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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2013 [new locus tag: SACOL_RS10510 ]
  • pan locus tag?: SAUPAN005240000
  • symbol: SACOL2013
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2013 [new locus tag: SACOL_RS10510 ]
  • symbol: SACOL2013
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2073768..2074205
  • length: 438
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    TTGTTTATTTCTCTAGCTATTTGGTCAATAAATTCGATGGGTAATAAACAATGCATTAGT
    TTGAATTTATTGCTTTCGGATGATACTTTCGCAGTAATTTCAGTAATGACAACTTTGGGA
    ACTGCTATTTTAGGAGGTGTAGCACCTTCAATATATGGTATTTTTACATCGCCTATAGAT
    AAAAAAAGTAGTTTTAGGGCTATTAGCGATTGGGAGCAAGAACAAAGAAATAATGTTAAA
    GATCTGATGATGCTATTACCATTTACATTACTTAGTATTTTAATTGTGATTTTTATTTTT
    CAGTTTGCATATTCTAAACTATTTAATACGATAGCTATTATAATATTTATTATAATACTT
    GTTGTTATGTTTTTTTTAATTTTATTAATCTGTTATAAAATTACTGTGAAGATATGCAGA
    TATTTTTCAAAAGAATAG
    60
    120
    180
    240
    300
    360
    420
    438

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2013 [new locus tag: SACOL_RS10510 ]
  • symbol: SACOL2013
  • description: hypothetical protein
  • length: 145
  • theoretical pI: 8.84397
  • theoretical MW: 16467.8
  • GRAVY: 0.993793

⊟Function[edit | edit source]

  • TIGRFAM:
    MSEP-CTERM protein (TIGR04286; HMM-score: 10.6)
  • TheSEED: data available for NCTC8325, Newman
  • PFAM:
    no clan defined EIID-AGA; PTS system mannose/fructose/sorbose family IID component (PF03613; HMM-score: 15)
    MscS_TM; Mechanosensitive ion channel inner membrane domain 1 (PF12794; HMM-score: 14.4)
    DUF3671; Fam-L, Fam-M like protein (PF12420; HMM-score: 12.6)
    TRP; Transient receptor potential (TRP) ion channel (PF06011; HMM-score: 12.5)
    and 6 more
    Virul_fac_BrkB; Virulence factor BrkB (PF03631; HMM-score: 11.6)
    DUF3043; Protein of unknown function (DUF3043) (PF11241; HMM-score: 10.8)
    Ac76; Orf76 (Ac76) (PF05814; HMM-score: 9.2)
    DUF6480; Family of unknown function (DUF6480) (PF20088; HMM-score: 9)
    Yip1 (CL0112) DUF1129; Protein of unknown function (DUF1129) (PF06570; HMM-score: 8.9)
    no clan defined DUF6366; Family of unknown function (DUF6366) (PF19893; HMM-score: 8.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 3
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0004
    • Cytoplasmic Membrane Score: 0.9515
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.0481
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.049595
    • TAT(Tat/SPI): 0.001817
    • LIPO(Sec/SPII): 0.0031
  • predicted transmembrane helices (TMHMM): 3

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MFISLAIWSINSMGNKQCISLNLLLSDDTFAVISVMTTLGTAILGGVAPSIYGIFTSPIDKKSSFRAISDWEQEQRNNVKDLMMLLPFTLLSILIVIFIFQFAYSKLFNTIAIIIFIIILVVMFFLILLICYKITVKICRYFSKE

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigB* (activation) regulon
    SigB*(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation;  [1]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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