PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS02800 [old locus tag: SACOL0547 ]
• pan locus tag^?: SAUPAN002244000
• symbol: SACOL_RS02800
• pan gene symbol^?: mfd
• synonym:
• product: transcription-repair coupling factor

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS02800 [old locus tag: SACOL0547 ]
• symbol: SACOL_RS02800
• product: transcription-repair coupling factor
• replicon: chromosome
• strand: +
• coordinates: 554052..557558
• length: 3507
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (554052..557558) NCBI
• BioCyc: SACOL_RS02800 BioCyc
• MicrobesOnline: see SACOL0547

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGACAATATTGACAACGCTTATAAAAGAAGATAATCATTTTCAAGACCTTAATCAGGTA
  TTTGGACAAGCAAACACACTAGTAACTGGTCTTTCCCCGTCAGCTAAAGTGACGATGATT
  GCTGAAAAATATGCACAAAGTAATCAACAGTTATTATTAATTACCAATAATTTATACCAA
  GCAGATAAATTAGAAACAGATTTACTTCAATTTATAGATGCTGAAGAATTGTATAAGTAT
  CCTGTGCAAGATATTATGACCGAAGAGTTTTCAACACAAAGCCCTCAACTGATGAGTGAA
  CGTATTAGAACTTTAACTGCGTTAGCTCAAGGTAAGAAAGGGTTATTTATCGTTCCTTTA
  AATGGTTTGAAAAAGTGGTTAACTCCTGTTGAAATGTGGCAAAATCACCAAATGACATTG
  CGTGTTGGTGAGGATATCGATGTGGACCAATTTCTTAACAAATTAGTTAATATGGGGTAC
  AAACGGGAATCCGTGGTATCGCATATTGGTGAATTCTCATTGCGAGGAGGTATTATCGAT
  ATCTTTCCGCTAATTGGGGAACCAATCAGAATTGAGCTATTTGATACCGAAATTGATTCT
  ATTCGGGATTTTGATGTTGAAACGCAGCGTTCCAAAGATAATGTTGAAGAAGTCGATATC
  ACAACTGCAAGTGATTATATCATTACTGAAGAAGTGATCAGCCATCTTAAAGAAGAGTTA
  AAAACTGCATATGAAAATACAAGACCCAAAATAGATAAATCAGTGCGCAATGATTTGAAA
  GAAACGTATGAAAGCTTTAAATTATTCGAAAGTACATACTTTGATCATCAAATACTACGT
  CGCTTAGTAGCGTTTATGTATGAAACACCTTCGACAATTATTGAGTATTTCCAAAAAGAT
  GCAATCATTGCAGTTGATGAATTTAATCGTATTAAAGAAACTGAAGAAAGTTTAACAGTA
  GAGCCTGATTCGTTTATTAGCAATATTATTGAAAGTGGTAATGGATTTATAGGACAAAGT
  TTTATAAAATATGATGATTTTGAAACATTGATTGAAGGCTATCCTGTCACTTATTTTTCA
  TTATTCGCTACAACAATGCCGATAAAACTAAATCATATTATTAAATTTTCATGTAAACCT
  GTCCAACAATTTTATGGGCAATATGACATTATGCGTTCTGAATTTCAACGATATGTTAAT
  CAAAACTATCATATCGTGGTTTTGGTCGAAACCGAAACTAAAGTTGAACGTATGCAAGCG
  ATGTTAAGTGAAATGCATATTCCATCAATAACAAAATTGCATCGCTCAATGTCATCGGGG
  CAAGCAGTGATTATTGAAGGCAGTTTATCTGAAGGATTTGAACTACCTGATATGGGATTA
  GTTGTCATTACTGAGCGTGAGCTTTTTAAATCAAAACAGAAAAAGCAACGAAAACGTACG
  AAAGCTATCTCAAATGCTGAAAAAATTAAGTCTTACCAAGATTTAAATGTGGGAGATTAT
  ATTGTTCATGTGCATCATGGTGTTGGTAGATATTTAGGTGTTGAGACGCTCGAAGTGGGG
  CAAACGCATCGTGATTATATTAAATTGCAATATAAAGGTACGGATCAACTATTTGTTCCA
  GTAGATCAAATGGATCAAGTTCAAAAATATGTAGCTTCGGAAGATAAGACGCCAAAATTA
  AATAAACTCGGTGGCAGTGAATGGAAAAAAACAAAAGCTAAAGTTCAACAAAGTGTTGAA
  GATATTGCTGAAGAGTTGATTGATTTATATAAAGAAAGAGAAATGGCAGAAGGTTATCAA
  TATGGGGAAGACACAGCTGAGCAAACAACATTTGAATTAGATTTTCCATATGAACTTACG
  CCTGACCAAGCTAAATCTATCGATGAAATTAAAGATGACATGCAAAAATCGCGTCCAATG
  GATCGCTTGCTATGTGGTGATGTTGGTTATGGTAAAACTGAAGTTGCAGTGAGAGCAGCA
  TTCAAAGCTGTAATGGAAGGAAAGCAGGTTGCATTTTTAGTTCCTACAACTATTTTAGCT
  CAGCAACATTATGAGACGTTAATTGAGCGTATGCAAGATTTTCCTGTTGAAATTCAATTA
  ATGAGTCGTTTTAGAACGCCTAAAGAGATAAAACAAACTAAGGAAGGACTTAAAACTGGA
  TTTGTTGACATAGTTGTTGGTACACACAAATTACTTAGTAAAGATATACAGTATAAAGAT
  TTAGGGCTGTTGATTGTAGATGAAGAACAACGATTTGGTGTACGCCATAAAGAGCGTATT
  AAAACATTAAAACATAATGTAGATGTACTAACATTGACTGCAACCCCAATACCTAGAACA
  TTGCATATGAGTATGCTAGGTGTGCGGGATTTGTCAGTGATTGAAACGCCGCCAGAAAAT
  CGTTTCCCAGTTCAAACATATGTATTAGAACAGAACATGAGTTTTATCAAAGAAGCTTTA
  GAAAGAGAACTATCCCGTGATGGCCAAGTGTTTTATCTTTATAATAAAGTGCAATCCATT
  TATGAAAAACGAGAACAACTCCAGATGTTAATGCCAGATGCTAACATTGCAGTTGCTCAT
  GGACAAATGACAGAGCGCGATTTAGAAGAAACGATGTTAAGTTTTATCAATAATGAATAT
  GATATTTTAGTAACGACGACGATTATTGAAACAGGTGTCGATGTCCCAAATGCAAATACT
  TTGATCATTGAAGATGCAGATCGCTTTGGATTGAGTCAGTTGTATCAATTAAGAGGTCGT
  GTTGGTCGTTCAAGTCGTATTGGTTATGCATACTTCTTACATCCAGCAAATAAGGTACTA
  ACTGAGACTGCAGAAGATCGATTACAAGCGATTAAAGAATTTACGGAGTTAGGCTCAGGA
  TTTAAGATTGCGATGCGTGATTTGAACATTCGTGGTGCTGGTAATTTGTTAGGTAAACAA
  CAGCACGGCTTTATTGATACAGTTGGATTTGATTTGTACAGTCAAATGTTAGAAGAAGCT
  GTAAATGAAAAACGTGGTATTAAGGAACCAGAATCTGAGGTGCCAGAAGTCGAAGTTGAT
  TTAAACTTGGATGCATATTTGCCAACAGAATATATTGCAAATGAACAAGCTAAAATTGAA
  ATTTATAAAAAGCTACGAAAAACTGAAACATTTGATCAAATTATCGACATTAAAGATGAA
  TTAATTGATCGTTTCAATGATTATCCTGTTGAAGTAGCACGTTTGCTTGATATAGTGGAA
  ATAAAAGTACACGCATTACATTCAGGTATCACGTTGATTAAAGATAAAGGGAAAATAATT
  GATATTCATTTATCTGTAAAAGCCACTGAAAATATTGATGGCGAAGTGCTGTTCAAAGCA
  ACACAACCTTTAGGTAGAACAATGAAGGTTGGTGTTCAAAATAATGCAATGACAATTACT
  TTAACGAAACAAAATCAATGGCTTGATAGTTTGAAGTTTTTAGTTAAGTGCATTGAAGAA
  AGTATGAGAATCAGTGATGAAGCATAA
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⊟Protein

⊟General

• locus tag: SACOL_RS02800 [old locus tag: SACOL0547 ]
• symbol: SACOL_RS02800
• description: transcription-repair coupling factor
• length: 1168
• theoretical pI: 4.86428
• theoretical MW: 134228
• GRAVY: -0.366353

⊟Function

• reaction:
  EC 3.6.4.-?  ExPASy
• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair transcription-repair coupling factor (TIGR00580; EC 3.6.1.-; HMM-score: 1127)
  and 5 more
  Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase RecG (TIGR00643; EC 3.6.4.12; HMM-score: 388.6)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair excinuclease ABC subunit B (TIGR00631; EC 3.1.25.-; HMM-score: 91.1)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair primosomal protein N' (TIGR00595; EC 3.6.1.-; HMM-score: 58.2)
  CRISPR-associated helicase Cas3 (TIGR01587; HMM-score: 46.3)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair reverse gyrase (TIGR01054; EC 3.6.4.12,5.99.1.3; HMM-score: 37.8)
• TheSEED: see SACOL0547
• PFAM:
  no clan defined CarD_CdnL_TRCF; CarD-like/TRCF domain (PF02559; HMM-score: 110.3)
  and 8 more
  TRCF; TRCF domain (PF03461; HMM-score: 82.8)
  P-loop_NTPase (CL0023) DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 67.5)
  Helicase_C; Helicase conserved C-terminal domain (PF00271; HMM-score: 63.4)
  ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 33.4)
  no clan defined Glyco_hydro_115; Glycosyl hydrolase family 115 (PF15979; HMM-score: 16.6)
  UB2H; Bifunctional transglycosylase second domain (PF14814; HMM-score: 16.3)
  P-loop_NTPase (CL0023) IstB_IS21; IstB-like ATP binding protein (PF01695; HMM-score: 14.5)
  AAA_22; AAA domain (PF13401; HMM-score: 13.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.006634
  • TAT(Tat/SPI): 0.001734
  • LIPO(Sec/SPII): 0.000867
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446076372 NCBI
• RefSeq: WP_000154227 NCBI
• UniProt: see SACOL0547

⊟Protein sequence

• MTILTTLIKEDNHFQDLNQVFGQANTLVTGLSPSAKVTMIAEKYAQSNQQLLLITNNLYQADKLETDLLQFIDAEELYKYPVQDIMTEEFSTQSPQLMSERIRTLTALAQGKKGLFIVPLNGLKKWLTPVEMWQNHQMTLRVGEDIDVDQFLNKLVNMGYKRESVVSHIGEFSLRGGIIDIFPLIGEPIRIELFDTEIDSIRDFDVETQRSKDNVEEVDITTASDYIITEEVISHLKEELKTAYENTRPKIDKSVRNDLKETYESFKLFESTYFDHQILRRLVAFMYETPSTIIEYFQKDAIIAVDEFNRIKETEESLTVEPDSFISNIIESGNGFIGQSFIKYDDFETLIEGYPVTYFSLFATTMPIKLNHIIKFSCKPVQQFYGQYDIMRSEFQRYVNQNYHIVVLVETETKVERMQAMLSEMHIPSITKLHRSMSSGQAVIIEGSLSEGFELPDMGLVVITERELFKSKQKKQRKRTKAISNAEKIKSYQDLNVGDYIVHVHHGVGRYLGVETLEVGQTHRDYIKLQYKGTDQLFVPVDQMDQVQKYVASEDKTPKLNKLGGSEWKKTKAKVQQSVEDIAEELIDLYKEREMAEGYQYGEDTAEQTTFELDFPYELTPDQAKSIDEIKDDMQKSRPMDRLLCGDVGYGKTEVAVRAAFKAVMEGKQVAFLVPTTILAQQHYETLIERMQDFPVEIQLMSRFRTPKEIKQTKEGLKTGFVDIVVGTHKLLSKDIQYKDLGLLIVDEEQRFGVRHKERIKTLKHNVDVLTLTATPIPRTLHMSMLGVRDLSVIETPPENRFPVQTYVLEQNMSFIKEALERELSRDGQVFYLYNKVQSIYEKREQLQMLMPDANIAVAHGQMTERDLEETMLSFINNEYDILVTTTIIETGVDVPNANTLIIEDADRFGLSQLYQLRGRVGRSSRIGYAYFLHPANKVLTETAEDRLQAIKEFTELGSGFKIAMRDLNIRGAGNLLGKQQHGFIDTVGFDLYSQMLEEAVNEKRGIKEPESEVPEVEVDLNLDAYLPTEYIANEQAKIEIYKKLRKTETFDQIIDIKDELIDRFNDYPVEVARLLDIVEIKVHALHSGITLIKDKGKIIDIHLSVKATENIDGEVLFKATQPLGRTMKVGVQNNAMTITLTKQNQWLDSLKFLVKCIEESMRISDEA

⊟Experimental data

• experimentally validated: see SACOL0547
• protein localization: see SACOL0547
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications