PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS02160 [old locus tag: SACOL0429 ]
• pan locus tag^?: SAUPAN001901000
• symbol: SACOL_RS02160
• pan gene symbol^?: metF
• synonym:
• product: bifunctional homocysteine S-methyltransferase/methylenetetrahydrofolate reductase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS02160 [old locus tag: SACOL0429 ]
• symbol: SACOL_RS02160
• product: bifunctional homocysteine S-methyltransferase/methylenetetrahydrofolate reductase
• replicon: chromosome
• strand: -
• coordinates: 434214..436055
• length: 1842
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (434214..436055) NCBI
• BioCyc: SACOL_RS02160 BioCyc
• MicrobesOnline: see SACOL0429

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAGTCAATTCCTCACACAATTGAAAGATAATGTTTTAGTAGCTGATGGCGCTATAGGA
  ACCATTTTATACTCTGAAGGATTAGACACCTGTCCAGAAGCATATAATCTTAGCCATCCA
  GATAAAGTTGAACGCATCCATCGTTCATATATTGAAGCCGGTGCTGATGTCATTCAAACC
  AATACTTATGGTGCAAATTTTGAAAAGTTAAAACGATTCGGTCTTGAAGATAAAGTTAAA
  GCAATACATCAAGCCGCCGTTCGCATCGCAAAAAAAGCAGCAAATAAAGATACGTATATA
  TTAGGCACAGTTGGTGGGTTTAGAGGTATCAAACAAGAGGATATCAGCTTACAAACTATT
  CTTTATCATACTGAAATTCAAATAGACACCTTAATTGAAGAAGGCGTTGACGCGCTACTT
  TTCGAAACGTATTACGACCTAGAAGAGTTAACAAATGTCATTTCACGAACGAGAAAGAAA
  TACGACATTCCAATCATTGCTCAATTAACCGCTTCAAACACAAATTACTTAGTTAATGGT
  CAGGCAATCAATGAAGGATTAAAACAACTCGTTCAATGTGGTGCAAACATCGTGGGACTC
  AATTGTCATCATGGCCCGCACCATATGCAAGAGTCTTTCACACATATTGAATTACCAGAG
  CACGCATTCTTATCTTGTTATCCAAATGCCAGCTTATTAGATATTGAAAATAGTGAATTT
  AAGTATAGTGACAATGCACAATATTTCGGTCAAGTTGCTCAAAATCTAATTCGCGAAGGT
  GTTCGTTTAATTGGTGGTTGCTGTGGTACAACGCCAGAGCACATCAAATTTATTAAAGAA
  TCTATTCAGACACTTAAACCTGTTAATGACAAAAAAGTGATTCCGATACCAACGAAAGCA
  CTTTTCAATCCATCTCAAAATAAAGTTAGACAATCATTAACATCTAAGGTTCAAGAACGT
  CCAACCGTTATTATCGAATTGGATACACCGAAACATTTAGACACGGATAGATTTTTTGAA
  AATATCGCTAAACTTGATAAAGCTAATGTAGATGCGGTAACACTCGCAGATAATTCATTG
  GCAACTGTCAGAATTAGCAATATTGCTGCTGCTAGCTTAATTAAGCAATATTACAATATT
  GAACCACTCGTACATATTACATGTCGAGACCGAAACTTAATCGGCTTGCAGTCCCATTTA
  CTTGGATTATCGCTCATTGGCGTTAACGAAATATTAGCCATAACTGGTGATCCTTCAAAA
  GTTGGTCACTTACCAGGTGCAACCAATGTCTATGATGTTAATTCTAAAGGATTAACTGAA
  CTCGCTCTAAGATTTAATCAAGGTATTAACACTGACGGTGATGCGCTGAAGAAACGTACA
  CACTTCAACATCGCTGGCGCCTTTAACCCTAATGTTCGTAAATTATATGGTGCCGTCAAA
  AGATTAGAGAAAAAGATAGAAAGCGGAATGTCTTATTTTATAACACAACCCGTGTACAGC
  AAAGAGAAAATCATTGAAATTTACCATGCCACTAAGCACTTGAACAAACCATTTTTCATA
  GGCATTATGCCTATCGCAAGTTACAAAAACGCACTCTTTTTGCATAATGAAGTGCCAGGA
  ATCAAGATGTCAGATGAAATTTTACAACAATTTGAAGCAGTTAAAGATGATAAAGCCAAA
  ACACGAGAACTAAGTCTTAAGCTTTCAAAGGATTTAATCGATACTGTTCATGAATATTTT
  AATGGTTTATACATTATCACACCGTTTCAAAATGTCGAAGATTCATTAGAACTTGCAGCA
  TACTCAAAATCTATTACTGCTCACAAGGAGGCAATATTATGA
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⊟Protein

⊟General

• locus tag: SACOL_RS02160 [old locus tag: SACOL0429 ]
• symbol: SACOL_RS02160
• description: bifunctional homocysteine S-methyltransferase/methylenetetrahydrofolate reductase
• length: 613
• theoretical pI: 6.98505
• theoretical MW: 68477.8
• GRAVY: -0.216803

⊟Function

• reaction:
  EC 2.1.1.10?  ExPASy
  Homocysteine S-methyltransferase S-methyl-L-methionine + L-homocysteine = 2 L-methionine
• TIGRFAM:
  Metabolism Amino acid biosynthesis Aspartate family methionine synthase (TIGR02082; EC 2.1.1.13; HMM-score: 154.8)
  and 2 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Folic acid methylenetetrahydrofolate reductase (TIGR00677; EC 1.5.1.20; HMM-score: 66.9)
  Metabolism Amino acid biosynthesis Aspartate family methylenetetrahydrofolate reductase [NAD(P)H] (TIGR00676; EC 1.5.1.20; HMM-score: 66.4)
• TheSEED: see SACOL0429
• PFAM:
  TIM_barrel (CL0036) S-methyl_trans; Homocysteine S-methyltransferase (PF02574; HMM-score: 208.6)
  and 3 more
  MTHFR; Methylenetetrahydrofolate reductase (PF02219; HMM-score: 99.9)
  no clan defined DUF6506; Family of unknown function (DUF6506) (PF20116; HMM-score: 13.8)
  Calcineurin (CL0163) PGA_cap; Bacterial capsule synthesis protein PGA_cap (PF09587; HMM-score: 13.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Zn²⁺
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9735
  • Cytoplasmic Membrane Score: 0.0137
  • Cell wall & surface Score: 0.0022
  • Extracellular Score: 0.0106
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.011577
  • TAT(Tat/SPI): 0.001511
  • LIPO(Sec/SPII): 0.0027
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 445999463 NCBI
• RefSeq: WP_000077318 NCBI
• UniProt: see SACOL0429

⊟Protein sequence

• MSQFLTQLKDNVLVADGAIGTILYSEGLDTCPEAYNLSHPDKVERIHRSYIEAGADVIQTNTYGANFEKLKRFGLEDKVKAIHQAAVRIAKKAANKDTYILGTVGGFRGIKQEDISLQTILYHTEIQIDTLIEEGVDALLFETYYDLEELTNVISRTRKKYDIPIIAQLTASNTNYLVNGQAINEGLKQLVQCGANIVGLNCHHGPHHMQESFTHIELPEHAFLSCYPNASLLDIENSEFKYSDNAQYFGQVAQNLIREGVRLIGGCCGTTPEHIKFIKESIQTLKPVNDKKVIPIPTKALFNPSQNKVRQSLTSKVQERPTVIIELDTPKHLDTDRFFENIAKLDKANVDAVTLADNSLATVRISNIAAASLIKQYYNIEPLVHITCRDRNLIGLQSHLLGLSLIGVNEILAITGDPSKVGHLPGATNVYDVNSKGLTELALRFNQGINTDGDALKKRTHFNIAGAFNPNVRKLYGAVKRLEKKIESGMSYFITQPVYSKEKIIEIYHATKHLNKPFFIGIMPIASYKNALFLHNEVPGIKMSDEILQQFEAVKDDKAKTRELSLKLSKDLIDTVHEYFNGLYIITPFQNVEDSLELAAYSKSITAHKEAIL

⊟Experimental data

• experimentally validated: see SACOL0429
• protein localization: see SACOL0429
• quantitative data / protein copy number per cell: see SACOL0429
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: T-box(Met) see SACOL0429

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: see SACOL0429

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications