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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL1443 [new locus tag: SACOL_RS07360 ]
- pan locus tag?: SAUPAN003825000
- symbol: brnQ3
- pan gene symbol?: brnQ3
- synonym:
- product: branched-chain amino acid transport system II carrier protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL1443 [new locus tag: SACOL_RS07360 ]
- symbol: brnQ3
- product: branched-chain amino acid transport system II carrier protein
- replicon: chromosome
- strand: -
- coordinates: 1453630..1454973
- length: 1344
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3236551 NCBI
- RefSeq: YP_186295 NCBI
- BioCyc: see SACOL_RS07360
- MicrobesOnline: 912901 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1321ATGAATAAAAATACATGGGTCATTGGGTTTACGCTCTTCGCTATGTTTTTTGGCGCAGGC
AATCTAATTTTTCCTCCTAATCTCGGATTGGATAGTGGTCAATTTTTCTGGCCCGCCATT
TTAGCGTTTGTTCTAACTGGGATTGGTTTACCATTATTAGGTGTGATTGTAGGTGCACTT
GATAAAGAAGGATATATTGGCGCATTAAATAAAATTTCACCTAAATTTTCAATATTGTTC
TTAATCATCATTTATTTGACTATAGGACCACTTTTTGCAATACCTAGAACTGCATCTACA
TCTTTTGAAATGACAATTACACCAATTATACATAGCAATAGTAGTATCGCTTTATTTATA
TTTACGATTATCTACTTCATAGTCGTTTTGTATATTTGTTTAAATCCATCTAAGTTAATC
GATCGTATTGGTTCATTATTAACACCATTATTATTGATTACTATTTTAGCGATGATTATT
AAAGGATACTTAGACTTTAGCGGTAATAGTGCTGGAAAGGGCAATGAAGCACTATATCAT
TCTAATTTTTCAAGTTTTGCTGAAGGCTTTACACAAGGCTATTTAACAATGGATGCCATT
GCAGCAATTGCTTTTTCAATGATTGTTGTTAATGCAGTAAAACTAACAGGCATTACTAAA
ACAAATCAAATATTCAAACAAACTTTGACTGCTGGTTTAATTGCAGCCGTAGCTTTAATT
TTCATATATATTTCATTAGGTTATATTGGTAATCATATGCCAGTAAGTGACATGACGTTA
GATCAATTGAAATCCAAAGATCGAAACATTGGGACATATTTATTAACGACAATGGCTTCA
ACAGGATTTGGTTCATTCGGAAAATATTTATTGGGCATCATTGTGGCGCTGGCATGTCTA
ACTACAGCATGCGGGCTTATTGTTGCAGTTTCTGAATATTTCCATAGAATCGTACCTAAA
GTATCATACAAAGCATTTGTATTAGTTTTCATTTTAATGAGTTTTATTATTGCTAACCAA
GGTTTAAATGCTGTTATCTCAATGTCAATTCCGGTATTAAGCATTGTATACCCAGTAGCA
ATAACTGTTGTATTATTAATTTTAATTGCCAAATTCATACCGACAAAACGCATTTCACAA
CAAATTCCAGTTATTATCGTATTTATATTGTCGATTTTCAGTGTTATTAGTAAGTTAGGT
TGGCTGAAAATTAACTTTATAGAATCATTGCCTCTAAGAGCGTATTCTTTAGAGTGGTTC
CCAGTAGCAATTATTGCAACGATATTAGGCTATCTAGTCGGCATATTTGTAAAACAAGAT
CCAATTAAATATCAACAGGAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL1443 [new locus tag: SACOL_RS07360 ]
- symbol: BrnQ3
- description: branched-chain amino acid transport system II carrier protein
- length: 447
- theoretical pI: 9.68796
- theoretical MW: 48814.2
- GRAVY: 0.975839
⊟Function[edit | edit source]
- TIGRFAM: Transport and binding proteins Amino acids, peptides and amines branched-chain amino acid transport system II carrier protein (TIGR00796; HMM-score: 520.7)
- TheSEED :
- Na(+)-dependent branched-chain amino acid transporter
- PFAM: APC (CL0062) Branch_AA_trans; Branched-chain amino acid transport protein (PF05525; HMM-score: 455)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 12
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: 0
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.048743
- TAT(Tat/SPI): 0.00078
- LIPO(Sec/SPII): 0.12828
- predicted transmembrane helices (TMHMM): 12
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MNKNTWVIGFTLFAMFFGAGNLIFPPNLGLDSGQFFWPAILAFVLTGIGLPLLGVIVGALDKEGYIGALNKISPKFSILFLIIIYLTIGPLFAIPRTASTSFEMTITPIIHSNSSIALFIFTIIYFIVVLYICLNPSKLIDRIGSLLTPLLLITILAMIIKGYLDFSGNSAGKGNEALYHSNFSSFAEGFTQGYLTMDAIAAIAFSMIVVNAVKLTGITKTNQIFKQTLTAGLIAAVALIFIYISLGYIGNHMPVSDMTLDQLKSKDRNIGTYLLTTMASTGFGSFGKYLLGIIVALACLTTACGLIVAVSEYFHRIVPKVSYKAFVLVFILMSFIIANQGLNAVISMSIPVLSIVYPVAITVVLLILIAKFIPTKRISQQIPVIIVFILSIFSVISKLGWLKINFIESLPLRAYSLEWFPVAIIATILGYLVGIFVKQDPIKYQQE
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS One: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e) - ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p)