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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL2582 [new locus tag: SACOL_RS13525 ]
- pan locus tag?: SAUPAN006232000
- symbol: SACOL2582
- pan gene symbol?: oatA
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL2582 [new locus tag: SACOL_RS13525 ]
- symbol: SACOL2582
- product: hypothetical protein
- replicon: chromosome
- strand: -
- coordinates: 2645664..2647475
- length: 1812
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3237206 NCBI
- RefSeq: YP_187374 NCBI
- BioCyc: see SACOL_RS13525
- MicrobesOnline: 914053 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1801ATGGATACAAAAGACTTTAAACGTTTAGAAAAAATGTATTCCCCGCGATACTTACCTGGA
TTAGATGGATTGAGGGCATTCGCAGTTATAGGAATCATTATTTATCACTTGAATGCACAA
TGGTTAAGTGGGGGCTTTTTAGGAGTAGATACATTCTTCGTTATTTCAGGTTATTTAATA
ACAAGTTTGTTGATAAGTGAGTATTATCGGACGCAAAAAATCGATTTGCTAGAGTTTTGG
AAGCGACGATTGAAACGACTCATTCCGGCAGTGTTATTTTTAATCTGTGTCGTGCTTACG
TTCACACTGATATTTAAACCGGAATTGATTATACAAATGAAACGAGATGCTATTGCAGCT
ATATTCTATGTTTCAAACTGGTGGTACATTTCACAGAATGTAGATTATTTTAACCAATTT
GCTATTGAACCACTCAAACATTTATGGTCTTTAGCCATTGAAGAACAATTTTACTTGCTT
TTCCCATTGGTTATCACGTTCTTATTACATAGATTTAAACCGAGAAATATTATTCAAACG
CTATTTATTGTATCGTTGATTTCTTTAGGACTTATGATAGTGATTCATTTCATCACTGGA
GATAATTCACGTGTGTATTTTGGGACAGATACACGACTGCAAACTTTATTGCTTGGTTGT
ATATTAGCATTTATTTGGCCTCCGTTTGCTTTGAAAAAAGATATTTCTAAAAAGATTGTC
GTATCATTAGATATTATAGGGATATCTGGTTTTGCGGTTCTAATGACTTTGTTCTTTATA
GTTGGAGACCAAGATCAATGGATCTATAATGGAGGATTTTACATTATATCATTTGCAACT
TTATTCATTATTGCAATTGCGGTACATCCTTCTAGTTTATTTGCTAAATTTTTAAGTATG
AAACCTTTACTAATTATAGGTAAACGATCATATAGCTTATACTTATGGCATTATCCTATC
ATTGTTTTTGTGAACAGTTATTACGTACAAGGACAAATACCGGTATACGTTTATATTATA
GAAATTTTGTTAACAGCGTTAATGGCTGAAATTTCGTATCGCTTTATTGAAACACCTATA
CGTAAAAAAGGATTTAAAGCTTTTGCATTTTTACCTAAAAAGAAGGGGCAATTTGCTAGA
ACAGTGTTAGTTATCCTATTATTGGTTCCGTCTATCGTTGTGCTCAGTGGACAGTTTGAT
GCACTTGGCAAACAACATGAAGCCGAGAAGAAAGAGAAGAAGACGGAATTTAAAACAACG
AAGAAAAAAGTCGTTAAAAAAGATAAGCAAGAGGATAAGCAGACAGCGAATAGCAAAGAG
GATATTAAAAAGTCATCACCACTATTAATTGGTGACTCGGTCATGGTGGATATTGGTAAT
GTCTTTACTAAGAAAATACCAAATGCACAAATTGATGGTAAAGTTGGACGGCAACTCGTT
GATGCTACACCAATTGTGAAATCGCAATATAAAGACTATGCTAAAAAAGGTCAAAAAGTT
GTAGTAGAGCTTGGTACAAATGGGGCATTTACGAAAGATCAATTAAATGAACTATTGGAT
AGTTTTGGAAAAGCAGACATATATTTAGTTTCTATTAGAGTACCTAGAGATTATGAAGGT
AGAATAAATAAATTAATTTATGAGGCAGCTGAAAAGCGCTCTAATGTACATCTAGTCGAT
TGGTATAAAGCTTCTGCAGGTCATCCGGAATACTTTGCATATGACGGTATTCACTTAGAA
TATGCAGGTAGTAAAGCGCTGACTGATTTGATTGTAAAAACGATGGAAACACATGCTACA
AATAAGAAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL2582 [new locus tag: SACOL_RS13525 ]
- symbol: SACOL2582
- description: hypothetical protein
- length: 603
- theoretical pI: 10.0029
- theoretical MW: 69098.1
- GRAVY: 0.222554
⊟Function[edit | edit source]
- reaction: EC 2.3.1.-? ExPASy
- TIGRFAM: Cellular processes Sporulation and germination stage III sporulation protein AF (TIGR02896; HMM-score: 5.1)
- TheSEED :
- O-acetyltransferase
- YrhL-like hydrolase
- PFAM: Acyl_transf_3 (CL0316) Acyl_transf_3; Acyltransferase family (PF01757; HMM-score: 93.7)and 2 moreSGNH_hydrolase (CL0264) Lipase_GDSL_2; GDSL-like Lipase/Acylhydrolase family (PF13472; HMM-score: 16.9)no clan defined DisA-linker; DisA bacterial checkpoint controller linker region (PF10635; HMM-score: 12.5)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 11
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: -1
- N-terminally Anchored Score: 0
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.002962
- TAT(Tat/SPI): 0.000138
- LIPO(Sec/SPII): 0.001129
- predicted transmembrane helices (TMHMM): 11
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MDTKDFKRLEKMYSPRYLPGLDGLRAFAVIGIIIYHLNAQWLSGGFLGVDTFFVISGYLITSLLISEYYRTQKIDLLEFWKRRLKRLIPAVLFLICVVLTFTLIFKPELIIQMKRDAIAAIFYVSNWWYISQNVDYFNQFAIEPLKHLWSLAIEEQFYLLFPLVITFLLHRFKPRNIIQTLFIVSLISLGLMIVIHFITGDNSRVYFGTDTRLQTLLLGCILAFIWPPFALKKDISKKIVVSLDIIGISGFAVLMTLFFIVGDQDQWIYNGGFYIISFATLFIIAIAVHPSSLFAKFLSMKPLLIIGKRSYSLYLWHYPIIVFVNSYYVQGQIPVYVYIIEILLTALMAEISYRFIETPIRKKGFKAFAFLPKKKGQFARTVLVILLLVPSIVVLSGQFDALGKQHEAEKKEKKTEFKTTKKKVVKKDKQEDKQTANSKEDIKKSSPLLIGDSVMVDIGNVFTKKIPNAQIDGKVGRQLVDATPIVKSQYKDYAKKGQKVVVELGTNGAFTKDQLNELLDSFGKADIYLVSIRVPRDYEGRINKLIYEAAEKRSNVHLVDWYKASAGHPEYFAYDGIHLEYAGSKALTDLIVKTMETHATNKK
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas
- protein localization: Integral membrane [1] [2] [3] [4]
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS One: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e) - ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
J Proteome Res: 2010, 9(3);1579-90
[PubMed:20108986] [WorldCat.org] [DOI] (I p) - ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
J Proteome Res: 2011, 10(4);1657-66
[PubMed:21323324] [WorldCat.org] [DOI] (I p) - ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p)
⊟Relevant publications[edit | edit source]
Agnieszka Bera, Silvia Herbert, Andreas Jakob, Waldemar Vollmer, Friedrich Götz
Why are pathogenic staphylococci so lysozyme resistant? The peptidoglycan O-acetyltransferase OatA is the major determinant for lysozyme resistance of Staphylococcus aureus.
Mol Microbiol: 2005, 55(3);778-87
[PubMed:15661003] [WorldCat.org] [DOI] (P p)