PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS00990 [old locus tag: SACOL0194 ]
• pan locus tag^?: SAUPAN001066000
• symbol: SACOL_RS00990
• pan gene symbol^?: malF
• synonym:
• product: maltose ABC transporter permease

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS00990 [old locus tag: SACOL0194 ]
• symbol: SACOL_RS00990
• product: maltose ABC transporter permease
• replicon: chromosome
• strand: +
• coordinates: 227788..229056
• length: 1269
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (227788..229056) NCBI
• BioCyc: SACOL_RS00990 BioCyc
• MicrobesOnline: see SACOL0194

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  
  ATGACGAAACGTAACCCTAAATTAGCGGCATTATTATCTGTTATACCTGGTTTGGGACAG
  TTTTATAATAAAAGACCCATTAAAGGGACGATATTTTTTATCTTTTTCATCAGTTTTATT
  TCTGTTTTTTATAGCTTTTTAAATATTGGTTTTTGGGGATTGTTCACATTAGGGACAGTA
  CCTAAGTTAGACGATTCTCGTGTCTTACTTGCACAAGGTATTATTTCTATCTTACTCGTT
  GCTTTCGCAATCATGCTATATATCATTAATATTTTAGATGCATATCGTAATGCTGAACGA
  TTTAATCGCAATGAGGAAATAAAGGATCCGAAGGCGCGTATGGTGGCAACATGGGACAAG
  ACGTTCCCATACTTACTAATCTCACCAGGTACATTCTTATTGATATTTGTAGTTGTATTT
  CCATTAATATTTATGTTTGGAGTAGCATTTACAAATTACAATTTATACAACGCGCCTCCG
  AGACACACATTAGAATGGGTTGGTTTAGATAACTTTAAAACGTTATTCACAATTGGCGTT
  TGGCGTAAAACATTTTTCAGTGTTATTACTTGGACATTAGTATGGACGCTTGTTGCAACG
  ACACTTCAAATTGCATTAGGGCTGTTTTTGGCAATTATTGTAAATCACCCTGTCGTCAAA
  GGTAAGAAATTTATCCGTACTGTGTTAATCCTACCTTGGGCTGTACCATCATTTGTGACA
  ATTTTAATATTTGTAGCGTTATTTAATGATGAATTTGGTGCGATAAATAATGATATTTTG
  CAACCTTTATTAGGTGTAGCACCAGCATGGTTAAGTGATCCGTTTTGGGCAAAAGTGGCA
  TTAATCGGCATTCAAGTATGGCTTGGATTCCCATTTGTCTTTGCACTGTTCACTGGAGTA
  CTGCAAAGTATTTCATCAGATTGGTACGAAGCAGCAGATATGGATGGTGCGTCTAGTTGG
  CAAAAGTTTAGAAACATCACATTCCCGCATGTCATTTACGCCACAGCGCCATTGTTAATT
  ATGCAATATGCAGGTAATTTCAATAATTTTAATCTTATTTATCTATTTAATAAAGGCGGT
  CCACCAGTGTCAGGGCAGAATGCTGGTAGTACAGATATCTTGATATCTTGGGTGTATAAT
  CTGACATTTGAGTTTAACAACTTCAACATGGGTGCAGTTGTGTCATTAATTATTGGATTT
  ATTGTTGCTATTGTCGCATTTATTCAATTCAGACGTACAAGTACGTTTAAAGATGAGGGA
  GGTTTATAA
  60
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  1269
  

⊟Protein

⊟General

• locus tag: SACOL_RS00990 [old locus tag: SACOL0194 ]
• symbol: SACOL_RS00990
• description: maltose ABC transporter permease
• length: 422
• theoretical pI: 9.9436
• theoretical MW: 47660.8
• GRAVY: 0.659953

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Amino acids, peptides and amines putative 2-aminoethylphosphonate ABC transporter, permease protein (TIGR03262; HMM-score: 57.6)
  Metabolism Transport and binding proteins Anions sulfate ABC transporter, permease protein CysW (TIGR02140; HMM-score: 47.2)
  Metabolism Transport and binding proteins Anions sulfate ABC transporter, permease protein (TIGR00969; HMM-score: 46.3)
  Metabolism Transport and binding proteins Anions sulfate ABC transporter, permease protein CysT (TIGR02139; HMM-score: 46.3)
  and 6 more
  Metabolism Transport and binding proteins Anions molybdate ABC transporter, permease protein (TIGR02141; HMM-score: 36.2)
  NifC-like ABC-type porter (TIGR01581; HMM-score: 34.3)
  2-aminoethylphosphonate ABC transport system, membrane component PhnV (TIGR03255; HMM-score: 21.3)
  2-aminoethylphosphonate ABC transporter, permease protein (TIGR03226; HMM-score: 17.9)
  Metabolism Transport and binding proteins Anions phosphonate ABC transporter, permease protein PhnE (TIGR01097; HMM-score: 16.3)
  Cellular processes Cellular processes Chemotaxis and motility flagellar biosynthetic protein FlhB (TIGR00328; HMM-score: 12.1)
• TheSEED: see SACOL0194
• PFAM:
  BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 91.1)
  and 1 more
  no clan defined TM_PBP2_N; N-terminal of TM subunit in PBP-dependent ABC transporters (PF16296; HMM-score: 7.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 8
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002227
  • TAT(Tat/SPI): 0.000229
  • LIPO(Sec/SPII): 0.016777
• predicted transmembrane helices (TMHMM): 8

⊟Accession numbers

• GI: 446089033 NCBI
• RefSeq: WP_000166888 NCBI
• UniProt: see SACOL0194

⊟Protein sequence

• MTKRNPKLAALLSVIPGLGQFYNKRPIKGTIFFIFFISFISVFYSFLNIGFWGLFTLGTVPKLDDSRVLLAQGIISILLVAFAIMLYIINILDAYRNAERFNRNEEIKDPKARMVATWDKTFPYLLISPGTFLLIFVVVFPLIFMFGVAFTNYNLYNAPPRHTLEWVGLDNFKTLFTIGVWRKTFFSVITWTLVWTLVATTLQIALGLFLAIIVNHPVVKGKKFIRTVLILPWAVPSFVTILIFVALFNDEFGAINNDILQPLLGVAPAWLSDPFWAKVALIGIQVWLGFPFVFALFTGVLQSISSDWYEAADMDGASSWQKFRNITFPHVIYATAPLLIMQYAGNFNNFNLIYLFNKGGPPVSGQNAGSTDILISWVYNLTFEFNNFNMGAVVSLIIGFIVAIVAFIQFRRTSTFKDEGGL

⊟Experimental data

• experimentally validated:
• protein localization: see SACOL0194
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: MalR see SACOL0194

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications