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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL2721 [new locus tag: SACOL_RS14230 ]
- pan locus tag?: SAUPAN006459000
- symbol: nixA
- pan gene symbol?: nixA
- synonym:
- product: high-affinity nickel-transport protein
⊟Additional information (user-provided)[edit | edit source]
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL2721 [new locus tag: SACOL_RS14230 ]
- symbol: nixA
- product: high-affinity nickel-transport protein
- replicon: chromosome
- strand: +
- coordinates: 2793058..2794110
- length: 1053
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3237622 NCBI
- RefSeq: YP_187507 NCBI
- BioCyc: see SACOL_RS14230
- MicrobesOnline: 914187 MicrobesOnline
⊟Phenotype[edit | edit source]
⊟Additional information (user-provided)[edit | edit source]
⊟DNA sequence[edit | edit source]
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1021ATGATTAAATATCATGTTATGAAAGAAGGATATATTTTGACTGTTTTTAAAAATGAGCGC
TTAAGCTGGTTACCATACATAGCTATTGTCATTTTGTTACACGTTATTGGGTTTAGTTTT
TTATGGATTGCTGGAAAAGACCATCATATCTTATTTGGTATGGGGATTCTTGCATATACA
TTAGGTTTGCGTCATGCATTTGATGCAGATCACATTGCTGCAATAGATAATACGGTTCGC
AAATTATTACAGCAACGCAAAGATCCATCTGGTGTGGGTTTCTATTTTTCAATTGGACAT
TCATCTGTCGTATTTTTAATGGCTGTTTTTTTAGGGGTATCTGTAAAATGGGCTAAAGAT
GAATTACCGCATTTCCAAGATATTGGTGGAACGATTGGTACACTAGTTTCAGGTTTCTTT
TTAGTGCTTATCGGTGTGTTGAATCTAATTATTTTAATCTCTTTAATTAACTTGTTCGCT
AAATTACGTCGTGAACACATTGAAGAAGCTGAAGTCGATGCATTACTTGAATCTAGAGGA
TTGGTTTCTCGATTTGTAGGACCTTATTTCAAATTAATCACGCGTAGTTGGCACGTATTG
CCACTTGGCTTTTTATTTGGACTTGGTTTTGATACAGCTAGTGAAATTGCGTTACTCGCT
CTTTCTTCAGGCGCATCACAACAAGCCATTTCATTTATCGGAATCTTATCTTTACCAATT
TTATTCGCATCAGGTATGAGTTTATTGGATACATTAGATGGTGTTGTAATGAAGTATGCC
TATAATTGGGCATTCTTCAATCCTATTCGCAAAATCTATTACAATATAACGATTACTGCG
ATATCAGTCATGGCAGCATTAGTGATTGGGATGATTGAATTGCTACAAATTCTTGCTGAT
AAGTTAGATTTACATGGTGCGTTTTGGGCATTCATTGGTTCGATCGAATTTGATTATTTA
GGCTATATTTTAGTTGCATTATTTTTAATTACTTGGCTTATTTCAAGTTTAATTTGGAAG
TTTGGTCGCATAGAACACAAATGGTCTAGATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL2721 [new locus tag: SACOL_RS14230 ]
- symbol: NixA
- description: high-affinity nickel-transport protein
- length: 350
- theoretical pI: 8.66941
- theoretical MW: 39305.3
- GRAVY: 0.743714
⊟Function[edit | edit source]
- TIGRFAM: Transport and binding proteins Cations and iron carrying compounds transition metal uptake transporter, Ni2+-Co2+ transporter (NiCoT) family (TIGR00802; HMM-score: 380.8)
- TheSEED :
- HoxN/HupN/NixA family nickel/cobalt transporter
- PFAM: LysE (CL0292) NicO; High-affinity nickel-transport protein (PF03824; HMM-score: 377)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 8
- DeepLocPro: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 0.9999
- Cell wall & surface Score: 0
- Extracellular Score: 0.0001
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.000863
- TAT(Tat/SPI): 0.000086
- LIPO(Sec/SPII): 0.000757
- predicted transmembrane helices (TMHMM): 8
⊟Accession numbers[edit | edit source]
⊟Additional information (user-provided)[edit | edit source]
⊟Protein sequence[edit | edit source]
- MIKYHVMKEGYILTVFKNERLSWLPYIAIVILLHVIGFSFLWIAGKDHHILFGMGILAYTLGLRHAFDADHIAAIDNTVRKLLQQRKDPSGVGFYFSIGHSSVVFLMAVFLGVSVKWAKDELPHFQDIGGTIGTLVSGFFLVLIGVLNLIILISLINLFAKLRREHIEEAEVDALLESRGLVSRFVGPYFKLITRSWHVLPLGFLFGLGFDTASEIALLALSSGASQQAISFIGILSLPILFASGMSLLDTLDGVVMKYAYNWAFFNPIRKIYYNITITAISVMAALVIGMIELLQILADKLDLHGAFWAFIGSIEFDYLGYILVALFLITWLISSLIWKFGRIEHKWSR
⊟Experimental data[edit | edit source]
- experimentally validated:
- protein localization: Integral membrane [1]
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator: SigB* (activation) regulon
SigB* (sigma factor) controlling a large regulon involved in stress/starvation response and adaptation; [2] other strains
⊟Additional information (user-provided)[edit | edit source]
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Additional information (user-provided)[edit | edit source]
⊟Other information (user-provided)[edit | edit source]
You can add further information about the gene and protein here. [edit]
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p) - ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
J Bacteriol: 2004, 186(13);4085-99
[PubMed:15205410] [WorldCat.org] [DOI] (P p)