From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1821 [new locus tag: SACOL_RS09335 ]
  • pan locus tag?: SAUPAN004460000
  • symbol: SACOL1821
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1821 [new locus tag: SACOL_RS09335 ]
  • symbol: SACOL1821
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1876441..1877943
  • length: 1503
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGCGTGTAGCAATAATAGGCATGGGAACTGCTGGTGTAAGTGTATTGAGAGAGTTAGTT
    AAGCACCCGAAGTTTAATCAATTAGATATAGATTTATACGATGATAAAGTAAATATGGGA
    CAGGGCGTACCATTTCAAAATGATAGTTCTGAGCTGCTAATTAATATGCCTTCGAAAAAG
    ATGAGTTTAAATTTAGATGATGAGACTGAATTTTGGAAGTGGTATAAACAACAAACTGAT
    TTTAATTTTGATGAACCTGCATATTTACCGAGATTTGTATTCGGACATTATATGAAATCT
    TATTTATCTATGTTCACGAAAAAATATCCAAATATATCAACTAATTATAATAAAGTTCAA
    GAGATTTATACAAATTCTAATATCGATGAAACGAATTTAACTTATTACATATGTACTACA
    AATTCAGGACAATCATGGCAAGCGTACGATTATGTATTTTTAACATGTGGTACATTTGCC
    TACCATGATCCATATAACTTAAAGGGTAAAAAAGGATATATCGCTACGCCTTATCCAACA
    TATAATACGTTAGACGAAGTAAATGAACTGGACGATATTGCGATTATTGGTACAGGCCTT
    GCCAGTTTAGATGTTGTCCGTTATGTTGCTGCTCATCATCCAAAATTACCAATTACTATG
    ACGAGTCGTTCTGCTCATTTACCAAGTGTAAGAGGTACAATGATAGACGTGACATTCAAG
    TACTTAACTAAAGACAAATTAAATGACATTAAAAAACATCATTTCGGCAATGCACCGCTT
    GATACTTTAGTTTCTTTATTTTTAAAAGAATGTGCTGATTATGACATTGATTTTAAAAAA
    TTAGTACATAGACGTACAGGAAATCATATTGCAGATTTGAAGTATGATTTAGCGCGTCCA
    ACAGAAATGGGCATTTTCCAAAGTATGATTGAACATTTGAAAGAGAATTTAAATTGGATA
    TGGAATAGCTTAAGTATTGAAGACCAACATCAATTTAATCAAAAATACTCAAAGATGATT
    CAATTAAATTCAAATCCAATGCCTCCAAGAACTGCTGAACTAATTATTGAATTAATAGAA
    AATAAGTCTTTAATTTTGAAAAAAGATTTAGAAGATGTGAAACATGATGGCAAGTTGTAT
    TATTTTTCATACAAAAACCAAGAATCAGTAGACATATATAATGTTGTCATTAACGCGACA
    GGTGCAAAAAGTCATTTAAATGAATTGGATCAAGATGATCAATTAATTAGAAATCTTGAA
    AATAGACAAATTGTACAAGCACACCCAATGGGAGGCATACAAATCATTCCGGAAACCAAT
    CAAGTTATTAGTCCAAGGTTTGGAACATTAACAAATATGATTGCGATAGGTCAAATGACA
    AACGGTGTTAATAAACTACGAAACGGTGTGAAAATGATTGTAGAACAAGTTGCGCATACA
    GTATCTCAATTATATGATGCTTTAGAATCGAATGAGCAACAACAGCGCAGTGACAATCAA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1503

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1821 [new locus tag: SACOL_RS09335 ]
  • symbol: SACOL1821
  • description: hypothetical protein
  • length: 500
  • theoretical pI: 6.41197
  • theoretical MW: 57563
  • GRAVY: -0.475

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain (TIGR03197; HMM-score: 15.4)
    salicylate 1-monooxygenase (TIGR03219; EC 1.14.13.1; HMM-score: 14.9)
  • TheSEED  :
    • COG4529
  • ⊞PFAM:
    NADP_Rossmann (CL0063) NAD_binding_9; FAD-NAD(P)-binding (PF13454; HMM-score: 111.9)
    and 8 more
    Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 30)
    Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 25.4)
    MurF-HprK_N (CL0365) MurD-like_N; Mur ligase MurD-like, N-terminal domain (PF21799; HMM-score: 17.8)
    NADP_Rossmann (CL0063) Lys_Orn_oxgnase; L-lysine 6-monooxygenase/L-ornithine 5-monooxygenase (PF13434; HMM-score: 17)
    DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 15.2)
    Thi4; Thi4 family (PF01946; HMM-score: 15)
    no clan defined Ribophorin_II_2nd; Ribophorin II second domain (PF23861; HMM-score: 13.4)
    NADP_Rossmann (CL0063) Sacchrp_dh_NADP; Saccharopine dehydrogenase NADP binding domain (PF03435; HMM-score: 12.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.7573
    • Cytoplasmic Membrane Score: 0.2303
    • Cell wall & surface Score: 0.0004
    • Extracellular Score: 0.012
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 0.83
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.016499
    • TAT(Tat/SPI): 0.000305
    • LIPO(Sec/SPII): 0.005268
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRVAIIGMGTAGVSVLRELVKHPKFNQLDIDLYDDKVNMGQGVPFQNDSSELLINMPSKKMSLNLDDETEFWKWYKQQTDFNFDEPAYLPRFVFGHYMKSYLSMFTKKYPNISTNYNKVQEIYTNSNIDETNLTYYICTTNSGQSWQAYDYVFLTCGTFAYHDPYNLKGKKGYIATPYPTYNTLDEVNELDDIAIIGTGLASLDVVRYVAAHHPKLPITMTSRSAHLPSVRGTMIDVTFKYLTKDKLNDIKKHHFGNAPLDTLVSLFLKECADYDIDFKKLVHRRTGNHIADLKYDLARPTEMGIFQSMIEHLKENLNWIWNSLSIEDQHQFNQKYSKMIQLNSNPMPPRTAELIIELIENKSLILKKDLEDVKHDGKLYYFSYKNQESVDIYNVVINATGAKSHLNELDQDDQLIRNLENRQIVQAHPMGGIQIIPETNQVISPRFGTLTNMIAIGQMTNGVNKLRNGVKMIVEQVAHTVSQLYDALESNEQQQRSDNQ

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • ⊞regulator: SigB* (activation) regulon

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  3. ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SACOL1821&oldid=94009"