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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS02150 [old locus tag: SACOL0427 ]
  • pan locus tag?: SAUPAN001899000
  • symbol: SACOL_RS02150
  • pan gene symbol?: mdh
  • synonym:
  • product: metal-dependent hydrolase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS02150 [old locus tag: SACOL0427 ]
  • symbol: SACOL_RS02150
  • product: metal-dependent hydrolase
  • replicon: chromosome
  • strand: -
  • coordinates: 431194..431946
  • length: 753
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (431194..431946) NCBI
  • BioCyc: SACOL_RS02150 BioCyc
  • MicrobesOnline: see SACOL0427

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGACACAATATCCTTTATGGCAACAATTAAATACTTTAAAACAAGCAACTTGGGTTGAT
    TTAACGCATACATTTGATCCGGAAATACCACGTTTTAGTGAGTTTGAAAAAGGCGAAGTT
    TCAACTTTATTCACGGTTAAAGATCATGGTTTCTATGTGCAACGTTGGAATATTGTAACG
    CAATATGGTACTCACATTGATGCACCAATTCACTTTGTCGAGAATAAACGTTATTTAGAA
    GACATTGATTTAAAAGAATTGGTATTGCCGTTAATTGTATTAGACTTTTCAACAGAAGTA
    GCGAACAACAATGATTTCATCGTAACACGTGCGCATATAGAAGCATGGGAAAAAGAGCAT
    GGTACTATTGAACCTGGCACTTTTGTTGCACTTCGTACCGATTGGTCAAAACGTTGGCCT
    AATATTGAAAAGTTTGAAAACAAAGATGCGAACGGACAACAACATGCACCGGGCTGGGGA
    TTAGATGCTTTGAAGTACTTAATTGAAGAACGTCGTGTTGAAGCTGTTGGTCATGAAACA
    TTCGATACCGATGCATCTGTGGACGTTGTAAAAAATGGTGATTTAGTTGGCGAACGTTAT
    ATTCTTGGTCAGGATAAATACCAAGTCGAGTTATTAACTAATTTAGATCAATTACCAACT
    CGTGGCGCCATCATTTACGCTATCAGCCCTAAACCAAAAGATGCACCTGGGTTCCCTGTT
    AGAGCATTTGCAATTAAACCATCAAATGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    753

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS02150 [old locus tag: SACOL0427 ]
  • symbol: SACOL_RS02150
  • description: metal-dependent hydrolase
  • length: 250
  • theoretical pI: 4.8323
  • theoretical MW: 28752.1
  • GRAVY: -0.4592

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Energy metabolism Amino acids and amines arylformamidase (TIGR03035; EC 3.5.1.9; HMM-score: 23.7)
  • TheSEED: see SACOL0427
  • PFAM:
    Leu-IlvD (CL0364) Cyclase; Putative cyclase (PF04199; HMM-score: 146.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9961
    • Cytoplasmic Membrane Score: 0.0002
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0036
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004083
    • TAT(Tat/SPI): 0.000231
    • LIPO(Sec/SPII): 0.00074
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTQYPLWQQLNTLKQATWVDLTHTFDPEIPRFSEFEKGEVSTLFTVKDHGFYVQRWNIVTQYGTHIDAPIHFVENKRYLEDIDLKELVLPLIVLDFSTEVANNNDFIVTRAHIEAWEKEHGTIEPGTFVALRTDWSKRWPNIEKFENKDANGQQHAPGWGLDALKYLIEERRVEAVGHETFDTDASVDVVKNGDLVGERYILGQDKYQVELLTNLDQLPTRGAIIYAISPKPKDAPGFPVRAFAIKPSND

⊟Experimental data[edit | edit source]

  • experimentally validated: see SACOL0427
  • protein localization: see SACOL0427
  • quantitative data / protein copy number per cell: see SACOL0427
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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