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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1244 [new locus tag: SACOL_RS06360 ]
  • pan locus tag?: SAUPAN003514000
  • symbol: fabD
  • pan gene symbol?: fabD
  • synonym:
  • product: malonyl CoA-ACP transacylase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1244 [new locus tag: SACOL_RS06360 ]
  • symbol: fabD
  • product: malonyl CoA-ACP transacylase
  • replicon: chromosome
  • strand: +
  • coordinates: 1253415..1254341
  • length: 927
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAGTAAAACAGCAATTATTTTTCCGGGACAAGGTGCCCAAAAAGTTGGTATGGCGCAA
    GATTTGTTTAACAACAATGATCAAGCAACTGAAATTTTAACTTCAGCAGCGAACACATTA
    GACTTTGATATTTTAGAGACAATGTTTACTGATGAAGAAGGTAAATTGGGTGAAACTGAA
    AACACACAACCAGCTTTATTGACGCATAGTTCGGCATTATTAGCAGCGCTAAAAAATTTG
    AATCCTGATTTTACTATGGGGCATAGTTTAGGTGAATATTCAAGTTTAGTTGCAGCTGAC
    GTATTATCATTTGAAGATGCAGTTAAAATTGTTAGAAAACGTGGTCAATTAATGGCGCAA
    GCATTTCCTACTGGTGTAGGAAGCATGGCTGCAGTATTGGGATTAGATTTTGATAAAGTC
    GATGAAATTTGTAAGTCATTATCATCTGATGACAAAATAATTGAACCAGCAAACATTAAT
    TGCCCAGGTCAAATTGTTGTTTCAGGTCACAAAGCTTTAATTGATGAGCTAGTAGAAAAA
    GGTAAATCATTAGGTGCAAAACGTGTCATGCCTTTAGCAGTATCTGGACCATTCCATTCA
    TCGCTAATGAAAGTGATTGAAGAAGATTTTTCAAGTTACATTAATCAATTTGAATGGCGT
    GATGCTAAGTTTCCTGTAGTTCAAAATGTAAATGCGCAAGGTGAAACTGACAAAGAAGTA
    ATTAAATCTAATATGGTCAAGCAATTATATTCACCAGTACAATTCATTAACTCAACAGAA
    TGGCTAATAGACCAAGGTGTTGATCATTTTATTGAAATTGGTCCTGGAAAAGTTTTATCT
    GGCTTAATTAAAAAAATAAATAGAGATGTTAAGTTAACATCAATTCAAACTTTAGAAGAT
    GTGAAAGGATGGAATGAAAATGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    927

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1244 [new locus tag: SACOL_RS06360 ]
  • symbol: FabD
  • description: malonyl CoA-ACP transacylase
  • length: 308
  • theoretical pI: 4.52538
  • theoretical MW: 33635
  • GRAVY: -0.146753

⊟Function[edit | edit source]

  • reaction:
    EC 2.3.1.39?  ExPASy
    [Acyl-carrier-protein] S-malonyltransferase Malonyl-CoA + an [acyl-carrier-protein] = CoA + a malonyl-[acyl-carrier-protein]
  • TIGRFAM:
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis malonyl CoA-acyl carrier protein transacylase (TIGR00128; EC 2.3.1.39; HMM-score: 328.4)
    and 3 more
    malonate decarboxylase, epsilon subunit (TIGR03131; HMM-score: 201.2)
    polyketide-type polyunsaturated fatty acid synthase PfaA (TIGR02813; HMM-score: 116.5)
    PfaB family protein (TIGR02816; HMM-score: 16.5)
  • TheSEED  :
    • Malonyl CoA-acyl carrier protein transacylase (EC 2.3.1.39)
    Fatty Acids, Lipids, and Isoprenoids Fatty acids Fatty Acid Biosynthesis FASII  Malonyl CoA-acyl carrier protein transacylase (EC 2.3.1.39)
  • PFAM:
    Patatin (CL0323) Acyl_transf_1; Acyl transferase domain (PF00698; HMM-score: 123.7)
    and 1 more
    no clan defined V-ATPase_C; V-ATPase subunit C (PF03223; HMM-score: 11.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.010009
    • TAT(Tat/SPI): 0.000403
    • LIPO(Sec/SPII): 0.000698
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSKTAIIFPGQGAQKVGMAQDLFNNNDQATEILTSAANTLDFDILETMFTDEEGKLGETENTQPALLTHSSALLAALKNLNPDFTMGHSLGEYSSLVAADVLSFEDAVKIVRKRGQLMAQAFPTGVGSMAAVLGLDFDKVDEICKSLSSDDKIIEPANINCPGQIVVSGHKALIDELVEKGKSLGAKRVMPLAVSGPFHSSLMKVIEEDFSSYINQFEWRDAKFPVVQNVNAQGETDKEVIKSNMVKQLYSPVQFINSTEWLIDQGVDHFIEIGPGKVLSGLIKKINRDVKLTSIQTLEDVKGWNEND

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3] [4]
  • quantitative data / protein copy number per cell: 1008 [5]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: FapR* (repression) regulon
    FapR*(TF)important in Fatty acid biosynthesis; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: 43.83 h [6]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  5. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  6. ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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