PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS03700 [old locus tag: SACOL0720 ]
• pan locus tag^?: SAUPAN002541000
• symbol: SACOL_RS03700
• pan gene symbol^?: vraG
• synonym:
• product: ABC transporter permease

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS03700 [old locus tag: SACOL0720 ]
• symbol: SACOL_RS03700
• product: ABC transporter permease
• replicon: chromosome
• strand: +
• coordinates: 743090..744979
• length: 1890
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (743090..744979) NCBI
• BioCyc: SACOL_RS03700 BioCyc
• MicrobesOnline: see SACOL0720

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGACCTTTAACGAGATAATATTTAAAAATTTCCGTCAAAATTTATCACATTATGCCATC
  TATCTTTTTTCGTTAATTACGAGTGTAGTATTGTATTTTAGCTTTGTAGCATTAAAATAC
  GCTCATAAACTAAACATGACAGAGTCATATCCAATTATAAAGGAAGGCTCACAAGTCGGA
  AGCTACTTTCTATTTTTCATCATAATTGCATTTTTGTTATATGCCAATGTGTTATTTATT
  AAACGACGAAGTTATGAGCTTGCATTATATCAAACATTAGGTTTATCTAAATTCAACATT
  ATTTATATACTAATGCTCGAACAATTACTAATATTTATAATTACGGCAATATTAGGTATT
  ATTATTGGTATTTTTGGTTCGAAACTGTTATTAATGATTGTCTTTACATTATTAGGAATT
  AAAGAAAAGGTTCCAATTATTTTTAGTTTGAGGGCGGTATTTGAAACATTAATGTTAATC
  GGTGTCGCTTATTTTTTAACATCTGCTCAAAATTTTATATTAGTGTTCAAACAATCTATT
  TCACAGATGTCAAAGAATAACCAGGTTAAAGAAACAAATCATAATAAAATTACATTTGAA
  GAGGTTGTTTTAGGCATCTTAGGTATAGTATTGATTACCACAGGATACTATCTATCTTTG
  AACATTGTTCAATATTATGATTCTATCGGTACACTTATGTTTATTTTATTGTCAACTGTG
  ATTGGGGCATACTTATTTTTTAAAAGCTCTGTTTCTCTAGTTTTTAAAATGGTGAAGAAG
  TTTAGAAAAGGTGTTATAAGTGTAAATGATGTCATGTTCTCATCATCTATTATGTATCGT
  ATTAAGAAAAATGCTTTTTCACTTACGGTCATGGCAATCATTTCAGCGATTACTGTTTCA
  GTTCTTTGCTTTGCTGCTATAAGTAGAGCGTCCTTATCAAGTGAAATAAAATATACTGCA
  CCACACGACGTTACAATTAAAGACCAACAAAAAGCTAATCAATTAGCAAGTGAATTAAAC
  AATCAAAAAATTCCTCATTTTTATAATTATAAAGAAGTAATTCATACGAAATTGTATAAA
  GATAATTTATTTGATGTAAAAGCGAAAGAACCATACAATGTAACAATTACTAGTGATAAA
  TACATCCCTAATACTGATTTGAAACGTGGGCAAGCTGATTTATTTGTAGCGGAAGGTTCT
  ATCAAAGATTTAGTGAAACATAAGAAGCATGGTAAGGCAATTATAGGAACGAAAAAACAT
  CATGTTAATATTAAGTTACGTAAAGATATTAATAAAATCTATTTTATGACAGATGTTGAT
  TTAGGTGGACCAACGTTTGTCTTAAATGACAAAGACTATCAAGAAATAAGAAAGTATACA
  AAGGCAAAGCATATCGTCTCTCAATTTGGATTCGATTTGAAACATAAAAAAGATGCTTTA
  GCATTAGAAAAAGCGAAAAATAAAGTTGATAAATCTATTGAAACAAGAAGTGAAGCGATA
  AGCTCAATATCAAGTTTAACCGGAATATTATTATTTGTAACATCATTTTTAGGTATTACA
  TTCTTGATTGCTGTATGTTGCATTATATACATAAAGCAAATAGATGAAACCGAAGATGAG
  TTAGAGAATTATAGTATTTTGAGAAAGCTTGGATTTACACAAAAAGATATGGCAAGGGGA
  CTAAAGTTTAAAATTATGTTTAATTTTGGGTTACCTTTAGTTATTGCACTATCACATGCA
  TATTTTACATCATTAGCATATATGAAATTAATGGGTACAACGAATCAAATACCGGTTTTC
  ATAGTAATGGGATTATACATTTGTATGTATGCTGTTTTTGCAGTGACGGCTTATAATCAT
  TCCAAGCGAACAATTAGACATTCCATATAA
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  1890
  

⊟Protein

⊟General

• locus tag: SACOL_RS03700 [old locus tag: SACOL0720 ]
• symbol: SACOL_RS03700
• description: ABC transporter permease
• length: 629
• theoretical pI: 10.0671
• theoretical MW: 71731.5
• GRAVY: 0.355008

⊟Function

• TIGRFAM:
  acidobacterial duplicated orphan permease (TIGR03434; HMM-score: 13.9)
• TheSEED: see SACOL0720
• PFAM:
  BPD_transp_1 (CL0404) FtsX; FtsX-like permease C-terminal (PF02687; HMM-score: 34.1)
  and 2 more
  no clan defined DUF6518; Family of unknown function (DUF6518) (PF20128; HMM-score: 13.7)
  FxsA; FxsA cytoplasmic membrane protein (PF04186; HMM-score: 8.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 10
• DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.9999
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0001
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.007787
  • TAT(Tat/SPI): 0.000247
  • LIPO(Sec/SPII): 0.002804
• predicted transmembrane helices (TMHMM): 10

⊟Accession numbers

• GI: 446065768 NCBI
• RefSeq: WP_000143623 NCBI
• UniProt: see SACOL0720

⊟Protein sequence

• MTFNEIIFKNFRQNLSHYAIYLFSLITSVVLYFSFVALKYAHKLNMTESYPIIKEGSQVGSYFLFFIIIAFLLYANVLFIKRRSYELALYQTLGLSKFNIIYILMLEQLLIFIITAILGIIIGIFGSKLLLMIVFTLLGIKEKVPIIFSLRAVFETLMLIGVAYFLTSAQNFILVFKQSISQMSKNNQVKETNHNKITFEEVVLGILGIVLITTGYYLSLNIVQYYDSIGTLMFILLSTVIGAYLFFKSSVSLVFKMVKKFRKGVISVNDVMFSSSIMYRIKKNAFSLTVMAIISAITVSVLCFAAISRASLSSEIKYTAPHDVTIKDQQKANQLASELNNQKIPHFYNYKEVIHTKLYKDNLFDVKAKEPYNVTITSDKYIPNTDLKRGQADLFVAEGSIKDLVKHKKHGKAIIGTKKHHVNIKLRKDINKIYFMTDVDLGGPTFVLNDKDYQEIRKYTKAKHIVSQFGFDLKHKKDALALEKAKNKVDKSIETRSEAISSISSLTGILLFVTSFLGITFLIAVCCIIYIKQIDETEDELENYSILRKLGFTQKDMARGLKFKIMFNFGLPLVIALSHAYFTSLAYMKLMGTTNQIPVFIVMGLYICMYAVFAVTAYNHSKRTIRHSI

⊟Experimental data

• experimentally validated: see SACOL0720
• protein localization: see SACOL0720
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications