PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS04395 [old locus tag: SACOL0856 ]
• pan locus tag^?: SAUPAN002779000
• symbol: SACOL_RS04395
• pan gene symbol^?: clfA
• synonym:
• product: clumping factor A

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS04395 [old locus tag: SACOL0856 ]
• symbol: SACOL_RS04395
• product: clumping factor A
• replicon: chromosome
• strand: +
• coordinates: 880643..883444
• length: 2802
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (880643..883444) NCBI
• BioCyc: SACOL_RS04395 BioCyc
• MicrobesOnline: see SACOL0856

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  121
  181
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  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  1561
  1621
  1681
  1741
  1801
  1861
  1921
  1981
  2041
  2101
  2161
  2221
  2281
  2341
  2401
  2461
  2521
  2581
  2641
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  2761
  
  ATGAATATGAAGAAAAAAGAAAAACACGCAATTCGGAAAAAATCGATTGGCGTGGCTTCA
  GTGCTTGTAGGTACGTTAATCGGTTTTGGACTACTCAGCAGTAAAGAAGCAGATGCAAGT
  GAAAATAGTGTTACGCAATCTGATAGCGCAAGTAACGAAAGCAAAAGTAATGATTCAAGT
  AGCGTTAGTGCTGCACCTAAAACAGACGACACAAACGTGAGTGATACTAAAACATCGTCA
  AACACTAATAATGGCGAAACGAGTGTGGCGCAAAATCCAGCACAACAGGAAACGACACAA
  TCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACG
  ACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATGCGGAGGAA
  TTAGTGAATCAAACAAGTAATGAAACGACTTCTAATGATACTAATACAGTATCATCTGTA
  AATTCACCTCAAAATTCTACAAATGCGGAAAATGTTTCAACAACGCAAGATACTTCAACT
  GAAGCAACACCTTCAAACAATGAATCAGCTCCACAGAGTACAGATGCAAGTAATAAAGAT
  GTAGTTAATCAAGCGGTTAATACAAGTGCGCCTAGAATGAGAGCATTTAGTTTAGCGGCA
  GTAGCTGCAGATGCACCGGCAGCTGGCACAGATATTACGAATCAGTTGACGAATGTGACA
  GTTGGTATTGACTCTGGTACGACTGTGTATCCGCACCAAGCAGGTTATGTCAAACTGAAT
  TATGGTTTTTCAGTGCCTAATTCTGCTGTTAAAGGTGACACATTCAAAATAACTGTACCT
  AAAGAATTAAACTTAAATGGTGTAACTTCAACTGCTAAAGTGCCACCAATTATGGCTGGA
  GATCAAGTATTGGCAAATGGTGTAATCGATAGTGATGGTAATGTTATTTATACATTTACA
  GACTATGTAAATACTAAAGATGATGTAAAAGCAACTTTGACCATGCCCGCTTATATTGAC
  CCTGAAAATGTTAAAAAGACAGGTAATGTGACATTGGCTACTGGCATAGGTAGTACAACA
  GCAAACAAAACAGTATTAGTAGATTATGAAAAATATGGTAAGTTTTATAACTTATCTATT
  AAAGGTACAATTGACCAAATCGATAAAACAAATAATACGTATCGTCAGACAATTTATGTC
  AATCCAAGTGGAGATAACGTTATTGCGCCGGTTTTAACAGGTAATTTAAAACCAAATACG
  GATAGTAATGCATTAATAGATCAGCAAAATACAAGTATTAAAGTATATAAAGTAGATAAT
  GCAGCTGATTTATCTGAAAGTTACTTTGTGAATCCAGAAAACTTTGAGGATGTCACTAAT
  AGTGTGAATATTACATTCCCAAATCCAAATCAATATAAAGTAGAGTTTAATACGCCTGAT
  GATCAAATTACAACACCGTATATAGTAGTTGTTAATGGTCATATTGATCCGAATAGCAAA
  GGTGATTTAGCTTTACGTTCAACTTTATATGGGTATAACTCGAATATAATTTGGCGCTCT
  ATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGAT
  AAACCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGAT
  TCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGAT
  TCGGGTAGTGATTCTACATCAGATAGTGGTTCAGATTCAGCGAGTGATTCAGATTCAGCA
  AGTGATTCAGACTCAGCGAGTGATTCAGATTCAGCAAGCGATTCCGACTCAGCGAGCGAT
  TCCGACTCAGACAATGACTCGGATTCAGATAGCGATTCTGACTCAGACAGTGACTCAGAT
  TCCGACAGTGACTCAGATTCAGATAGCGATTCTGACTCAGACAGTGACTCAGATTCAGAT
  AGCGATTCAGATTCAGATAGCGATTCAGATTCCGACAGTGATTCCGACTCAGACAGCGAT
  TCTGACTCCGACAGTGATTCCGACTCAGACAGCGATTCAGATTCCGACAGTGATTCCGAC
  TCAGATAGCGATTCCGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGATTCAGAC
  AGCGATTCAGATTCAGATAGCGATTCAGATTCCGACAGTGACTCAGATTCCGACAGTGAC
  TCGGATTCAGATAGCGATTCAGATTCCGACAGTGACTCAGATTCCGACAGTGACTCAGAC
  TCAGACAGTGATTCGGATTCAGCGAGTGATTCGGATTCAGATAGTGATTCCGACTCCGAC
  AGTGACTCGGATTCAGATAGCGACTCAGACTCGGATAGCGACTCGGATTCAGATAGCGAT
  TCGGACTCAGATAGCGATTCAGAATCAGACAGCGATTCAGAATCAGACAGCGATTCAGAT
  TCAGACAGCGACTCAGACAGTGACTCAGATTCAGATAGTGACTCGGATTCAGCGAGTGAT
  TCAGACTCAGGTAGTGACTCCGATTCATCAAGTGATTCCGACTCAGAAAGTGATTCAAAT
  AGCGATTCCGAGTCAGGTTCTAACAATAATGTAGTTCCGCCTAATTCACCTAAAAATGGT
  ACTAATGCTTCTAATAAAAATGAGGCTAAAGATAGTAAAGAACCATTACCAGATACAGGT
  TCTGAAGATGAAGCAAATACGTCACTAATTTGGGGATTATTAGCATCAATAGGTTCATTA
  CTACTTTTCAGAAGAAAAAAAGAAAATAAAGATAAGAAATAA
  60
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  2760
  2802
  

⊟Protein

⊟General

• locus tag: SACOL_RS04395 [old locus tag: SACOL0856 ]
• symbol: SACOL_RS04395
• description: clumping factor A
• length: 933
• theoretical pI: 3.44162
• theoretical MW: 96997.2
• GRAVY: -1.0865

⊟Function

• TIGRFAM:
  gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 28.4)
  Cell structure Cell envelope Other LPXTG cell wall anchor domain (TIGR01167; HMM-score: 27.7)
• TheSEED: see SACOL0856
• PFAM:
  Adhesin (CL0204) SdrG_C_C; C-terminus of bacterial fibrinogen-binding adhesin (PF10425; HMM-score: 165.2)
  and 3 more
  E-set (CL0159) Big_8; Bacterial Ig domain (PF17961; HMM-score: 90.6)
  no clan defined YSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 36.6)
  Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 23.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cellwall
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 10
  • Extracellular Score: 0
  • Internal Helices: 0
• DeepLocPro: Cell wall & surface
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.0023
  • Cell wall & surface Score: 0.8895
  • Extracellular Score: 0.1082
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 39 aa
  • SP(Sec/SPI): 0.955997
  • TAT(Tat/SPI): 0.010304
  • LIPO(Sec/SPII): 0.029148
  • Cleavage Site: CS pos: 39-40. ADA-SE. Pr: 0.8951
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 446978967 NCBI
• RefSeq: WP_001056223 NCBI
• UniProt: see SACOL0856

⊟Protein sequence

• MNMKKKEKHAIRKKSIGVASVLVGTLIGFGLLSSKEADASENSVTQSDSASNESKSNDSSSVSAAPKTDDTNVSDTKTSSNTNNGETSVAQNPAQQETTQSSSTNATTEETPVTGEATTTTTNQANTPATTQSSNTNAEELVNQTSNETTSNDTNTVSSVNSPQNSTNAENVSTTQDTSTEATPSNNESAPQSTDASNKDVVNQAVNTSAPRMRAFSLAAVAADAPAAGTDITNQLTNVTVGIDSGTTVYPHQAGYVKLNYGFSVPNSAVKGDTFKITVPKELNLNGVTSTAKVPPIMAGDQVLANGVIDSDGNVIYTFTDYVNTKDDVKATLTMPAYIDPENVKKTGNVTLATGIGSTTANKTVLVDYEKYGKFYNLSIKGTIDQIDKTNNTYRQTIYVNPSGDNVIAPVLTGNLKPNTDSNALIDQQNTSIKVYKVDNAADLSESYFVNPENFEDVTNSVNITFPNPNQYKVEFNTPDDQITTPYIVVVNGHIDPNSKGDLALRSTLYGYNSNIIWRSMSWDNEVAFNNGSGSGDGIDKPVVPEQPDEPGEIEPIPEDSDSDPGSDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSASDSDSDNDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSESDSDSESDSDSDSDSDSDSDSDSDSDSDSASDSDSGSDSDSSSDSDSESDSNSDSESGSNNNVVPPNSPKNGTNASNKNEAKDSKEPLPDTGSEDEANTSLIWGLLASIGSLLLFRRKKENKDKK

⊟Experimental data

• experimentally validated: see SACOL0856
• protein localization: see SACOL0856
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: SigB* see SACOL0856

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications