PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS08990 [old locus tag: SACOL1758 ]
• pan locus tag^?: SAUPAN004361000
• symbol: SACOL_RS08990
• pan gene symbol^?: ald2
• synonym:
• product: alanine dehydrogenase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS08990 [old locus tag: SACOL1758 ]
• symbol: SACOL_RS08990
• product: alanine dehydrogenase
• replicon: chromosome
• strand: -
• coordinates: 1797492..1798610
• length: 1119
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (1797492..1798610) NCBI
• BioCyc: SACOL_RS08990 BioCyc
• MicrobesOnline: see SACOL1758

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
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  301
  361
  421
  481
  541
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  661
  721
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  841
  901
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  1021
  1081
  
  ATGAAAATTGGTATACCAAGGGAGATTAAAAATAATGAAAATCGTGTTGGTTTATCACCA
  AGTGGTGTGCACGCTTTAGTAGAAAGTGGGCATACTGTTTTAGTGGAAACAAATGCGGGT
  TCAGGATCATTCTTTGAAGATGTAGATTACAAAGAAGCAGGTGCTGAGATTGTTGCTGAA
  CAAGCAAAAGTTTGGGATGTGGATATGGTTATTAAAGTTAAAGAACCACTTGAATCTGAA
  TATCCATATTTTAAAGAAGGGCTTGTATTATTCACTTATCTTCATTTAGCAAATGAAGAA
  AAATTAACACAAGCTTTGATAGATAGAAAAGTAATTAGTATTGCATATGAGACTGTGCAG
  TTACCAGACCGATCTTTACCATTGTTATCACCAATGAGTGAGGTAGCAGGAAGAATGTCA
  GCTCAAGTTGGCGCAGAGTTCCTACAAAAACTTAATGGTGGTATGGGAATTCTACTAGGT
  GGTGTCCCAGGAGTACCTAAGGGTAAAGTAACTATTATCGGTGGTGGTCAAGCAGGAACA
  AATGCAGCTAAAATTGCACTAGGACTAGGTGCAGATGTTACGATTTTAGATGTTAATCCA
  AAGCGTTTACAACAATTAGATGATTTATTCGGTGGACGTGTACATACAATTATGTCAAAT
  CCGTTGAATATTGAGTTGTATGTTAAACAAAGTGATTTAGTAATAGGTGCAGTTTTAATT
  CCAGGTGCTAAAGCGCCAAGACTTGTAACAGAAGACATGATTAAACAAATGAAAAATGGG
  TCAGTTATTATTGACATTGCTATTGATCAAGGCGGTATTTTTGAAACAACTGATAAAATT
  ACGACACATGATGATCCTACATATATTAAGCATGGTGTGGTTCATTATGCAGTTGCAAAT
  ATGCCAGGTGCAGTACCGCGTACTTCGACGTTAGCTTTAAATAATGCTACGCTACCTTAT
  GCGCTCATGCTAGCTAATAAAGGGTATAGAGAAGCATTTAAATCAAATCAACCATTATCA
  TTAGGTTTAAATACTTACAAAGGTCACGTAACCAATAAAGGCGTTGCAGAGGCATTTGAA
  ATGGAATATAAATCTGTAGAAGAAGCATTACAATTATAA
  60
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  1020
  1080
  1119
  

⊟Protein

⊟General

• locus tag: SACOL_RS08990 [old locus tag: SACOL1758 ]
• symbol: SACOL_RS08990
• description: alanine dehydrogenase
• length: 372
• theoretical pI: 5.6562
• theoretical MW: 40104.9
• GRAVY: -0.0215054

⊟Function

• reaction:
  EC 1.4.1.1?  ExPASy
  Alanine dehydrogenase L-alanine + H₂O + NAD⁺ = pyruvate + NH₃ + NADH
• TIGRFAM:
  Metabolism Energy metabolism Amino acids and amines alanine dehydrogenase (TIGR00518; EC 1.4.1.1; HMM-score: 575.8)
  and 15 more
  Metabolism Energy metabolism Electron transport NAD(P)(+) transhydrogenase (AB-specific), alpha subunit (TIGR00561; EC 1.6.1.2; HMM-score: 151.3)
  Cellular processes Cellular processes Sporulation and germination dipicolinic acid synthetase, A subunit (TIGR02853; HMM-score: 26.8)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin glutamyl-tRNA reductase (TIGR01035; EC 1.2.1.70; HMM-score: 19.3)
  dihydrolipoyl dehydrogenase (TIGR01350; EC 1.8.1.4; HMM-score: 18.6)
  mycofactocin system FadH/OYE family oxidoreductase 2 (TIGR03997; EC 1.-.-.-; HMM-score: 16)
  Metabolism Energy metabolism Electron transport glutathione-disulfide reductase (TIGR01424; EC 1.8.1.7; HMM-score: 15.8)
  Metabolism Energy metabolism Amino acids and amines adenosylhomocysteinase (TIGR00936; EC 3.3.1.1; HMM-score: 13.8)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylaminoimidazole carboxylase, ATPase subunit (TIGR01161; EC 4.1.1.21; HMM-score: 13.3)
  6-hydroxycyclohex-1-ene-1-carbonyl-CoA dehydrogenase (TIGR03201; EC 1.1.1.-; HMM-score: 13.3)
  nucleotide sugar dehydrogenase (TIGR03026; HMM-score: 12.6)
  Unknown function Enzymes of unknown specificity flavoprotein, HI0933 family (TIGR00275; HMM-score: 12.5)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll geranylgeranyl reductase family (TIGR02032; EC 1.3.1.-; HMM-score: 12.4)
  mycothione reductase (TIGR03452; EC 1.8.1.15; HMM-score: 12.3)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides surface carbohydrate biosynthesis protein (TIGR04396; HMM-score: 11.5)
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA (TIGR00136; HMM-score: 11.3)
• TheSEED: see SACOL1758
• PFAM:
  NADP_Rossmann (CL0063) AlaDh_PNT_C; Alanine dehydrogenase/PNT, C-terminal domain (PF01262; HMM-score: 284.6)
  and 15 more
  Form_Glyc_dh (CL0325) AlaDh_PNT_N; Alanine dehydrogenase/PNT, N-terminal domain (PF05222; HMM-score: 155.2)
  NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 30.3)
  Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 26.5)
  Shikimate_DH; Shikimate / quinate 5-dehydrogenase (PF01488; HMM-score: 26.5)
  Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 21.1)
  3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 18.5)
  F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 18.3)
  NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 17.1)
  GIDA; Glucose inhibited division protein A (PF01134; HMM-score: 16.9)
  TrkA_N; TrkA-N domain (PF02254; HMM-score: 16.8)
  THF_DHG_CYH_C; Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain (PF02882; HMM-score: 16.6)
  NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 14.1)
  HI0933_like; HI0933-like protein (PF03486; HMM-score: 13.7)
  AdoHcyase_NAD; S-adenosyl-L-homocysteine hydrolase, NAD binding domain (PF00670; HMM-score: 13.1)
  UDPG_MGDP_dh_N; UDP-glucose/GDP-mannose dehydrogenase family, NAD binding domain (PF03721; HMM-score: 12.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.0094
  • TAT(Tat/SPI): 0.001248
  • LIPO(Sec/SPII): 0.000687
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446612652 NCBI
• RefSeq: WP_000689998 NCBI
• UniProt: see SACOL1758

⊟Protein sequence

• MKIGIPREIKNNENRVGLSPSGVHALVESGHTVLVETNAGSGSFFEDVDYKEAGAEIVAEQAKVWDVDMVIKVKEPLESEYPYFKEGLVLFTYLHLANEEKLTQALIDRKVISIAYETVQLPDRSLPLLSPMSEVAGRMSAQVGAEFLQKLNGGMGILLGGVPGVPKGKVTIIGGGQAGTNAAKIALGLGADVTILDVNPKRLQQLDDLFGGRVHTIMSNPLNIELYVKQSDLVIGAVLIPGAKAPRLVTEDMIKQMKNGSVIIDIAIDQGGIFETTDKITTHDDPTYIKHGVVHYAVANMPGAVPRTSTLALNNATLPYALMLANKGYREAFKSNQPLSLGLNTYKGHVTNKGVAEAFEMEYKSVEEALQL

⊟Experimental data

• experimentally validated: see SACOL1758
• protein localization: see SACOL1758
• quantitative data / protein copy number per cell: see SACOL1758
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CcpA see SACOL1758

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications