PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS05240 [old locus tag: SACOL1028 ]
• pan locus tag^?: SAUPAN003203000
• symbol: SACOL_RS05240
• pan gene symbol^?: htrA2
• synonym:
• product: serine protease

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS05240 [old locus tag: SACOL1028 ]
• symbol: SACOL_RS05240
• product: serine protease
• replicon: chromosome
• strand: +
• coordinates: 1036704..1039028
• length: 2325
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (1036704..1039028) NCBI
• BioCyc: SACOL_RS05240 BioCyc
• MicrobesOnline: see SACOL1028

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  1561
  1621
  1681
  1741
  1801
  1861
  1921
  1981
  2041
  2101
  2161
  2221
  2281
  
  ATGGAGTGGACATTAGTGGATATTGGTAAAAAACATGTAATTCCTAAAAGTCAGTACCGA
  CGTAAGCGTCGTGAATTCTTCCACAACGAAGACAGAGAAGAAAATTTAAATCAACATCAA
  GATAAACAAAATATAGATAATACAACATCAAAAAAAGCAGATAAGCAAATACATAAAGAT
  TCAATTGATAAGCACGAACGTTTTAAAAATAGTTTATCATCGCATTTAGAACAGAGAAAC
  CGTGATGTTAATGAGAATAAAGCTGAAGAAAGTAAAAGTAATCAGGATAGTAAGTCAGCA
  TATAACAGAGATCATTATTTAACAGACGATGTATCTAAAAAACAAAATTCATTAGATTCA
  GTGGACCAAGATACAGAGAAATCAAAATATTATGAGCAAAATTCTGAAGCGACTTTATCA
  ACTAAATCAACCGATAAAGTAGAATCAACTGAAATGAGAAAGCTAAGTTCAGATAAAAAC
  AAAGTTGGTCATGAAGAGCAACATGTACTTTCTAAACCTTCAGAACATGATAAAGAGACT
  AGAATTGATTCTGAGTCTTCAAGAACTGATTCAGACAGCTCGATGCAGACAGAGAAAATA
  AAAAAAGACAGTTCAGATGGAAATAAAAGTAGTAATCTGAAATCTGAAGTAATATCAGAC
  AAATCAAATACAGTACCAAAATTGTCGGAATCTGATGATGAAGTAAATAATCAGAAGCCA
  TTAACTTTACCGGAAGAACAGAAATTGAAAAGACAGCAAAGTCAAAATGAGCAAACAAAA
  ACCTATACATATGGTGATAGCGAACAAAATGACAAGTCTAATCATGAAAATGATTTAAGT
  CATCATATACCATCGATAAGTGATGATAAAGATAACGTCATGAGAGAAAATCATATTGTT
  GACGATAATCCTGATAATGATATCAATACACCATCATTATCAAAAACAGATGACGATCGA
  AAACTTGATGAAAAAATTCATGTTGAAGATAAACATAAACAAAATGCAGACTCGTCTGAA
  ACGGTGGGATATCAAAGTCAGTCAACTGCATCTCATCGTAGCACTGAAAAAAGAAATATT
  TCTATTAATGACCATGATAAATTAAACGGTCAAAAAACAAATACAAAGACATCGGCAAAT
  AATAATCAAAAAAAGGCTACATCAAAATTGAACAAAGGGCGCGCTACGAATAATAATTAT
  AGTGACATTTTGAAAAAGTTTTGGATGATGTATTGGCCTAAATTAGTTATTCTAATGGGT
  ATTATTATTCTAATTGTTATTTTGAATGCCATTTTTAATAATGTGAACAAAAATGATCGC
  ATGAATGATAATAATGATGCAGATGCTCAAAAATATACGACAACGATGAAAAATGCCAAT
  AACACAGTTAAATCGGTCGTTACAGTTGAAAATGAAACATCAAAAGATTCATCATTACCT
  AAAGATAAAGCATCTCAAGACGAAGTGGGATCAGGTGTTGTATATAAAAAATCTGGAGAT
  ACGTTATATATTGTTACGAATGCACACGTTGTCGGTGATAAAGAAAATCAAAAAATAACT
  TTCTCGAATAATAAAAGTGTTGTTGGGAAAGTGCTTGGTAAAGATAAATGGTCAGATTTA
  GCTGTTGTTAAAGCAACTTCTTCAGACAGTTCAGTGAAAGAGATAGCTATTGGAGATTCA
  AATAATTTAGTGTTAGGAGAGCCAATATTAGTCGTAGGTAATCCACTTGGTGTAGACTTT
  AAAGGCACTGTGACAGAAGGTATTATTTCAGGTCTGAACAGAAATGTTCCTATTGATTTC
  GATAAAGATAATAAATATGATATGTTGATGAAAGCTTTCCAAATTGATGCATCAGTAAAT
  CCAGGTAACTCGGGTGGTGCTGTCGTCAATAGAGAAGGAAAATTAATAGGTGTAGTTGCA
  GCTAAAATTAGTATGCCAAACGTTGAAAATATGTCATTTGCAATACCTGTTAATGAAGTA
  CAAAAGATTGTAAAAGATTTAGAAACAAAAGGTAAAATTGACTATCCCGATGTAGGTGTT
  AAAATGAAGAATATTGCCAGTCTAAATAGTTTTGAAAGACAAGCAGTTAAATTGCCAGGA
  AAAGTTAAGAACGGTGTTGTTGTAGATCAAGTTGACAACAATGGTTTAGCAGATCAATCT
  GGTCTGAAAAAAGGTGATGTAATTACTGAATTAGATGGCAAACTTTTAGAAGATGATTTA
  CGCTTTAGGCAGATTATATTTAGTCATAAAGATGACTTGAAATCAATTACAGCGAAGATT
  TATAGAGATGGTAAAGAGAAAGAAATTAATATTAAACTAAAATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1500
  1560
  1620
  1680
  1740
  1800
  1860
  1920
  1980
  2040
  2100
  2160
  2220
  2280
  2325
  

⊟Protein

⊟General

• locus tag: SACOL_RS05240 [old locus tag: SACOL1028 ]
• symbol: SACOL_RS05240
• description: serine protease
• length: 774
• theoretical pI: 6.88081
• theoretical MW: 87059.9
• GRAVY: -1.02868

⊟Function

• reaction:
  EC 3.4.21.-?  ExPASy
• TIGRFAM:
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 219.6)
  Genetic information processing Protein fate Protein folding and stabilization peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 219.6)
  and 3 more
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides periplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 139.4)
  Signal transduction Regulatory functions Protein interactions periplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 139.4)
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides RIP metalloprotease RseP (TIGR00054; EC 3.4.24.-; HMM-score: 17.9)
• TheSEED: see SACOL1028
• PFAM:
  Peptidase_PA (CL0124) Trypsin_2; Trypsin-like peptidase domain (PF13365; HMM-score: 111.1)
  and 6 more
  Trypsin; Trypsin (PF00089; HMM-score: 52)
  PDZ-like (CL0466) PDZ_2; PDZ domain (PF13180; HMM-score: 46.1)
  PDZ_6; PDZ domain (PF17820; HMM-score: 24.7)
  PDZ; PDZ domain (PF00595; HMM-score: 23.6)
  no clan defined DUF4834; Domain of unknown function (DUF4834) (PF16118; HMM-score: 15.9)
  Peptidase_PA (CL0124) Peptidase_S32; Equine arteritis virus serine endopeptidase S32 (PF05579; HMM-score: 14)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helix: 1
• DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.9277
  • Cell wall & surface Score: 0.0016
  • Extracellular Score: 0.0706
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005064
  • TAT(Tat/SPI): 0.00043
  • LIPO(Sec/SPII): 0.001643
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: see SACOL1028
• RefSeq: WP_000455278 NCBI
• UniProt: see SACOL1028

⊟Protein sequence

• MEWTLVDIGKKHVIPKSQYRRKRREFFHNEDREENLNQHQDKQNIDNTTSKKADKQIHKDSIDKHERFKNSLSSHLEQRNRDVNENKAEESKSNQDSKSAYNRDHYLTDDVSKKQNSLDSVDQDTEKSKYYEQNSEATLSTKSTDKVESTEMRKLSSDKNKVGHEEQHVLSKPSEHDKETRIDSESSRTDSDSSMQTEKIKKDSSDGNKSSNLKSEVISDKSNTVPKLSESDDEVNNQKPLTLPEEQKLKRQQSQNEQTKTYTYGDSEQNDKSNHENDLSHHIPSISDDKDNVMRENHIVDDNPDNDINTPSLSKTDDDRKLDEKIHVEDKHKQNADSSETVGYQSQSTASHRSTEKRNISINDHDKLNGQKTNTKTSANNNQKKATSKLNKGRATNNNYSDILKKFWMMYWPKLVILMGIIILIVILNAIFNNVNKNDRMNDNNDADAQKYTTTMKNANNTVKSVVTVENETSKDSSLPKDKASQDEVGSGVVYKKSGDTLYIVTNAHVVGDKENQKITFSNNKSVVGKVLGKDKWSDLAVVKATSSDSSVKEIAIGDSNNLVLGEPILVVGNPLGVDFKGTVTEGIISGLNRNVPIDFDKDNKYDMLMKAFQIDASVNPGNSGGAVVNREGKLIGVVAAKISMPNVENMSFAIPVNEVQKIVKDLETKGKIDYPDVGVKMKNIASLNSFERQAVKLPGKVKNGVVVDQVDNNGLADQSGLKKGDVITELDGKLLEDDLRFRQIIFSHKDDLKSITAKIYRDGKEKEINIKLK

⊟Experimental data

• experimentally validated: see SACOL1028
• protein localization: see SACOL1028
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications