PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

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⊟Summary

• organism: Staphylococcus aureus COL
locus tag: SACOL_RS04995 [old locus tag: SACOL0976 ]
pan locus tag^?: SAUPAN003108000
symbol: SACOL_RS04995
pan gene symbol^?: —
• synonym:
• product: HAD family phosphatase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS04995 [old locus tag: SACOL0976 ]
• symbol: SACOL_RS04995
• product: HAD family phosphatase
• replicon: chromosome
• strand: -
• coordinates: 982437..983261
• length: 825
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (982437..983261) NCBI
• BioCyc: SACOL_RS04995 BioCyc
• MicrobesOnline: see SACOL0976

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGCAACCACATTTAATATGTCTAGACTTAGACGGAACATTATTAAACGATAACAAAGAA
  ATTTCATCATATACTAAACAAGTATTAAATGAATTACAACAACGTGGACACCAAATTATG
  ATTGCGACTGGCAGACCTTATCGTGCAAGTCAAATGTATTATCATGAATTAAATTTAACG
  ACACCAATTGTTAATTTTAATGGCGCTTACGTACATCACCCTAAAGATAAAAACTTCAAA
  ACTTGCCATGAAATTTTAGATTTAGGCATCGCACAAAACATTATTCAAGGATTACAACAA
  TATCAAGTATCGAATATTATAGCAGAAGTGAAAGATTATGTTTTCATTAACAATCATGAT
  CCAAGATTATTTGAAGGTTTTTCAATGGGTAATCCAAGAATTCAAACTGGTAATTTACTT
  GTCCACTTGAAAGAATCCCCTACCTCAATTTTAATTGAAGCCGAAGAAAGTAAAATACCT
  GAAATCAAAAATATGCTTACTCATTTTTATGCCGATCATATTGAGCATCGACGCTGGGGC
  GCACCATTCCCTGTCATTGAAATTGTAAAACTTGGTATTAATAAAGCAAGAGGCATTGAG
  CAAGTTAGACAATTTTTAAATATTGACCGAAATAATATTATTGCATTCGGTGATGAAGAT
  AATGATATTGAAATGATTGAGTACGCGCGTCACGGTGTTGCTATGGAAAATGGTTTGCAA
  GAACTTAAAGATGTAGCGAACAATATTACATTCAACAATAATGAAGATGGCATTGGTCGA
  TATTTGAATGATTTCTTTAATTTAAATATTAGATATTACTGTTAA
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⊟Protein

⊟General

• locus tag: SACOL_RS04995 [old locus tag: SACOL0976 ]
• symbol: SACOL_RS04995
• description: HAD family phosphatase
• length: 274
• theoretical pI: 5.90828
• theoretical MW: 31668.7
• GRAVY: -0.419708

⊟Function

• ⊞TIGRFAM:
  Unknown function Enzymes of unknown specificity Cof-like hydrolase (TIGR00099; HMM-score: 205.2)
  and 16 more
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IIB (TIGR01484; HMM-score: 93.3)
  phosphoglycolate phosphatase, TA0175-type (TIGR01487; EC 3.1.3.18; HMM-score: 78)
  sucrose-phosphate phosphatase subfamily (TIGR01482; EC 3.1.3.-; HMM-score: 64.9)
  mannosyl-3-phosphoglycerate phosphatase family (TIGR01486; EC 3.1.3.-; HMM-score: 51)
  mannosyl-3-phosphoglycerate phosphatase (TIGR02461; EC 3.1.3.70; HMM-score: 43.9)
  Unknown function General mannosyl-3-phosphoglycerate phosphatase homolog (TIGR02463; EC 3.1.3.-; HMM-score: 38)
  sucrose-phosphate synthase, sucrose phosphatase-like domain (TIGR02471; HMM-score: 31.3)
  Metabolism Amino acid biosynthesis Serine family phosphoserine phosphatase SerB (TIGR00338; EC 3.1.3.3; HMM-score: 29.1)
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IIIA (TIGR01662; HMM-score: 27.1)
  sucrose phosphatase (TIGR01485; EC 3.1.3.24; HMM-score: 26.8)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, YrbI family (TIGR01670; EC 3.1.3.45; HMM-score: 24)
  Unknown function Enzymes of unknown specificity HAD phosphoserine phosphatase-like hydrolase, family IB (TIGR01488; HMM-score: 23.9)
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IB (TIGR01490; HMM-score: 19.6)
  HAD phosphatase, family IIIC (TIGR01681; HMM-score: 16.6)
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IIA (TIGR01460; HMM-score: 16.3)
  phosphoserine phosphatase-like hydrolase, archaeal (TIGR01491; HMM-score: 14.5)
• TheSEED: see SACOL0976
• ⊞PFAM:
  HAD (CL0137) Hydrolase_3; haloacid dehalogenase-like hydrolase (PF08282; HMM-score: 221.9)
  and 9 more
  S6PP; Sucrose-6F-phosphate phosphohydrolase (PF05116; HMM-score: 49.3)
  Hydrolase; haloacid dehalogenase-like hydrolase (PF00702; HMM-score: 39.8)
  HAD_2; haloacid dehalogenase-like hydrolase (PF13419; HMM-score: 28.6)
  HAD; haloacid dehalogenase-like hydrolase (PF12710; HMM-score: 19.2)
  Trehalose_PPase; Trehalose-phosphatase (PF02358; HMM-score: 18.1)
  Hydrolase_6; haloacid dehalogenase-like hydrolase (PF13344; HMM-score: 17.7)
  Acid_PPase; Acid Phosphatase (PF12689; HMM-score: 17.1)
  DUF2608; Protein of unknown function (DUF2608) (PF11019; HMM-score: 13.1)
  LNS2_PITM1-3; PITM 1-3, LNS2 domain (PF24694; HMM-score: 12.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9978
  • Cytoplasmic Membrane Score: 0.0013
  • Cell wall & surface Score: 0.0001
  • Extracellular Score: 0.0008
• LocateP:
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00715
  • TAT(Tat/SPI): 0.000497
  • LIPO(Sec/SPII): 0.001067
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 447105180 NCBI
• RefSeq: WP_001182436 NCBI
• UniProt: see SACOL0976

⊟Protein sequence

• MQPHLICLDLDGTLLNDNKEISSYTKQVLNELQQRGHQIMIATGRPYRASQMYYHELNLTTPIVNFNGAYVHHPKDKNFKTCHEILDLGIAQNIIQGLQQYQVSNIIAEVKDYVFINNHDPRLFEGFSMGNPRIQTGNLLVHLKESPTSILIEAEESKIPEIKNMLTHFYADHIEHRRWGAPFPVIEIVKLGINKARGIEQVRQFLNIDRNNIIAFGDEDNDIEMIEYARHGVAMENGLQELKDVANNITFNNNEDGIGRYLNDFFNLNIRYYC

⊟Experimental data

• experimentally validated: see SACOL0976
• protein localization: see SACOL0976
• quantitative data / protein copy number per cell: see SACOL0976
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: see SACOL0976

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications