFAM:
  haptoglobin-binding heme uptake protein HarA (TIGR03658; HMM-score: 2116.9)
  and 4 more
  heme uptake protein IsdB (TIGR03657; HMM-score: 667)
  gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 38)
  heme uptake protein IsdC (TIGR03656; HMM-score: 26.4)
  Cell structure Cell envelope Other LPXTG cell wall anchor domain (TIGR01167; HMM-score: 23.8)
• TheSEED: see SACOL1781
• PFAM:
  E-set (CL0159) NEAT; Iron Transport-associated domain (PF05031; HMM-score: 134.4)
  no clan defined Isd_H_B_linker; Iron-regulated surface determinant protein H/B, linker domain (PF20861; HMM-score: 129.5)
  and 3 more
  YSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 29.2)
  DUF2283; Protein of unknown function (DUF2283) (PF10049; HMM-score: 22.2)
  DUF4404; Domain of unknown function (DUF4404) (PF14357; HMM-score: 7.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cellwall
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 10
  • Extracellular Score: 0
  • Internal Helices: 0
• DeepLocPro: Cell wall & surface
  • Cytoplasmic Score: 0.0012
  • Cytoplasmic Membrane Score: 0.0268
  • Cell wall & surface Score: 0.8947
  • Extracellular Score: 0.0773
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 40 aa
  • SP(Sec/SPI): 0.990402
  • TAT(Tat/SPI): 0.008094
  • LIPO(Sec/SPII): 0.000828
  • Cleavage Site: CS pos: 40-41. AQA-AE. Pr: 0.9895
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446955517 NCBI
• RefSeq: WP_001032773 NCBI
• UniProt: see SACOL1781

⊟Protein sequence

• MNKHHPKLRSFYSIRKSTLGVASVIVSTLFLITSQHQAQAAENTNTSDKISENQNNNATTTQPPKDTNQTQPATQPANTAKNYPAADESLKDAIKDPALENKEHDIGPREQVNFQLLDKNNETQYYHFFSIKDPADVYYTKKKAEVELDINTASTWKKFEVYENNQKLPVRLVSYSPVPEDHAYIRFPVSDGTQELKIVSSTQIDDGEETNYDYTKLVFAKPIYNDPSLVKSDTNDAVVTNDQSSSVASNQTNTNTSNQNTSTINNANNQPQATTNMSQPAQPKSSTNADQASSQPAHETNSNGNTNDKTNESSNQSDVNQQYPPADESLQDAIKNPAIIDKEHTADNWRPIDFQMKNDKGERQFYHYASTVEPATVIFTKTGPIIELGLKTASTWKKFEVYEGDKKLPVELVSYDSDKDYAYIRFPVSNGTREVKIVSSIEYGENIHEDYDYTLMVFAQPITNNPDDYVDEETYNLQKLLAPYHKAKTLERQVYELEKLQEKLPEKYKAEYKKKLDQTRVELADQVKSAVTEFENVTPTNDQLTDLQEAHFVVFESEENSESVMDGFVEHPFYTATLNGQKYVVMKTKDDSYWKDLIVEGKRVTTVSKDPKNNSRTLIFPYIPDKAVYNAIVKVVVANIGYEGQYHVRIINQDINTKDDDTSQNNTSEPLNVQTGQEGKVADTDVAENSSTATNPKDASDKADVIEPESDVVKDADNNIDKDVQHDVDHLSDMSDNNHFDKYDLKEMDTQIAKDTDRNVDKDADNSVGMSSNVDTDKDSNKNKDKVIQLNHIADKNNHTGKAAKLDVVKQNYNNTDKVTDKKTTEHLPSDIHKTVDKTVKTKEKAGTPSKENKLSQSKMLPKTGETTSSQSWWGLYALLGMLALFIPKFRKESK

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: Fur* see SACOL1781

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications