From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS08765 [old locus tag: SACOL1716 ]
  • pan locus tag?: SAUPAN004284000
  • symbol: SACOL_RS08765
  • pan gene symbol?: hemD
  • synonym:
  • product: uroporphyrinogen III methyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS08765 [old locus tag: SACOL1716 ]
  • symbol: SACOL_RS08765
  • product: uroporphyrinogen III methyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 1747032..1747700
  • length: 669
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (1747032..1747700) NCBI
  • BioCyc: SACOL_RS08765 BioCyc
  • MicrobesOnline: see SACOL1716

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAAGCCAGTTGTAGTTATGACACAAACAAATGACATGCAAAGTGATTTAGTATCAATT
    ATTCACAAACCCTTTATTGATATAAAGCCACTAAATTTTGATATTCATTTGTTAAATCAA
    CGCTATGACTGGCTTATTTTTTCATCTAAAAATGCTGTGAAATTCTTTTATAAATATCTA
    AAAGGAATTAATGTTGATAACATTGCTGTGATAGGAAGTAAGACAGCGCAATATTGTGAA
    TCACTTGGCATTCGAGTTGATTTTATGCCAAACGACTTTTCTCAAGAAGGATTTTTAAAA
    TCATTTAATCAAACTAACCAAAAAATACTTTTGCCTTCGAGTGAATTGGCGAGACCATTG
    TTATTAGCAGCGTTATCTAAAGATAATGAAGTTGTTAAAATAGATTTATATACTTCAGTG
    CCTAACAAACAAAATATACAAGATGTTAAAGAAATGATAGAACATCAACAAATCGATGCA
    TTAACATTTTCAAGTTCGTCGGCAGTACGTTATTATTTTAATGAAGGATTTGTACCAAAA
    TTCAAGTCGTATTTTGCTATTGGAGAACAAACAGCACGGACCATTAAATCATATCAACAA
    CCAGTAACAATTGCAGAAATTCAAACACTCGAATCACTAATTGAAAAGATTTTAGAAAGT
    AGGGGCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    669

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS08765 [old locus tag: SACOL1716 ]
  • symbol: SACOL_RS08765
  • description: uroporphyrinogen III methyltransferase
  • length: 222
  • theoretical pI: 7.58445
  • theoretical MW: 25418.1
  • GRAVY: -0.167117

⊟Function[edit | edit source]

  • reaction:
    EC 4.2.1.75?  ExPASy
    Uroporphyrinogen-III synthase Hydroxymethylbilane = uroporphyrinogen III + H2O
  • TIGRFAM:
  • TheSEED: see SACOL1716
  • PFAM:
    no clan defined HEM4; Uroporphyrinogen-III synthase HemD (PF02602; HMM-score: 136.6)
    and 1 more
    HHH (CL0198) IMS_HHH; IMS family HHH motif (PF11798; HMM-score: 13.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9481
    • Cytoplasmic Membrane Score: 0.0493
    • Cell wall & surface Score: 0.0007
    • Extracellular Score: 0.0019
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001459
    • TAT(Tat/SPI): 0.000066
    • LIPO(Sec/SPII): 0.000586
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKPVVVMTQTNDMQSDLVSIIHKPFIDIKPLNFDIHLLNQRYDWLIFSSKNAVKFFYKYLKGINVDNIAVIGSKTAQYCESLGIRVDFMPNDFSQEGFLKSFNQTNQKILLPSSELARPLLLAALSKDNEVVKIDLYTSVPNKQNIQDVKEMIEHQQIDALTFSSSSAVRYYFNEGFVPKFKSYFAIGEQTARTIKSYQQPVTIAEIQTLESLIEKILESRG

⊟Experimental data[edit | edit source]

  • experimentally validated: see SACOL1716
  • protein localization: see SACOL1716
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • data available for JSNZ

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SACOL_RS08765&oldid=101457"