PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS08875 [old locus tag: SACOL1736 ]
• pan locus tag^?: SAUPAN004312000
• symbol: SACOL_RS08875
• pan gene symbol^?: mutM
• synonym:
• product: DNA-formamidopyrimidine glycosylase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS08875 [old locus tag: SACOL1736 ]
• symbol: SACOL_RS08875
• product: DNA-formamidopyrimidine glycosylase
• replicon: chromosome
• strand: -
• coordinates: 1766901..1767773
• length: 873
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (1766901..1767773) NCBI
• BioCyc: SACOL_RS08875 BioCyc
• MicrobesOnline: see SACOL1736

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGCCCGAATTACCAGAAGTAGAACATGTAAAAAGAGGAATTGAACCCTACGTTATAAAT
  CAAAAAATTGAACACGTTATATTTTCGGATAAAGTAATCGAAGGTAAAGCTCAAGGCAAG
  GAAACAATTATAAAAGGAATTGAATTAGATACTTTTAAAACTTTATCGGAAGGCTATACC
  ATTACAAATGTAGAACGAAGAAGTAAATATATCGTTTTTCAGCTAGATAATAAACGAGAA
  CAACGTACATTAATTAGTCATTTAGGTATGGCGGGCGGTTTTTTCATTGTAGATGAACTT
  GAAGATATTATGATACCTAATTATCGTAAGCATTGGCATGTTATTTTTGAGTTGTCAAAT
  GACAAAAAGTTAATTTATTCAGATATACGTCGTTTTGGAGAAATTAGAAATGTTGCGTCT
  GTAGCATCTTATCCTTCATTTTTAGAAATAGCGCCTGAACCTTTTTCAAATGAAGCATTG
  ACGTATTATTTAAATCGAATTCATCAACAGTCTAATAAGAATAAACCAATCAAACAAGTG
  ATTTTGGATCATAAAGTAATCGCAGGTTGTGGAAATATTTACGCATGTGAAGCGTTGTTT
  CGTGCAGGTGTTTTACCAGATAAAAAAGTAAAAGACTTAACACACCAACAACAAGAAATG
  GTTTTTTATTATGTTAGAGAAGTACTAGAAGAAGGTATTAAATATGGCGGTACAAGTATT
  TCAGATTATCGTCATGCTGATGGAAAAACTGGTGAGATGCAATTGCATCTTAATGTATAT
  AAGCAGCCGGTATGCAAAGTGTGTGGAAGTCAAATTGAAACAAAAATCATCGCGACAAGA
  AATAGTCATTATTGTCCAGTATGTCAAAAATAA
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⊟Protein

⊟General

• locus tag: SACOL_RS08875 [old locus tag: SACOL1736 ]
• symbol: SACOL_RS08875
• description: DNA-formamidopyrimidine glycosylase
• length: 290
• theoretical pI: 8.12881
• theoretical MW: 33411.2
• GRAVY: -0.39069

⊟Function

• reaction:
  EC 3.2.2.23?  ExPASy
  DNA-formamidopyrimidine glycosylase Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine
  EC 4.2.99.18?  ExPASy
  DNA-(apurinic or apyrimidinic site) lyase The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA-formamidopyrimidine glycosylase (TIGR00577; EC 3.2.2.23; HMM-score: 235.5)
  and 1 more
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein uS13 (TIGR03631; HMM-score: 15.1)
• TheSEED: see SACOL1736
• PFAM:
  no clan defined Fapy_DNA_glyco; Formamidopyrimidine-DNA glycosylase N-terminal domain (PF01149; HMM-score: 102.7)
  and 5 more
  H2TH (CL0303) H2TH; Formamidopyrimidine-DNA glycosylase H2TH domain (PF06831; HMM-score: 74.5)
  Tbcl_zf (CL0839) zf-FPG_IleRS; Zinc finger found in FPG and IleRS (PF06827; HMM-score: 24.4)
  HTH (CL0123) HTH_66; Putative DNA-binding HTH domain (PF21321; HMM-score: 17.7)
  H2TH (CL0303) NFACT_N; NFACT N-terminal and middle domains (PF05833; HMM-score: 16)
  Zn_Beta_Ribbon (CL0167) DZR_2; Double zinc ribbon domain (PF18912; HMM-score: 12.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Zn²⁺
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9946
  • Cytoplasmic Membrane Score: 0.0004
  • Cell wall & surface Score: 0.0004
  • Extracellular Score: 0.0047
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005618
  • TAT(Tat/SPI): 0.000165
  • LIPO(Sec/SPII): 0.000521
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 447037196 NCBI
• RefSeq: WP_001114452 NCBI
• UniProt: see SACOL1736

⊟Protein sequence

• MPELPEVEHVKRGIEPYVINQKIEHVIFSDKVIEGKAQGKETIIKGIELDTFKTLSEGYTITNVERRSKYIVFQLDNKREQRTLISHLGMAGGFFIVDELEDIMIPNYRKHWHVIFELSNDKKLIYSDIRRFGEIRNVASVASYPSFLEIAPEPFSNEALTYYLNRIHQQSNKNKPIKQVILDHKVIAGCGNIYACEALFRAGVLPDKKVKDLTHQQQEMVFYYVREVLEEGIKYGGTSISDYRHADGKTGEMQLHLNVYKQPVCKVCGSQIETKIIATRNSHYCPVCQK

⊟Experimental data

• experimentally validated: see SACOL1736
• protein localization: see SACOL1736
• quantitative data / protein copy number per cell: see SACOL1736
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications