PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS08595 [old locus tag: SACOL1685 ]
• pan locus tag^?: SAUPAN004235000
• symbol: SACOL_RS08595
• pan gene symbol^?: aspS
• synonym:
• product: aspartate--tRNA ligase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS08595 [old locus tag: SACOL1685 ]
• symbol: SACOL_RS08595
• product: aspartate--tRNA ligase
• replicon: chromosome
• strand: -
• coordinates: 1713268..1715034
• length: 1767
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (1713268..1715034) NCBI
• BioCyc: SACOL_RS08595 BioCyc
• MicrobesOnline: see SACOL1685

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAGTAAGAGAACAACTTATTGTGGATTAGTTACTGAGGCATTTTTAGGACAAGAAATT
  ACATTAAAAGGATGGGTTAACAATCGTCGTGACCTTGGTGGATTGATTTTCGTTGATTTA
  AGAGATAGAGAAGGAATTGTACAAGTCGTGTTTAATCCTGCATTTTCAGAAGAGGCATTG
  AAAATTGCTGAAACAGTACGTTCTGAATATGTTGTAGAAGTTCAAGGTACAGTTACGAAG
  CGTGACCCTGAAACAGTTAATCCTAAAATTAAAACTGGCCAAGTTGAAGTACAAGTTACA
  AATATTAAAGTGATTAATAAATCTGAGACACCACCATTTTCTATAAATGAAGAAAATGTT
  AACGTTGATGAAAATATTCGATTAAAATACCGTTATTTAGATTTACGTCGTCAAGAGTTA
  GCGCAAACATTTAAAATGAGACATCAAATTACACGTTCTATTCGTCAATATTTGGATGAT
  GAAGGGTTCTTTGACATCGAAACACCAGTACTAACGAAGTCAACACCTGAGGGTGCACGT
  GACTATTTAGTACCATCTCGTGTTCATGATGGTGAATTTTATGCATTACCACAATCACCA
  CAATTATTTAAGCAATTATTGATGATTAGTGGATTTGACAAATACTACCAAATCGTAAAA
  TGCTTCCGTGACGAAGATTTACGTGCAGATCGTCAACCTGAATTTACACAAGTCGATATT
  GAAATGAGTTTTGTAGACCAAGAAGATGTGATGCAAATGGGTGAAGAAATGCTTAAAAAA
  GTTGTTAAAGAAGTTAAAGGCGTTGAAATTAATGGCGCTTTCCCACGCATGACATATAAA
  GAAGCGATGCGTCGCTATGGTTCTGATAAACCAGATACACGTTTTGAAATGGAATTAATT
  GACGTTTCTCAATTAGGACGTGATATGGACTTTAAAGTATTTAAAGATACTGTTGAAAAT
  GATGGTGAAATTAAAGCAATTGTCGCTAAAGGTGCAGCTGAACAATATACTCGTAAAGAT
  ATGGATGCTTTAACAGAATTTGTAAACATCTATGGTGCTAAAGGATTAGCGTGGGTTAAA
  GTTGTGGAAGATGGTTTGACAGGTCCAATTGGACGTTTCTTTGAAACAGAAAATGTTGAA
  ACATTACTTACATTAACTGGTGCTGAAGCTGGTGACTTAGTAATGTTTGTTGCAGACAAA
  CCAAATGTCGTTGCACAAAGTTTAGGTGCATTACGTGTCAAATTAGCTAAAGAATTAGGT
  TTAATCGATGAAACAAAATTAAACTTCTTATGGGTGACAGATTGGCCATTATTAGAATAT
  GATGAAGATGCGAAACGTTACGTTGCAGCACATCATCCATTTACATCTCCAAAAGAAGCT
  GATATTGCTAAGCTTGGCACTGCGCCAGAAGAAGCTGAGGCAAATGCTTATGACATAGTA
  TTAAATGGTTATGAATTAGGTGGCGGTTCAATCAGAATTCATGATGGTGAGTTACAAGAA
  AAAATGTTCGAAGTTCTTGGATTTACTAAAGAACAAGCACAAGAACAGTTCGGCTTCTTA
  CTAGATGCATTTAAATATGGTGCACCACCACATGGCGGTATTGCATTAGGATTAGACCGA
  TTAGTCATGTTATTGACTAATAGAACAAACTTGAGAGATACAATCGCATTCCCTAAAACA
  GCATCTGCTACATGTTTATTAACGAATGCGCCTGGTGAAGTTTCTGATAAACAATTAGAA
  GAACTTTCTTTGCGAATTCGTCACTAA
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  1767
  

⊟Protein

⊟General

• locus tag: SACOL_RS08595 [old locus tag: SACOL1685 ]
• symbol: SACOL_RS08595
• description: aspartate--tRNA ligase
• length: 588
• theoretical pI: 4.69429
• theoretical MW: 66598.4
• GRAVY: -0.355272

⊟Function

• reaction:
  EC 6.1.1.12?  ExPASy
  Aspartate--tRNA ligase ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp)
• TIGRFAM:
  Genetic information processing Protein synthesis tRNA aminoacylation aspartate--tRNA ligase (TIGR00459; EC 6.1.1.-; HMM-score: 855.8)
  and 7 more
  Genetic information processing Protein synthesis tRNA aminoacylation aspartate--tRNA(Asn) ligase (TIGR00458; EC 6.1.1.23; HMM-score: 253.8)
  Genetic information processing Protein synthesis tRNA aminoacylation asparagine--tRNA ligase (TIGR00457; EC 6.1.1.22; HMM-score: 167.8)
  Genetic information processing Protein synthesis tRNA aminoacylation lysine--tRNA ligase (TIGR00499; EC 6.1.1.6; HMM-score: 166.9)
  Unknown function General EF-P lysine aminoacylase GenX (TIGR00462; HMM-score: 91.8)
  Genetic information processing Protein synthesis tRNA aminoacylation histidine--tRNA ligase (TIGR00442; EC 6.1.1.21; HMM-score: 24.4)
  Genetic information processing Protein synthesis tRNA aminoacylation pyrrolysine--tRNA ligase, C-terminal region (TIGR02367; EC 6.1.1.26; HMM-score: 16.2)
  Genetic information processing Protein synthesis tRNA aminoacylation phenylalanine--tRNA ligase, alpha subunit (TIGR00468; EC 6.1.1.20; HMM-score: 9.9)
• TheSEED: see SACOL1685
• PFAM:
  tRNA_synt_II (CL0040) tRNA-synt_2; tRNA synthetases class II (D, K and N) (PF00152; HMM-score: 379.3)
  and 4 more
  GAD (CL0250) GAD; GAD domain (PF02938; HMM-score: 99.3)
  OB (CL0021) tRNA_anti-codon; OB-fold nucleic acid binding domain (PF01336; HMM-score: 65.7)
  tRNA_synt_II (CL0040) tRNA-synt_2d; tRNA synthetases class II core domain (F) (PF01409; HMM-score: 20.6)
  tRNA-synt_2b; tRNA synthetase class II core domain (G, H, P, S and T) (PF00587; HMM-score: 16.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 10
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004914
  • TAT(Tat/SPI): 0.001285
  • LIPO(Sec/SPII): 0.00119
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 445966944 NCBI
• RefSeq: WP_000044799 NCBI
• UniProt: see SACOL1685

⊟Protein sequence

• MSKRTTYCGLVTEAFLGQEITLKGWVNNRRDLGGLIFVDLRDREGIVQVVFNPAFSEEALKIAETVRSEYVVEVQGTVTKRDPETVNPKIKTGQVEVQVTNIKVINKSETPPFSINEENVNVDENIRLKYRYLDLRRQELAQTFKMRHQITRSIRQYLDDEGFFDIETPVLTKSTPEGARDYLVPSRVHDGEFYALPQSPQLFKQLLMISGFDKYYQIVKCFRDEDLRADRQPEFTQVDIEMSFVDQEDVMQMGEEMLKKVVKEVKGVEINGAFPRMTYKEAMRRYGSDKPDTRFEMELIDVSQLGRDMDFKVFKDTVENDGEIKAIVAKGAAEQYTRKDMDALTEFVNIYGAKGLAWVKVVEDGLTGPIGRFFETENVETLLTLTGAEAGDLVMFVADKPNVVAQSLGALRVKLAKELGLIDETKLNFLWVTDWPLLEYDEDAKRYVAAHHPFTSPKEADIAKLGTAPEEAEANAYDIVLNGYELGGGSIRIHDGELQEKMFEVLGFTKEQAQEQFGFLLDAFKYGAPPHGGIALGLDRLVMLLTNRTNLRDTIAFPKTASATCLLTNAPGEVSDKQLEELSLRIRH

⊟Experimental data

• experimentally validated: see SACOL1685
• protein localization: see SACOL1685
• quantitative data / protein copy number per cell: see SACOL1685
• interaction partners:

  SACOL_RS03055		DNA-directed RNA polymerase subunit beta'   (data from MRSA252)
  SACOL_RS03075		elongation factor G   (data from MRSA252)
  SACOL_RS03080		elongation factor Tu   (data from MRSA252)
  SACOL_RS05635		pyruvate dehydrogenase E1 component subunit beta   (data from MRSA252)
  SACOL_RS05645		dihydrolipoyl dehydrogenase   (data from MRSA252)
  SACOL_RS06450		succinyl-CoA ligase subunit alpha   (data from MRSA252)
  SACOL_RS06505		30S ribosomal protein S2   (data from MRSA252)
  SACOL_RS08905		isocitrate dehydrogenase (NADP(+))   (data from MRSA252)
  SACOL_RS08930		pyruvate kinase   (data from MRSA252)
  SACOL_RS08995		universal stress protein UspA   (data from MRSA252)
  SACOL_RS11230		glutamine--fructose-6-phosphate aminotransferase   (data from MRSA252)
  SACOL_RS11435		Asp23/Gls24 family envelope stress response protein   (data from MRSA252)
  SACOL_RS11615		30S ribosomal protein S9   (data from MRSA252)
  SACOL_RS11695		30S ribosomal protein S5   (data from MRSA252)
  SACOL_RS11750		30S ribosomal protein S3   (data from MRSA252)

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: T-box(Asn) see SACOL1685

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: see SACOL1685

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications